andor_w1
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andor_w1 [2025/01/15 14:59] bioimaging |
andor_w1 [2025/07/16 16:36] (current) bioimaging |
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| <a href="/facility/bioimaging/lib/exe/fetch.php?media=andor_w1.jpg"><img src="/facility/bioimaging/lib/exe/fetch.php?media=andor_w1.jpg" width=300></a> | <a href="/facility/bioimaging/lib/exe/fetch.php?media=andor_w1.jpg"><img src="/facility/bioimaging/lib/exe/fetch.php?media=andor_w1.jpg" width=300></a> | ||
| <td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
| - | <b>Location</b>: [Oeiras] Room 0B05<br> | + | <b>Location</b>: no longer available<br> |
| <b>Manufacturer</b>: Andor<br> | <b>Manufacturer</b>: Andor<br> | ||
| <b>Model</b>: Revolution WD<br> | <b>Model</b>: Revolution WD<br> | ||
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| <b>SN</b>: <br> | <b>SN</b>: <br> | ||
|   <br> |   <br> | ||
| - | Data will be deleted after: <b>1 month</b> | ||
|   <br> |   <br> | ||
|   <br> |   <br> | ||
| - | → <a href="/facility/bioimaging/doku.php?id=andor_w1_quality"><img src="/facility/bioimaging/lib/exe/fetch.php?media=list-icon.png" width=18> Andor Spinning Disk W1 Quality Control</a> | ||
| - | <br> | ||
| - | → <a href="/facility/bioimaging/doku.php?id=andor_w1_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png"> Andor Spinning Disk W1 Usage Statistics</a> | ||
| </p> | </p> | ||
| </td> | </td> | ||
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| ==== Objectives ==== | ==== Objectives ==== | ||
| - | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ PFS compatible ^ DIC compatible ^ PhC compatible ^ Reference ^ | + | ^ Mag. ^ Model ^ Imm. ^ NA ^ WD (mm) ^ PFS? ^ DIC? ^ PhC? ^ Reference ^ |
| | 4x | Nikon Plan DL | - | 0.13 | 16.5 | N | N/A | Ph1 | [[https://www.microscope.healthcare.nikon.com/products/optics/selector/comparison/-1834|MRH20045]] | | | 4x | Nikon Plan DL | - | 0.13 | 16.5 | N | N/A | Ph1 | [[https://www.microscope.healthcare.nikon.com/products/optics/selector/comparison/-1834|MRH20045]] | | ||
| - | | 10x | Nikon Plan DL | - | 0.25 | 10.5 | Yes | Not recommended | Ph1 | [[https://www.microscope.healthcare.nikon.com/products/optics/selector/comparison/-1701|MRL20102]] | | + | | 10x | Nikon Plan DL | - | 0.25 | 10.5 | Yes | No | Ph1 | [[https://www.microscope.healthcare.nikon.com/products/optics/selector/comparison/-1701|MRL20102]] | |
| - | | 20x | Nikon Plan Fluor | - | 0.5 | 2.1 | Yes | Not recommended | Ph1 | [[https://www.microscope.healthcare.nikon.com/products/optics/selector/comparison/-1837|MRH10205]] | | + | | 20x | Nikon Plan Fluor | - | 0.5 | 2.1 | Yes | No | Ph1 | [[https://www.microscope.healthcare.nikon.com/products/optics/selector/comparison/-1837|MRH10205]] | |
| | 20x | Nikon Plan Fluor | MImm | 0.75 | 0.35 | No | Yes (N1) | No | [[https://www.microscope.healthcare.nikon.com/products/optics/selector/comparison/-1827|MRH07241]] | | | 20x | Nikon Plan Fluor | MImm | 0.75 | 0.35 | No | Yes (N1) | No | [[https://www.microscope.healthcare.nikon.com/products/optics/selector/comparison/-1827|MRH07241]] | | ||
| | 40x | Plan Fluor | Oil | 1.3 | 0.24 | Yes | Yes (N2) | No | [[https://www.microscope.healthcare.nikon.com/en_EU/products/optics/selector/comparison/-1829|MRH01401]] | | | 40x | Plan Fluor | Oil | 1.3 | 0.24 | Yes | Yes (N2) | No | [[https://www.microscope.healthcare.nikon.com/en_EU/products/optics/selector/comparison/-1829|MRH01401]] | | ||
| | 63x | Nikon Plan APO VC | W | 1.20 | 0.175 | No | No | No | [[https://www.microscope.healthcare.nikon.com/products/optics/selector/comparison/-1855|MRD07602]] | | | 63x | Nikon Plan APO VC | W | 1.20 | 0.175 | No | No | No | [[https://www.microscope.healthcare.nikon.com/products/optics/selector/comparison/-1855|MRD07602]] | | ||
| - | |||
| - | * Not fabricated anymore ; * * LWD - Long Working Distance | ||
| ==== CSU Dichroics ==== | ==== CSU Dichroics ==== | ||
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| | 1 | [[https://www.idex-hs.com/store/product-detail/ff685_di02_25x36/fl-007099|IR mirror]] | 685LP | | | 1 | [[https://www.idex-hs.com/store/product-detail/ff685_di02_25x36/fl-007099|IR mirror]] | 685LP | | ||
| | 2 | [[https://www.idex-hs.com/store/product-detail/di01_t405_488_568_647_13x15x0_5/fl-006984|LD 405/488/561/640]] | Quad mirror 80-85% transmission| | | 2 | [[https://www.idex-hs.com/store/product-detail/di01_t405_488_568_647_13x15x0_5/fl-006984|LD 405/488/561/640]] | Quad mirror 80-85% transmission| | ||
| - | | 3 | [[https://www.chroma.com/products/parts/zt405-488-561-640rpc|ZT 405/488/561/640]] | Quad mirror >95% transmission | | + | | 3 | [[https://www.chroma.com/products/parts/zt405-488-561-640rpc|ZT 405/488/561/640]] | Quad mirror >95% transmission "HE" | |
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| ==== turning on ==== | ==== turning on ==== | ||
| - | * 1st: Turn on the cameras water cooling system (1 switches) and wait until it cools down to 15ºC. This should turn on both components of the water cooling system, if this does not happen, tell us as soon as possible! | + | * 1st: Turn on the cameras water cooling system (1 switches) and wait until it cools down to 15ºC. This should turn on both components of the water cooling system, if this does not happen, tell us as soon as possible! **__Important__**: This should be done at least 10 to 15min before starting and you cannot start until it's at 15ºC. |
| - | **Important**: This should be done at least 10 to 15min before starting and you cannot start until it's at 15ºC. | + | * Turn on the computer. |
| - | * Turn on the computer. | + | * Log-In to the computer using your Agendo credentials (Username and Password). |
| - | * Log-In to the computer using your Agendo credentials (Username and Password). | + | * Turn on the Main Cabinet key and switches. |
| - | * Turn on the Main Cabinet key and switches. | + | * Turn on the Power Bars. |
| - | * Turn on the Power Bars. | + | * Turn on the Spinning Disk scanhead by rotating the key. |
| - | * Turn on the Spinning Disk scanhead by rotating the key. | + | * Turn on the cameras. Only if the cooler is at 15ºC. |
| - | * Turn on the cameras. Only if the cooler is at 15ºC. | + | * Press the button for the EMCCD and the switch for the sCMOS. |
| - | * Press the button for the EMCCD and the switch for the sCMOS. | + | * Start the iQ and input your iQ username and passowrd (it is different from the Agendo one!). If the software asks to initialize the stage, please do it BUT make sure the objective is "escaped" (click escape button on the right side of the microscope and when it's done click refocus to return to the previous focus). |
| - | * Start the iQ and input your iQ username and passowrd (it is different from the Agendo one!). If the software asks to initialize the stage, please do it BUT make sure the objective is "escaped" (click escape button on the right side of the microscope and when it's done click refocus to return to the previous focus). | + | |
| * Remember to choose the proper calibration if doing montages/size measurements. | * Remember to choose the proper calibration if doing montages/size measurements. | ||
| * In iQ you need a different calibration for each objective and to check if whether you are using the 1,5x auxiliary Optovar magnification or not (selector in bottom right of the microscope front panel). | * In iQ you need a different calibration for each objective and to check if whether you are using the 1,5x auxiliary Optovar magnification or not (selector in bottom right of the microscope front panel). | ||
andor_w1.1736949578.txt.gz · Last modified: 2025/01/15 14:59 by bioimaging
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