Differences

This shows you the differences between two versions of the page.

--- zeiss_lsm_880 [2016/06/20 17:05]
bioimaging [Objectives]
+++ zeiss_lsm_880 [2017/10/12 11:16]
bioimaging [LASERs]
@@ Line 9: / Line 9: @@
 <b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">Carl Zeiss MicroImaging</a><br>
 <b>Model</b>: <a href="http://www.zeiss.com/microscopy/en_de/products/confocal-microscopes/lsm-880-with-airyscan-.html" target="_blank">LSM 880 with Airyscan</a><br>
+<b>Nickname</b>: "880"<br>
 <b>Software</b>: ZEN 2.1 (black) <br>
 <b>Year</b>: 2016 <br>
 <b>SN</b>: 2811000267 <br>
-&nbsp <br>
 &nbsp <br>
 &nbsp <br>
@@ Line 29: / Line 29: @@
 ===== Microscope overview =====
-{{  :airyscan.jpg?0x380}} The Zeiss LSM 880 with Airyscan is a //confocal point-scanning microscope// able to generate high-resolution three-dimensional images of thick specimens with high sensitivity and low photodamage. It is an inverted microscope specially suitable for live cell imaging and photobleaching experiments, equipped with a large size incubator for temperature control and CO2 supply and with Definite Focus for hardware focus control during long time-lapse acquisitions. Its [[http://www.zeiss.com/content/dam/Microscopy/Products/laser-scan/LSM%20880/zeiss-lsm880-airyscan-principle-672.jpg|Airyscan]] 32 channel area detector is able to collect all light from an Airy pattern simultaneously, allowing for increased resolution (140 nm laterally and 400 nm axially, at 488 nm). Its scanning unit further includes two PMT detectors (one of them cooled) as well as a GaAsP detector for increased sensitivity (45% QE compared to ~25% QE for conventional PMT).  It is equipped with lasers from violet to far red (405, 458, 488, 514, 561, 594 and 633 nm excitation wavelengths). With this system you can perform optical sectioning of fluorescent samples which are too thick for a widefield system such as the [[zeiss_cell_observer_wf|Zeiss Cell Observer WF]] or the [[zeiss_axiovert_200m|Zeiss Axiovert 200M]]. Image resolution with Airyscan is higher than the [[zeiss_lsm_710|Zeiss LSM 710]] and detection sensitivity is also higher than faster confocal systems such as the spinning disk [[3i_marianas_sdc|3i Marianas SDC]] or the //line scanning// [[zeiss_lsm_7_live|Zeiss LSM 7 Live]]. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]].
+{{  :airyscan.jpg?0x380}} The Zeiss LSM 880 with Airyscan is a //confocal point-scanning microscope// able to generate high-resolution three-dimensional images of thick specimens with high sensitivity and low photodamage. It is an inverted microscope specially suitable for live cell imaging and photobleaching experiments, equipped with a large size incubator for temperature control and CO2 supply and with Definite Focus for hardware focus control during long time-lapse acquisitions. Its [[http://www.zeiss.com/content/dam/Microscopy/Products/laser-scan/LSM%20880/zeiss-lsm880-airyscan-principle-672.jpg|Airyscan]] 32 channel area detector is able to collect all light from an Airy pattern simultaneously, allowing for increased resolution (140 nm laterally and 400 nm axially, at 488 nm). Its scanning unit further includes two PMT detectors (one of them cooled) as well as a GaAsP detector for increased sensitivity (45% QE compared to ~25% QE for conventional PMT).  It is equipped with lasers from violet to far red (405, 458, 488, 514, 561, 594 and 633 nm excitation wavelengths). With this system you can perform optical sectioning of fluorescent samples which are too thick for a widefield system such as the [[zeiss_cell_observer|Zeiss Cell Observer]] or the [[zeiss_axiovert_200m|Zeiss Axiovert 200M]]. Image resolution with Airyscan is higher than the [[zeiss_lsm_710|Zeiss LSM 710]] and detection sensitivity is also higher than faster confocal systems such as the spinning disk [[3i_marianas_sdc|3i Marianas SDC]] or the //line scanning// [[zeiss_lsm_7_live|Zeiss LSM 7 Live]]. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]].
 {{info.gif?|}} Click on the image on the left to see the system beam path in higher detail
@@ Line 44: / Line 44: @@
   - Users must indicate which lasers they are going to use in the booking Description field
   - Users must indicate if they will use the incubator in the booking Description field (write down 37C INCUBATOR)
-  - Users must write their internal extension phone number in the booking Description field
 ===== System components =====
@@ Line 60: / Line 58: @@
 |  10x  |  [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=420340-9901-000|EC Plan-Neofluar]]  |  Dry  |  0.30  |  5.2  |
 |  20x  |  [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=420650-9901-000|Plan-Apochromat]]  |  Dry  |  0.80  |  0.55  |
-|  25x  |  [[https://www.micro-shop.zeiss.com/?s=129353259d52f12&l=en&p=uk&f=o&a=v&m=s&id=420852-9972-000|LCI Plan-Neofluar]]  |  Oil/Glyc/W  |  0.80  |  0.21  |
 |  40x  |  [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=421767-9970-000|C-Apochromat]]  |  Water  |  1.20  |  0.28  |
 |  63x  |  [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=440762-9904-000|Plan-Apochromat]]  |  Oil  |  1.40  |  0.19  |
 **Upon request:**
 ^  Magnification   ^  Model  ^  Type  ^  NA  ^  WD (mm)  ^
+|  25x  |  [[https://www.micro-shop.zeiss.com/?s=129353259d52f12&l=en&p=uk&f=o&a=v&m=s&id=420852-9972-000|LCI Plan-Neofluar]]  |  Oil/Glyc/W  |  0.80  |  0.21  |
 |  40x  |  [[https://www.micro-shop.zeiss.com/?s=129353259d52f12&l=en&p=uk&f=o&a=v&m=s&id=420660-9970-000|Plan-Apochromat]]  |  Dry  |  0.95  |  0.25  |
 |  100x  |  [[https://www.micro-shop.zeiss.com/?s=7138707e1a1de&l=en&p=uk&f=o&a=v&m=s&id=420792-9800-000|alpha Plan-Apochromat]]  |  Oil  |  1.46  |  0.11  |
 ==== Filtersets (Ocular) ====

Bioimaging Wiki

User Tools

Site Tools

Differences

Page Tools