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zeiss_lsm_880 [2020/01/01 18:11] bioimaging [Objectives] |
zeiss_lsm_880 [2020/01/17 12:36] bioimaging |
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- Users must indicate which lasers they are going to use in the booking Description field | - Users must indicate which lasers they are going to use in the booking Description field | ||
- Users must indicate if they will use the incubator in the booking Description field (write down 37C INCUBATOR) | - Users must indicate if they will use the incubator in the booking Description field (write down 37C INCUBATOR) | ||
+ | |||
===== System components ===== | ===== System components ===== | ||
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**Upon request:** | **Upon request:** | ||
- | ^ Magnification ^ Model ^ Type ^ NA ^ WD (mm) ^ | + | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ |
- | | 25x | [[https://www.micro-shop.zeiss.com/?s=129353259d52f12&l=en&p=uk&f=o&a=v&m=s&id=420852-9972-000|LCI Plan-Neofluar]] | Oil/Glyc/W | 0.80 | 0.21 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420852-9972-000/Objective-LCI-Plan-Neofluar-25x-0.8-Imm-Corr-DIC-M27|420852-9972-000]] | | + | | 25x | LCI Plan-Neofluar Corr DIC | Oil/Glyc/W | 0.80 | 0.21 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420852-9973-000/Objective-LCI-Plan-Neofluar-25x-0.8-Imm-Corr-DIC-M27|420852-9972-000]] | |
- | | 40x | [[https://www.micro-shop.zeiss.com/?s=129353259d52f12&l=en&p=uk&f=o&a=v&m=s&id=420660-9970-000|Plan-Apochromat]] | Dry | 0.95 | 0.25 | | + | | 40x | Plan-Apochromat Corr | Dry | 0.95 | 0.25 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420660-9970-000/Objective-Plan-Apochromat-40x-0.95-Corr-M27|420660-9970-000]] | |
- | | 100x | [[https://www.micro-shop.zeiss.com/?s=7138707e1a1de&l=en&p=uk&f=o&a=v&m=s&id=420792-9800-000|alpha Plan-Apochromat]] | Oil | 1.46 | 0.11 | | + | | 100x | α Plan-Apochromat DIC | Oil | 1.46 | 0.11 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420792-9800-000/Objective-alpha-Plan-Apochromat-100x-1.46-Oil-DIC-M27|420792-9800-000]] | |
==== Filtersets (Ocular) ==== | ==== Filtersets (Ocular) ==== | ||
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* Turn on the two switches: ''SYSTEMS/PC'' and ''COMPONENTS'' | * Turn on the two switches: ''SYSTEMS/PC'' and ''COMPONENTS'' | ||
{{zeiss_lsm_880_1.jpg?100}} | {{zeiss_lsm_880_1.jpg?100}} | ||
- | * Turn the computer on | + | * Turn on the computer |
- | * Login with lsm 880 user account | + | * Login in to Windows (Bioimaging User) |
- | * Wait for network icon to change to ''connected'' state | + | * Check that the computer is connected to an ''Unidentified network'' (click on the network icon in the taskbar) |
+ | {{::network_icon.png?200}} | ||
* Start the **ZEN** software | * Start the **ZEN** software | ||
===== Microscope Turn Off Procedure ===== | ===== Microscope Turn Off Procedure ===== | ||
- | If there's another user for this microscope in the next hour: | + | If there is another user for this microscope in the __next hour__: |
- | * Close the software, leave the lasers on and log off the computer. | + | * Exit the ZEN software, leave the lasers on |
- | * Clean up any objective where you used immersion oil. | + | * Log off the computer |
- | * **Make sure that there really is another user going to use the microscope.** | + | * Clean up immersion objectives |
Else: | Else: | ||
* Turn off the lasers on **ZEN** | * Turn off the lasers on **ZEN** | ||
- | * Clean up any objective were you used immersion oil. | + | * Clean up immersion objectives |
- | * Exit the software, shut down the computer. | + | * Exit the ZEN software |
- | * Turn off the main switches ''SYSTEMS/PC'' and ''COMPONENTS'' | + | * Shutdown the computer |
- | * Wait 5 min. for Ar laser cooldown | + | * Turn off the two switches ''SYSTEMS/PC'' and ''COMPONENTS'' |
- | * Turn off the ''MAIN SWITCH''. | + | * **Wait 5 min. for Ar laser to cool down** |
+ | * Turn off the ''MAIN SWITCH'' | ||
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[[resources|Back to the Resources page]] | [[resources|Back to the Resources page]] |