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about [2025/07/16 16:24] bioimaging |
about [2026/02/04 11:28] (current) bioimaging [Contacts] |
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| <img src="/facility/bioimaging/lib/exe/fetch.php?media=bioimaging_systems_2025.gif" width=340 align=left> | <img src="/facility/bioimaging/lib/exe/fetch.php?media=bioimaging_systems_2025.gif" width=340 align=left> | ||
| - | </html>The facility houses 29 microscopy systems, including five inverted laser point scanning confocal microscopes with motorized stage, piezo and super-resolution detector prepared for live cell imaging [3x [[https://www.zeiss.com/microscopy/en/products/light-microscopes/confocal-microscopes/lsm-980-with-airyscan-2.html|ZEISS LSM 980 with Airyscan 2]] (one of them with a 32-channel spectral detector), [[zeiss_lsm_900|ZEISS LSM 900 with Airyscan 2]], [[zeiss_lsm_880|ZEISS LSM 880 with Airyscan]]), and one fully automated inverted widefield and point scanning confocal microscope optimized for high-throughput live imaging in well-plates ([[zeiss_celldiscoverer_7|ZEISS Celldiscoverer 7 with LSM 900]])], six inverted spinning disk confocal microscopes with motorized stage, piezo and EM-CCD/sCMOS cameras prepared for live cell imaging [3x [[https://www.intelligent-imaging.com/marianas|3i Marianas SDC]] (one of them with TIRF and in a BSL2 room), [[https://andor.oxinst.com/products/dragonfly-confocal-microscope-system|Andor Dragonfly SDC]], [[leica_sdc|Leica Spinning Disk Confocal]], and [[zeiss_cell_observer_sd|ZEISS Cell Observer SD]]], three light sheet microscopes prepared for water and clearing media immersion imaging (2x ZEISS Lightsheet Z.1, and [[blaze|Miltenyi UltraMicroscope Blaze]]), one multi-photon microscope ([[prairie|Prairie Multiphoton]]), seven fully motorized inverted widefield microscopes with cooled CCD/CMOS cameras ([[https://www.agilent.com/en/product/cell-analysis/cell-imaging-microscopy/cell-imaging-multimode-readers/biotek-cytation-5-cell-imaging-multimode-reader-1623202|Agilent Cytation 5]] multimode reader with incubation, [[deltavision|Deltavision OMX-SIM]] super-resolution with incubation and SIM-TIRF, [[nikon_eclipse_ti|Nikon Eclipse Ti]] with stage top incubation and a Ionoptix calcium and contractility measurement system, [[nikon_htm-hcs|Nikon HTM-HCS]] with incubation, [[zeiss_cell_observer|ZEISS Cell Observer]] with a large cage incubator and an Akoya PhenoCycler for spatial phenotyping, [[https://www.zeiss.com/microscopy/en/products/light-microscopes/widefield-microscopes/axio-imager-2-for-life-science-research.html|ZEISS Imager with Apotome 2]] with 8 slide carrier, and ZEISS Axiovert 200M), one automated slide scanner for fluorescence and brightfield scanning up to 100 slides ([[https://www.zeiss.com/microscopy/en/products/imaging-systems/axioscan-for-biology.html|ZEISS Axioscan 7]]), two fluorescence stereo microscopes with CCD/CMOS cameras ([[https://www.zeiss.com/microscopy/en/products/light-microscopes/stereo-and-zoom-microscopes/axio-zoom-v16-for-biology.html|ZEISS Axio Zoom.V16]], and [[oeiras_zeiss_lumar_v12|ZEISS Stereo Lumar.V12]]) and other imaging resources such as a brightfield microscope ([[leica_dm2500|Leica DM2500]]) and a bioluminescence/fluorescence imaging system ([[ivis_lumina|IVIS Lumina]]). There are also high spec workstations for image analysis and processing ([[amira|Amira]], [[chronos|Chronos]], [[huygens|Huygens]], [[imaris|Imaris]], [[z2_workstation|Z2]], [[z3_workstation|Z3]], and ZEISS LSM 980-32 Workstations). | + | </html>The Platform houses 29 microscopy systems, including five inverted laser point scanning confocal microscopes with motorized stage, piezo and super-resolution detector prepared for live cell imaging [3x [[https://www.zeiss.com/microscopy/en/products/light-microscopes/confocal-microscopes/lsm-980-with-airyscan-2.html|ZEISS LSM 980 with Airyscan 2]] (one of them with a 32-channel spectral detector), [[zeiss_lsm_900|ZEISS LSM 900 with Airyscan 2]], [[zeiss_lsm_880|ZEISS LSM 880 with Airyscan]]), and one fully automated inverted widefield and point scanning confocal microscope optimized for high-throughput live imaging in well-plates that integrates with an advanced robotic loader, automated liquid handler, and incubator to enable high-throughput, fully automated workflows for live-cell imaging ([[zeiss_celldiscoverer_7|ZEISS Celldiscoverer 7 with LSM 900]])], six inverted spinning disk confocal microscopes with motorized stage, piezo and EM-CCD/sCMOS cameras prepared for live cell imaging [3x [[https://www.intelligent-imaging.com/marianas|3i Marianas SDC]] (one of them with TIRF and in a BSL2 room), [[https://andor.oxinst.com/products/dragonfly-confocal-microscope-system|Andor Dragonfly SDC]], [[leica_sdc|Leica Spinning Disk Confocal]], and [[zeiss_cell_observer_sd|ZEISS Cell Observer SD]]], three light sheet microscopes prepared for water and clearing media immersion imaging (2x ZEISS Lightsheet Z.1, and [[blaze|Miltenyi UltraMicroscope Blaze]]), one multi-photon microscope ([[prairie|Prairie Multiphoton]]), seven fully motorized inverted widefield microscopes with cooled CCD/CMOS cameras ([[https://www.agilent.com/en/product/cell-analysis/cell-imaging-microscopy/cell-imaging-multimode-readers/biotek-cytation-5-cell-imaging-multimode-reader-1623202|Agilent Cytation 5]] multimode reader with incubation, [[deltavision|Deltavision OMX-SIM]] super-resolution with incubation and SIM-TIRF, [[nikon_eclipse_ti|Nikon Eclipse Ti]] with stage top incubation, [[nikon_htm-hcs|Nikon HTM-HCS]] with incubation and a Ionoptix calcium and contractility measurement system, [[zeiss_cell_observer|ZEISS Cell Observer]] with a large cage incubator and an Akoya PhenoCycler for spatial phenotyping, [[https://www.zeiss.com/microscopy/en/products/light-microscopes/widefield-microscopes/axio-imager-2-for-life-science-research.html|ZEISS Imager with Apotome 2]] with 8 slide carrier, and ZEISS Axiovert 200M), one automated slide scanner for fluorescence and brightfield scanning up to 100 slides ([[https://www.zeiss.com/microscopy/en/products/imaging-systems/axioscan-for-biology.html|ZEISS Axioscan 7]]), two fluorescence stereo microscopes with CCD/CMOS cameras ([[https://www.zeiss.com/microscopy/en/products/light-microscopes/stereo-and-zoom-microscopes/axio-zoom-v16-for-biology.html|ZEISS Axio Zoom.V16]], and [[oeiras_zeiss_lumar_v12|ZEISS Stereo Lumar.V12]]) and other imaging resources such as a brightfield microscope ([[leica_dm2500|Leica DM2500]]) and a bioluminescence/fluorescence imaging system ([[ivis_lumina|IVIS Lumina]]). There are also high spec workstations for image analysis and processing ([[amira|Amira]], [[chronos|Chronos]], [[huygens|Huygens]], [[imaris|Imaris]], [[z2_workstation|Z2]], [[z3_workstation|Z3]], and ZEISS LSM 980-32 Workstations). |
| The Bioimaging systems are available on the [[https://gimm.agendoscience.com|online booking system]] for trained researchers 7 days a week and 24 hours per day. User training in each particular system is mandatory, even for researchers with previous microscopy experience. | The Bioimaging systems are available on the [[https://gimm.agendoscience.com|online booking system]] for trained researchers 7 days a week and 24 hours per day. User training in each particular system is mandatory, even for researchers with previous microscopy experience. | ||
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| <img src="/facility/bioimaging/lib/exe/fetch.php?media=lisbon_site.png" width=340 align=left><BR> | <img src="/facility/bioimaging/lib/exe/fetch.php?media=lisbon_site.png" width=340 align=left><BR> | ||
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|     <b>GIMM Lisbon Site</b><BR> |     <b>GIMM Lisbon Site</b><BR> | ||
| + |     <a href="/facility/bioimaging/lib/exe/fetch.php?media=bioimaging_map_2025.png">Room P3-A-5</a><BR> | ||
|     Edifício Egas Moniz<BR> |     Edifício Egas Moniz<BR> | ||
|     Avenida Professor Egas Moniz<BR> |     Avenida Professor Egas Moniz<BR> | ||
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| <img src="/facility/bioimaging/lib/exe/fetch.php?media=oeiras_site.png" width=250 align=left><BR> | <img src="/facility/bioimaging/lib/exe/fetch.php?media=oeiras_site.png" width=250 align=left><BR> | ||
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|     <b>GIMM Oeiras Site</b><BR> |     <b>GIMM Oeiras Site</b><BR> | ||
| + |     Bartolomeu Dias Wing<BR> | ||
|     Rua da Quinta Grande 6<BR> |     Rua da Quinta Grande 6<BR> | ||
|     2780-156 Oeiras, Portugal<BR> |     2780-156 Oeiras, Portugal<BR> | ||
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| === Hours === | === Hours === | ||
| - | **9:00 - 17:30** (with a 1h break for lunch) for **Training, Assistance and Consultations** | + | **9:00 - 17:30** (with a 1h break for lunch) for **Training, Assistance and Consultations** \\ |
| + | (except on **Wednesdays 9:00 - 12:00** - weekly all-hands team meeting) | ||
| 24 hours, 7 days a week for Equipment Usage by **authorized, trained users** (see [[usage_policy|Usage Policies]]) | 24 hours, 7 days a week for Equipment Usage by **authorized, trained users** (see [[usage_policy|Usage Policies]]) | ||