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--- about [2022/06/19 16:48]
bioimaging
+++ about [2026/02/04 11:28] (current)
bioimaging [Contacts]
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-====== About the Bioimaging Unit ======
+====== About the Bioimaging Platform ======
 <html>
-<iframe width="560" height="315" src="https://www.youtube.com/embed/9ntTKoIexF4" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture" allowfullscreen></iframe>
+<img src="/facility/bioimaging/lib/exe/fetch.php?media=bioimaging_support.png" width=470 align=left>
 </html>
-\\
-[[team|{{  ::ubi_team_2022.jpeg?0x300|}}]]The Bioimaging Unit acts as a support structure to help and nurture research done with Light Microscopy. The people we work with have amazing questions to answer. But if used incorrectly, a microscope can give you wrong answers. At the Bioimaging Unit we focus on people and their questions. We provide iMM scientists and visitors with excellence in scientific know-how and expertise in light microscopy methods, in a personalized approach. We sit with researchers to teach them, train them, create software tools for them and help them make the most out of the microscope. We provide support in all steps of research done with Light Microscopy. We help researchers get to that moment when something is seen for the first time. To see Science done right, see it happening. That’s unique.
-\\
-\\
-→ **Meet the** [[team|{{team_neg.png?0x35|}}]]
-We are a node of the [[https://www.ppbi.pt/joomla30/index.php/ppbi|Portuguese Platform of Bioimaging]], which integrates the European research infrastructure as a Biological Node of [[https://www.eurobioimaging.eu/|Euro-BioImaging ERIC]]. We are also a [[https://www.zeiss.com/microscopy/int/about-us/microscopy-customer-center/labs-location-partner.html|Zeiss labs@location Partner]] and one of the [[https://colife.eu/en/research/core-facilities/bioimaging/|COLife]] Bioimaging Core Facilities.
+The Bioimaging Platform offers state-of-the-art **personalized support** in all steps of research done with Light Microscopy at GIMM, housing 29 systems including point-scanning confocal, spinning disk confocal, multiphoton, light sheet, widefield, slide scanning, and advanced image analysis workstations.
+**From the very first question to the moment of discovery and publication, we sit with researchers to advise, teach, train, and assist in image acquisition, analysis and processing, empowering scientists with cutting-edge technology and expertise.**
+In addition to daily hands-on training of users, we organize regular courses and workshops in basic and advanced light microscopy techniques and bioimage analysis.
+We are a node of [[https://ppbi.pt/wordpress/|Portuguese Platform of Bioimaging (PPBI)]], a nationwide consortium of imaging facilities which integrates the European research infrastructure [[https://www.eurobioimaging.eu/|Euro-BioImaging ERIC]]. We are also a [[https://www.zeiss.com/microscopy/en/about-us/microscopy-customer-center.html|ZEISS labs@location Partner]], joining a community of advanced microscopy facilities providing in-depth knowledge, dedicated services, and scientific support to ZEISS customers.
+<html>
+<p style="text-align:center"><a href="http://www.ppbi.pt/joomla30/" target="_blank"><img src="/facility/bioimaging/lib/exe/fetch.php?media=ppbi.png" width=140></a> &nbsp <a href="https://www.eurobioimaging.eu/" target="_blank"><img src="/facility/bioimaging/lib/exe/fetch.php?media=eurobioimaging.png" width=200></a> &nbsp <a href="https://www.zeiss.com/microscopy/en/about-us/microscopy-customer-center.html#partners" target="_blank"><img src="/facility/bioimaging/lib/exe/fetch.php?media=labslocation.png" width=200></a> &nbsp </p>
+</html>
 ===== How to Acknowledge us =====
+<html>
+<img src="/facility/bioimaging/lib/exe/fetch.php?media=team_2025_right.png" width=320 align=right>
+</html>
 If you used our services, please acknowledge us in publications or other formal presentations. This is one of our key performance indicators and very important for future funding. You are welcome to use the following examples:
-**Materials and Methods**: "Microscopy experiments were performed at the Bioimaging Unit of Instituto de Medicina Molecular João Lobo Antunes."
+==== Materials and Methods ====
+“Microscopy experiments were performed at the Bioimaging Platform of the Gulbenkian Institute for Molecular Medicine.”
-**Acknowledgements**: "We would like to thank the Bioimaging Unit of Instituto de Medicina Molecular João Lobo Antunes for their technical support. We also acknowledge the funding PPBI-POCI-01-0145-FEDER-022122."
+==== Acknowledgements ====
+“We thank the technical support of the Bioimaging Platform of the Gulbenkian Institute for Molecular Medicine, funded by PPBI-POCI-01-0145-FEDER-022122.”
+==== Co-authorship ====
+Assistance provided above the technical or routine level, where the Platform staff provides scientific input and expertise in experimental set-up, acquisition, analysis or writing, should be recognized through co-authorship on resulting publications. Please check this [[https://imm.medicina.ulisboa.pt/facility/bioimaging/lib/exe/fetch.php?media=imaging_facility_guidelines_for_acknowledgements_all_logos_1_.pdf|Global Bioimaging poster]] for co-authorship guidelines and talk to us, we are happy to discuss possible co-authorship situations.
 Thank you!
+<html>
+<p style="text-align:center"><img src="/facility/bioimaging/lib/exe/fetch.php?media=funding.png" width=850 align=center></p>
+</html>
 ===== Services =====
-The Bioimaging Unit is equipped with 2 inverted laser point-scanning confocal microscopes prepared for live cell imaging ([[zeiss_lsm_880|Zeiss LSM 880]] and [[zeiss_lsm_710|Zeiss LSM 710]]), 1 lightsheet microscope prepared for water and clearing media immersion imaging ([[zeiss_lightsheet_z1|Zeiss Lightsheet Z1]]), 2 inverted spinning disk confocal microscopes with motorized stages, piezo and EM-CCDs also prepared for live cell imaging ([[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]]), 1 multi-photon microscope ([[leica_sp8_mp|Leica SP8 MP]]), 3 fully motorized inverted widefield microscopes with cooled CCD cameras ([[zeiss_cell_observer|Zeiss Cell Observer]] with a large cage incubator, [[nikon_eclipse_ti|Nikon Eclipse Ti]] with stage top incubation, and [[zeiss_axiovert_200m|Zeiss Axiovert 200M]]), 1 upright widefield fluorescence microscope with a CCD camera ([[leica_dm5000b|Leica DM5000B]]), 1 fluorescence stereo microscope with a CCD camera ([[zeiss_axiozoom_v16|Zeiss AxioZoom V16]]) and other imaging resources such as a brightfield microscope ([[leica_dm2500|Leica DM2500]]) and a bioluminescent/fluorescence imaging system ([[ivis_lumina|IVIS Lumina]]). There is also a high-spec workstation for image analysis and processing available for researchers to work on their imaging data ([[colossus|Colossus]]). The Bioimaging systems are available on the [[https://imm.agendo.science|online booking system]] for trained researchers 7 days a week and 24 hours per day. User training in each particular system is mandatory, even for researchers with previous microscopy experience.
+The Bioimaging Platform provides training and assistance in planning optical microscopy-oriented projects, choosing materials and equipment, preparing samples, acquiring data, analyzing and processing acquired images, presenting and publishing results.
-→ **More info on our** [[resources|{{equipment_neg.png?0x35|}}]]
+<html>
+<img src="/facility/bioimaging/lib/exe/fetch.php?media=bioimaging_systems_2025.gif" width=340 align=left>
+</html>The Platform houses 29 microscopy systems, including five inverted laser point scanning confocal microscopes with motorized stage, piezo and super-resolution detector prepared for live cell imaging [3x [[https://www.zeiss.com/microscopy/en/products/light-microscopes/confocal-microscopes/lsm-980-with-airyscan-2.html|ZEISS LSM 980 with Airyscan 2]] (one of them with a 32-channel spectral detector), [[zeiss_lsm_900|ZEISS LSM 900 with Airyscan 2]], [[zeiss_lsm_880|ZEISS LSM 880 with Airyscan]]), and one fully automated inverted widefield and point scanning confocal microscope optimized for high-throughput live imaging in well-plates that integrates with an advanced robotic loader, automated liquid handler, and incubator to enable high-throughput, fully automated workflows for live-cell imaging ([[zeiss_celldiscoverer_7|ZEISS Celldiscoverer 7 with LSM 900]])], six inverted spinning disk confocal microscopes with motorized stage, piezo and EM-CCD/sCMOS cameras prepared for live cell imaging [3x [[https://www.intelligent-imaging.com/marianas|3i Marianas SDC]] (one of them with TIRF and in a BSL2 room), [[https://andor.oxinst.com/products/dragonfly-confocal-microscope-system|Andor Dragonfly SDC]], [[leica_sdc|Leica Spinning Disk Confocal]], and [[zeiss_cell_observer_sd|ZEISS Cell Observer SD]]], three light sheet microscopes prepared for water and clearing media immersion imaging (2x ZEISS Lightsheet Z.1, and [[blaze|Miltenyi UltraMicroscope Blaze]]), one multi-photon microscope ([[prairie|Prairie Multiphoton]]), seven fully motorized inverted widefield microscopes with cooled CCD/CMOS cameras ([[https://www.agilent.com/en/product/cell-analysis/cell-imaging-microscopy/cell-imaging-multimode-readers/biotek-cytation-5-cell-imaging-multimode-reader-1623202|Agilent Cytation 5]] multimode reader with incubation, [[deltavision|Deltavision OMX-SIM]] super-resolution with incubation and SIM-TIRF, [[nikon_eclipse_ti|Nikon Eclipse Ti]] with stage top incubation, [[nikon_htm-hcs|Nikon HTM-HCS]] with incubation and a Ionoptix calcium and contractility measurement system, [[zeiss_cell_observer|ZEISS Cell Observer]] with a large cage incubator and an Akoya PhenoCycler for spatial phenotyping, [[https://www.zeiss.com/microscopy/en/products/light-microscopes/widefield-microscopes/axio-imager-2-for-life-science-research.html|ZEISS Imager with Apotome 2]] with 8 slide carrier, and ZEISS Axiovert 200M), one automated slide scanner for fluorescence and brightfield scanning up to 100 slides ([[https://www.zeiss.com/microscopy/en/products/imaging-systems/axioscan-for-biology.html|ZEISS Axioscan 7]]), two fluorescence stereo microscopes with CCD/CMOS cameras ([[https://www.zeiss.com/microscopy/en/products/light-microscopes/stereo-and-zoom-microscopes/axio-zoom-v16-for-biology.html|ZEISS Axio Zoom.V16]], and [[oeiras_zeiss_lumar_v12|ZEISS Stereo Lumar.V12]]) and other imaging resources such as a brightfield microscope ([[leica_dm2500|Leica DM2500]]) and a bioluminescence/fluorescence imaging system ([[ivis_lumina|IVIS Lumina]]). There are also high spec workstations for image analysis and processing ([[amira|Amira]], [[chronos|Chronos]], [[huygens|Huygens]], [[imaris|Imaris]], [[z2_workstation|Z2]], [[z3_workstation|Z3]], and ZEISS LSM 980-32 Workstations).
-The Bioimaging Unit provides support in all steps of research done with Light Microscopy at the iMM, from the first ideas in Project Planning to the final Data Publication, with a strong focus in Microscope Training and Support and Image Analysis and Processing.
+The Bioimaging systems are available on the [[https://gimm.agendoscience.com|online booking system]] for trained researchers 7 days a week and 24 hours per day. User training in each particular system is mandatory, even for researchers with previous microscopy experience.
-{{process_en_dark.png|}}
+→ **More info on our**  <html><a href="/facility/bioimaging/doku.php?id=resources"><img src="/facility/bioimaging/lib/exe/fetch.php?media=microscope_section.png" width=180></a></html>
 ===== Prices =====
-We charge according to booked time in each microscopy system. [[https://imm.agendo.science/|Online booking]] is mandatory for each usage session and cancellations are allowed if they are made prior to reserved time. Users that do not show up at their booked sessions will be charged in full.
+We charge according to booked time in each microscopy system. [[https://gimm.agendoscience.com/|Online booking]] is mandatory for each usage session and cancellations are allowed if they are made prior to reserved time. Users that do not show up at their booked sessions will be charged in full.
-→ **Check our** [[billing|{{charge_rates_neg.png?0x35|}}]]
+→ **Check our**  <html><a href="/facility/bioimaging/doku.php?id=billing"><img src="/facility/bioimaging/lib/exe/fetch.php?media=charges_section.png" width=150></a></html>
 ===== How to use the service =====
-All new users must contact the Bioimaging Unit for training in each specific system. This also applies to researchers with previous experience in microscopy. Only trained users are allowed to book usage sessions on a particular microscope.
+All new users must contact the Bioimaging Platform for training in each specific system. This also applies to researchers with previous experience in microscopy. Only trained users are allowed to book usage sessions on a particular microscope.
-→ **How to** [[newuser|{{new_user_neg.png?0x35|}}]]
+→ **How to**  <html><a href="/facility/bioimaging/doku.php?id=newuser"><img src="/facility/bioimaging/lib/exe/fetch.php?media=getting_started_section.png" width=180></a></html>
 ===== Contacts =====
-{{:bioimaging_team.jpg?400  |}}Bioimaging Unit \\
+GIMM Bioimaging Platform \\
-**imm-bioimaging@medicina.ulisboa.pt**
+**bioimaging@gimm.pt**
- Instituto de Medicina Molecular, room P3-A-5 \\
+<html>
- Faculdade de Medicina de Lisboa \\
+<img src="/facility/bioimaging/lib/exe/fetch.php?media=lisbon_site.png" width=340 align=left><BR>
- Av. Prof. Egas Moniz \\
+<BR>
--028 Lisboa \\
+<BR>
-\\
+<BR>
-\\
+<BR>
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+<BR>
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+&nbsp &nbsp <b>GIMM Lisbon Site</b><BR>
-\\
+&nbsp &nbsp <a href="/facility/bioimaging/lib/exe/fetch.php?media=bioimaging_map_2025.png">Room P3-A-5</a><BR>
-\\
+&nbsp &nbsp Edifício Egas Moniz<BR>
-Photo: Bioimaging Unit in 2016 by [[https://anadebarros.github.io/|Ana de Barros]]
+&nbsp &nbsp Avenida Professor Egas Moniz<BR>
-\\
+&nbsp &nbsp 1649-028 Lisboa, Portugal<BR>
-\\
+&nbsp &nbsp ☎ 47305
-\\
+</html>
-=== Hours ===
-**9:00 - 13:00 and 14:00 - 18:00** for **Training, Assistance and Consultations**
+<html>
+<img src="/facility/bioimaging/lib/exe/fetch.php?media=oeiras_site.png" width=250 align=left><BR>
+<BR>
+<BR>
+<BR>
+<BR>
+<BR>
+<BR>
+<BR>
+<BR>
+<BR>
+&nbsp &nbsp <b>GIMM Oeiras Site</b><BR>
+&nbsp &nbsp Bartolomeu Dias Wing<BR>
+&nbsp &nbsp Rua da Quinta Grande 6<BR>
+&nbsp &nbsp 2780-156 Oeiras, Portugal<BR>
+&nbsp &nbsp ☎ 214464533<BR>
+&nbsp &nbsp ☎ 4533
+</html>
-hours, 7 days a week for Equipment Usage by **authorized, trained users** (see [[usage_policy|Usage Policies]])
+=== Hours ===
+**9:00 - 17:30** (with a 1h break for lunch) for **Training, Assistance and Consultations** \\
+(except on **Wednesdays 9:00 - 12:00** - weekly all-hands team meeting)
-{{bioimaging_map_new.png|}}
+hours, 7 days a week for Equipment Usage by **authorized, trained users** (see [[usage_policy|Usage Policies]])
-{{phone_neg.png?20|}} Internal phone extension: 47305
 ----
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