This shows you the differences between two versions of the page.
Both sides previous revision Previous revision Next revision | Previous revision Next revision Both sides next revision | ||
zeiss_lsm_880 [2020/02/28 00:35] bioimaging [Microscope Turn On Procedure] |
zeiss_lsm_880 [2020/07/21 23:22] bioimaging |
||
---|---|---|---|
Line 16: | Line 16: | ||
  <br> |   <br> | ||
  <br> |   <br> | ||
- | → <a href="https://imm.medicina.ulisboa.pt/intranet/booking/128"><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png"> Zeiss LSM 880 Booking</a> | + | → <a href="https://my.agendo.science/calendar/?id=OGNhbGVuZGFycw=="><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png"> Zeiss LSM 880 Booking</a> |
<br> | <br> | ||
→ <a href="/facility/bioimaging/doku.php?id=zeiss_lsm_880_quality"><img src="/facility/bioimaging/lib/exe/fetch.php?media=list-icon.png" width=18> Zeiss LSM 880 Quality Control</a> | → <a href="/facility/bioimaging/doku.php?id=zeiss_lsm_880_quality"><img src="/facility/bioimaging/lib/exe/fetch.php?media=list-icon.png" width=18> Zeiss LSM 880 Quality Control</a> | ||
Line 37: | Line 37: | ||
===== Booking Rules ===== | ===== Booking Rules ===== | ||
- | The Zeiss LSM 880 booking is divided in three daily slots: | + | - **Users** can book at maximum **8 hours per week** |
- | | 9:00 to 13:00 | 13:00 to 17:00 | 17:00 to 21:00 | | + | - This usage restriction does not apply for **weekends** and for working days **before 9:00** and **after 21:00** |
- | + | ||
- | - A **user** can book at maximum **2 sessions per week** | + | |
- | - Each **session must fit inside one of the three daily slots** (i.e. a session booked from 11:00 to 14:00 counts as 2 sessions) | + | |
- | - The above rules do not apply for **weekends** and for work days **before 9:00** and **after 21:00** | + | |
- | - The above rules do not apply for sessions booked on the very same day or **24 hours ahead** | + | |
- Exceptions to these rules require **approval from [[joserino@medicina.ulisboa.pt|José Rino]]**. | - Exceptions to these rules require **approval from [[joserino@medicina.ulisboa.pt|José Rino]]**. | ||
- | - Users must indicate which lasers they are going to use in the booking Description field | ||
- | - Users must indicate if they will use the incubator in the booking Description field (write down 37C INCUBATOR) | ||
===== System components ===== | ===== System components ===== | ||
Line 74: | Line 67: | ||
==== Filtersets (Ocular) ==== | ==== Filtersets (Ocular) ==== | ||
- | ^ Position ^ Filterset ^ Reference ^ Excitation ^ Emission ^ | + | ^ Position ^ Filterset ^ Reference ^ Excitation ^ Dichroic ^ Emission ^ |
- | | 1 | Green | [[https://www.micro-shop.zeiss.com/?s=7139069fdc79c&l=en&p=us&f=f&a=v&b=f&id=489038-9901-000&o=|FS38HE]] | 450-490 nm | 500-550 nm | | + | | 1 | Green | [[https://www.micro-shop.zeiss.com/?s=7139069fdc79c&l=en&p=us&f=f&a=v&b=f&id=489038-9901-000&o=|FS38HE]] | 450-490 nm | 495 nm | 500-550 nm | |
- | | 2 | Red | [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=000000-1114-101&o=|FS43]] | 533-558 nm | 570-640 nm | | + | | 2 | Red | [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=000000-1114-101&o=|FS43]] | 533-558 nm | 570 nm | 570-640 nm | |
- | | 3 | Blue | [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=488049-9901-000&o=|FS49]] | G 365 nm | 420-470 nm | | + | | 3 | Blue | [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=488049-9901-000&o=|FS49]] | G 365 nm | 395 nm | 420-470 nm | |
===== Microscope Turn On Procedure ===== | ===== Microscope Turn On Procedure ===== |