zeiss_lsm_880
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zeiss_lsm_880 [2016/04/05 00:32] bioimaging [LASERs] |
zeiss_lsm_880 [2016/04/05 00:42] bioimaging [Microscope Turn On Procedure] |
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| | 10x | [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=420340-9901-000|EC Plan-Neofluar]] | Dry | 0.30 | 5.2 | | | 10x | [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=420340-9901-000|EC Plan-Neofluar]] | Dry | 0.30 | 5.2 | | ||
| | 20x | [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=420650-9901-000|Plan-Apochromat]] | Dry | 0.80 | 0.55 | | | 20x | [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=420650-9901-000|Plan-Apochromat]] | Dry | 0.80 | 0.55 | | ||
| - | | 40x | [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=440452-9903-000|EC Plan-Neofluar]] | Oil | 1.30 | 0.20 | | ||
| | 63x | [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=440762-9904-000|Plan-Apochromat]] | Oil | 1.40 | 0.19 | | | 63x | [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=440762-9904-000|Plan-Apochromat]] | Oil | 1.40 | 0.19 | | ||
| + | | 100x | [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=440782-9902-000|Plan-Apochromat]] | Oil | 1.40 | 0.17 | | ||
| **Upon request:** | **Upon request:** | ||
| Line 66: | Line 66: | ||
| ^ Magnification ^ Model ^ Type ^ NA ^ WD (mm) ^ | ^ Magnification ^ Model ^ Type ^ NA ^ WD (mm) ^ | ||
| | 25x | [[https://www.micro-shop.zeiss.com/?s=129353259d52f12&l=en&p=uk&f=o&a=v&m=s&id=420852-9972-000|LCI Plan-NeoFluar]] | Oil/Glyc/W | 0.8 | 0.21 | | | 25x | [[https://www.micro-shop.zeiss.com/?s=129353259d52f12&l=en&p=uk&f=o&a=v&m=s&id=420852-9972-000|LCI Plan-NeoFluar]] | Oil/Glyc/W | 0.8 | 0.21 | | ||
| - | | 100x | [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=440782-9902-000|Plan-Apochromat]] | Oil | 1.40 | 0.17 | | + | | 100x | [[https://www.micro-shop.zeiss.com/?s=7138707e1a1de&l=en&p=uk&f=o&a=v&m=s&id=420792-9800-000|alpha Plan-Apochromat]] | Oil | 1.46 | 0.11 | |
| ==== Filtersets (Ocular) ==== | ==== Filtersets (Ocular) ==== | ||
| ^ Position ^ Filterset ^ Reference ^ Excitation ^ Emission ^ | ^ Position ^ Filterset ^ Reference ^ Excitation ^ Emission ^ | ||
| - | | 1 | Green | [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=000000-1031-346&o=|FS38]] | 450-490 nm | 500-550 nm | | + | | 1 | Green | [[https://www.micro-shop.zeiss.com/?s=7139069fdc79c&l=en&p=us&f=f&a=v&b=f&id=489038-9901-000&o=|FS38HE]] | 450-490 nm | 500-550 nm | |
| | 2 | Red | [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=000000-1114-101&o=|FS43]] | 533-558 nm | 570-640 nm | | | 2 | Red | [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=000000-1114-101&o=|FS43]] | 533-558 nm | 570-640 nm | | ||
| | 3 | Blue | [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=488049-9901-000&o=|FS49]] | G 365 nm | 420-470 nm | | | 3 | Blue | [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=488049-9901-000&o=|FS49]] | G 365 nm | 420-470 nm | | ||
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| * Turn on the two switches: ''SYSTEMS/PC'' and ''COMPONENTS'' | * Turn on the two switches: ''SYSTEMS/PC'' and ''COMPONENTS'' | ||
| {{zeiss_lsm_880_1.jpg?100}} | {{zeiss_lsm_880_1.jpg?100}} | ||
| - | * Turn the metal halide power source on (if needed) | ||
| - | {{:bioimaging:halide_710.jpg?80}} | ||
| - | * Turn the Ar laser on using the key (if needed) | ||
| - | {{:bioimaging:laser_key_710.jpg?100}} | ||
| * Turn the computer on | * Turn the computer on | ||
| * Login with your unit account | * Login with your unit account | ||
| * Wait for network icon to change to ''connected'' state | * Wait for network icon to change to ''connected'' state | ||
| - | * Switch the Ar laser from ''idle'' ton ''run'' (don't change the ''current'') | + | * Start the **ZEN** software |
| - | {{:bioimaging:laser_run_710.jpg?100}} | + | |
| - | * Start the ''ZEN 2009'' software | + | |
| ===== Microscope Turn Off Procedure ===== | ===== Microscope Turn Off Procedure ===== | ||
| If there's another user for this microscope in the next hour: | If there's another user for this microscope in the next hour: | ||
| - | * Close the software, leave the lasers on an log off the computer. | + | * Close the software, leave the lasers on and log off the computer. |
| - | * Clean up any objective were you used immersion oil. | + | * Clean up any objective where you used immersion oil. |
| * **Make sure that there really is another user going to use the microscope.** | * **Make sure that there really is another user going to use the microscope.** | ||
| Else: | Else: | ||
| - | * Turn off the 561 nm laser and incubator in the software (if used) | + | * Turn off the lasers on ''ZEN'' |
| - | * Switch the Ar laser from ''run'' to ''idle'' (if used) | + | |
| - | * Turn off the Ar laser using the key (if used) | + | |
| * Clean up any objective were you used immersion oil. | * Clean up any objective were you used immersion oil. | ||
| * Exit the software, shut down the computer. | * Exit the software, shut down the computer. | ||
| - | * Turn off the metal halide lamp | ||
| * Turn off the main switches ''SYSTEMS/PC'' and ''COMPONENTS'' | * Turn off the main switches ''SYSTEMS/PC'' and ''COMPONENTS'' | ||
| * Wait 5 min. for Ar laser cooldown | * Wait 5 min. for Ar laser cooldown | ||
| * Turn off the ''MAIN SWITCH''. | * Turn off the ''MAIN SWITCH''. | ||
| + | |||
| ---- | ---- | ||
| [[resources|Back to the Resources page]] | [[resources|Back to the Resources page]] | ||
zeiss_lsm_880.txt · Last modified: 2024/01/28 11:47 by bioimaging
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