zeiss_lsm_880
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zeiss_lsm_880 [2016/04/05 00:35] bioimaging [Objectives] |
zeiss_lsm_880 [2016/06/29 13:10] bioimaging [Microscope overview] |
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| - | → <a href="https://imm.medicina.ulisboa.pt/intranet/booking/month.php?user=ZEISSLSM8&rid=128"><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png"> Zeiss LSM 880 Booking</a> | + |   <br> |
| + | → <a href="https://imm.medicina.ulisboa.pt/intranet/booking/128"><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png"> Zeiss LSM 880 Booking</a> | ||
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| → <a href="/facility/bioimaging/doku.php?id=zeiss_lsm_880_quality"><img src="/facility/bioimaging/lib/exe/fetch.php?media=list-icon.png" width=18> Zeiss LSM 880 Quality Control</a> | → <a href="/facility/bioimaging/doku.php?id=zeiss_lsm_880_quality"><img src="/facility/bioimaging/lib/exe/fetch.php?media=list-icon.png" width=18> Zeiss LSM 880 Quality Control</a> | ||
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| → <a href="/facility/bioimaging/doku.php?id=zeiss_lsm_880_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png"> Zeiss LSM 880 Usage Statistics</a> | → <a href="/facility/bioimaging/doku.php?id=zeiss_lsm_880_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png"> Zeiss LSM 880 Usage Statistics</a> | ||
| - |   </br> | ||
| - | → <a href="/facility/bioimaging/doku.php?id=zeiss_lsm_880_repair"><img src="/facility/bioimaging/lib/exe/fetch.php?media=repair-icon.gif" width=16> Zeiss LSM 880 Repair History</a> | ||
| </p> | </p> | ||
| </td> | </td> | ||
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| ===== Microscope overview ===== | ===== Microscope overview ===== | ||
| - | The Zeiss LSM 880 with Airyscan is a //confocal point-scanning microscope// able to generate high-resolution three-dimensional images of thick specimens with high sensitivity and low photodamage. It is an inverted microscope specially suitable for live cell imaging and photobleaching experiments, equipped with a large size incubator for temperature control and CO2 supply. Its [[http://www.zeiss.com/content/dam/Microscopy/Products/laser-scan/LSM%20880/zeiss-lsm880-airyscan-principle-672.jpg|Airyscan]] 32 channel area detector is able to collect all light from an Airy pattern simultaneously, allowing for increased resolution (140 nm laterally and 400 nm axially, at 488 nm). Its scanning unit further includes two PMT detectors (one of them cooled) as well as a GaAsP detector for increased sensitivity (45% QE compared to ~25% QE for conventional PMT). It is equipped with lasers from violet to far red (405, 458, 488, 514, 561, 594 and 633 nm excitation wavelengths). With this system you can perform optical sectioning of fluorescent samples which are too thick for a widefield system such as the [[zeiss_cell_observer_wf|Zeiss Cell Observer WF]] or the [[zeiss_axiovert_200m|Zeiss Axiovert 200M]]. Image resolution with Airyscan is higher than the [[zeiss_lsm_710|Zeiss LSM 710]] and detection sensitivity is also higher than faster confocal systems such as the spinning disk [[3i_marianas_sdc|3i Marianas SDC]] or the //line scanning// [[zeiss_lsm_7_live|Zeiss LSM 7 Live]]. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]]. | + | {{ :airyscan.jpg?0x380}} The Zeiss LSM 880 with Airyscan is a //confocal point-scanning microscope// able to generate high-resolution three-dimensional images of thick specimens with high sensitivity and low photodamage. It is an inverted microscope specially suitable for live cell imaging and photobleaching experiments, equipped with a large size incubator for temperature control and CO2 supply and with Definite Focus for hardware focus control during long time-lapse acquisitions. Its [[http://www.zeiss.com/content/dam/Microscopy/Products/laser-scan/LSM%20880/zeiss-lsm880-airyscan-principle-672.jpg|Airyscan]] 32 channel area detector is able to collect all light from an Airy pattern simultaneously, allowing for increased resolution (140 nm laterally and 400 nm axially, at 488 nm). Its scanning unit further includes two PMT detectors (one of them cooled) as well as a GaAsP detector for increased sensitivity (45% QE compared to ~25% QE for conventional PMT). It is equipped with lasers from violet to far red (405, 458, 488, 514, 561, 594 and 633 nm excitation wavelengths). With this system you can perform optical sectioning of fluorescent samples which are too thick for a widefield system such as the [[zeiss_axio_observer|Zeiss Axio Observer]] or the [[zeiss_axiovert_200m|Zeiss Axiovert 200M]]. Image resolution with Airyscan is higher than the [[zeiss_lsm_710|Zeiss LSM 710]] and detection sensitivity is also higher than faster confocal systems such as the spinning disk [[3i_marianas_sdc|3i Marianas SDC]] or the //line scanning// [[zeiss_lsm_7_live|Zeiss LSM 7 Live]]. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]]. |
| + | |||
| + | {{info.gif?|}} Click on the image on the left to see the system beam path in higher detail | ||
| ===== Booking Rules ===== | ===== Booking Rules ===== | ||
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| | 10x | [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=420340-9901-000|EC Plan-Neofluar]] | Dry | 0.30 | 5.2 | | | 10x | [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=420340-9901-000|EC Plan-Neofluar]] | Dry | 0.30 | 5.2 | | ||
| | 20x | [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=420650-9901-000|Plan-Apochromat]] | Dry | 0.80 | 0.55 | | | 20x | [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=420650-9901-000|Plan-Apochromat]] | Dry | 0.80 | 0.55 | | ||
| + | | 25x | [[https://www.micro-shop.zeiss.com/?s=129353259d52f12&l=en&p=uk&f=o&a=v&m=s&id=420852-9972-000|LCI Plan-Neofluar]] | Oil/Glyc/W | 0.80 | 0.21 | | ||
| + | | 40x | [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=421767-9970-000|C-Apochromat]] | Water | 1.20 | 0.28 | | ||
| | 63x | [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=440762-9904-000|Plan-Apochromat]] | Oil | 1.40 | 0.19 | | | 63x | [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=440762-9904-000|Plan-Apochromat]] | Oil | 1.40 | 0.19 | | ||
| - | | 100x | [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=440782-9902-000|Plan-Apochromat]] | Oil | 1.40 | 0.17 | | ||
| **Upon request:** | **Upon request:** | ||
| ^ Magnification ^ Model ^ Type ^ NA ^ WD (mm) ^ | ^ Magnification ^ Model ^ Type ^ NA ^ WD (mm) ^ | ||
| - | | 25x | [[https://www.micro-shop.zeiss.com/?s=129353259d52f12&l=en&p=uk&f=o&a=v&m=s&id=420852-9972-000|LCI Plan-NeoFluar]] | Oil/Glyc/W | 0.8 | 0.21 | | + | | 40x | [[https://www.micro-shop.zeiss.com/?s=129353259d52f12&l=en&p=uk&f=o&a=v&m=s&id=420660-9970-000|Plan-Apochromat]] | Dry | 0.95 | 0.25 | |
| | 100x | [[https://www.micro-shop.zeiss.com/?s=7138707e1a1de&l=en&p=uk&f=o&a=v&m=s&id=420792-9800-000|alpha Plan-Apochromat]] | Oil | 1.46 | 0.11 | | | 100x | [[https://www.micro-shop.zeiss.com/?s=7138707e1a1de&l=en&p=uk&f=o&a=v&m=s&id=420792-9800-000|alpha Plan-Apochromat]] | Oil | 1.46 | 0.11 | | ||
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| ^ Position ^ Filterset ^ Reference ^ Excitation ^ Emission ^ | ^ Position ^ Filterset ^ Reference ^ Excitation ^ Emission ^ | ||
| - | | 1 | Green | [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=000000-1031-346&o=|FS38]] | 450-490 nm | 500-550 nm | | + | | 1 | Green | [[https://www.micro-shop.zeiss.com/?s=7139069fdc79c&l=en&p=us&f=f&a=v&b=f&id=489038-9901-000&o=|FS38HE]] | 450-490 nm | 500-550 nm | |
| | 2 | Red | [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=000000-1114-101&o=|FS43]] | 533-558 nm | 570-640 nm | | | 2 | Red | [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=000000-1114-101&o=|FS43]] | 533-558 nm | 570-640 nm | | ||
| | 3 | Blue | [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=488049-9901-000&o=|FS49]] | G 365 nm | 420-470 nm | | | 3 | Blue | [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=488049-9901-000&o=|FS49]] | G 365 nm | 420-470 nm | | ||
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| * Turn on the two switches: ''SYSTEMS/PC'' and ''COMPONENTS'' | * Turn on the two switches: ''SYSTEMS/PC'' and ''COMPONENTS'' | ||
| {{zeiss_lsm_880_1.jpg?100}} | {{zeiss_lsm_880_1.jpg?100}} | ||
| - | * Turn the metal halide power source on (if needed) | ||
| - | {{:bioimaging:halide_710.jpg?80}} | ||
| - | * Turn the Ar laser on using the key (if needed) | ||
| - | {{:bioimaging:laser_key_710.jpg?100}} | ||
| * Turn the computer on | * Turn the computer on | ||
| * Login with your unit account | * Login with your unit account | ||
| * Wait for network icon to change to ''connected'' state | * Wait for network icon to change to ''connected'' state | ||
| - | * Switch the Ar laser from ''idle'' ton ''run'' (don't change the ''current'') | + | * Start the **ZEN** software |
| - | {{:bioimaging:laser_run_710.jpg?100}} | + | |
| - | * Start the ''ZEN 2009'' software | + | |
| ===== Microscope Turn Off Procedure ===== | ===== Microscope Turn Off Procedure ===== | ||
| If there's another user for this microscope in the next hour: | If there's another user for this microscope in the next hour: | ||
| - | * Close the software, leave the lasers on an log off the computer. | + | * Close the software, leave the lasers on and log off the computer. |
| - | * Clean up any objective were you used immersion oil. | + | * Clean up any objective where you used immersion oil. |
| * **Make sure that there really is another user going to use the microscope.** | * **Make sure that there really is another user going to use the microscope.** | ||
| Else: | Else: | ||
| - | * Turn off the 561 nm laser and incubator in the software (if used) | + | * Turn off the lasers on **ZEN** |
| - | * Switch the Ar laser from ''run'' to ''idle'' (if used) | + | |
| - | * Turn off the Ar laser using the key (if used) | + | |
| * Clean up any objective were you used immersion oil. | * Clean up any objective were you used immersion oil. | ||
| * Exit the software, shut down the computer. | * Exit the software, shut down the computer. | ||
| - | * Turn off the metal halide lamp | ||
| * Turn off the main switches ''SYSTEMS/PC'' and ''COMPONENTS'' | * Turn off the main switches ''SYSTEMS/PC'' and ''COMPONENTS'' | ||
| * Wait 5 min. for Ar laser cooldown | * Wait 5 min. for Ar laser cooldown | ||
| * Turn off the ''MAIN SWITCH''. | * Turn off the ''MAIN SWITCH''. | ||
| + | |||
| ---- | ---- | ||
| [[resources|Back to the Resources page]] | [[resources|Back to the Resources page]] | ||
zeiss_lsm_880.txt · Last modified: 2024/01/28 11:47 by bioimaging
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