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--- zeiss_lsm_880 [2016/04/05 00:18]
bioimaging
+++ zeiss_lsm_880 [2020/10/27 17:32]
bioimaging [Microscope Turn On Procedure]
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-<img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_lsm_880_name.jpg">
+<img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_lsm_880_name_new.png">
 <table style="border:0px solid white;">
 <tr style="border:0px solid white;">
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-<a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_lsm_880_wiki.jpg"><img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_lsm_880_wiki.jpg" width=300></a>
+<a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_lsm_880_wiki_new.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_lsm_880_wiki_new.png" width=300></a>
 <td style="border:0px solid white;"> <p style="line-height:1.8">
-<b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_lsm_880_map.jpg">Room P2-A-22</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47217) <br>
+<b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_lsm_880_map_new.png">Room P2-A-22</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 47217) <br>
 <b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">Carl Zeiss MicroImaging</a><br>
-<b>Model</b>: <a href="http://www.zeiss.com/microscopy/en_de/products/confocal-microscopes/lsm-880-with-airyscan-.html" target="_blank">LSM 880 with Airyscan</a><br>
+<b>Model</b>: LSM 880 with Airyscan<br>
-<b>Software</b>: ZEN 2.1 (black) <br>
+<b>Nickname</b>: "880"<br>
+<b>Software</b>: ZEN 2.3 SP1 (black edition) <br>
 <b>Year</b>: 2016 <br>
 <b>SN</b>: 2811000267 <br>
@@ Line 15: / Line 16: @@
 &nbsp <br>
 &nbsp <br>
-&rarr; <a href="https://imm.medicina.ulisboa.pt/intranet/booking/month.php?user=ZEISSLSM8&rid=128"><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png">  Zeiss LSM 880 Booking</a>
+&rarr; <a href="https://my.agendo.science/calendar/?id=OGNhbGVuZGFycw=="><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png">  Zeiss LSM 880 Booking</a>
  <br>
 &rarr; <a href="/facility/bioimaging/doku.php?id=zeiss_lsm_880_quality"><img src="/facility/bioimaging/lib/exe/fetch.php?media=list-icon.png" width=18> Zeiss LSM 880 Quality Control</a>
  <br>
 &rarr; <a href="/facility/bioimaging/doku.php?id=zeiss_lsm_880_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png">  Zeiss LSM 880 Usage Statistics</a>
-&nbsp </br>
-&rarr; <a href="/facility/bioimaging/doku.php?id=zeiss_lsm_880_repair"><img src="/facility/bioimaging/lib/exe/fetch.php?media=repair-icon.gif" width=16> Zeiss LSM 880 Repair History</a>
  </p>
 </td>
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 ===== Microscope overview =====
-The Zeiss LSM 880 with Airyscan is a //confocal point-scanning microscope// able to generate high-resolution three-dimensional images of thick specimens with high sensitivity and low photodamage. It is an inverted microscope specially suitable for live cell imaging and photobleaching experiments, equipped with a large size incubator for temperature control and CO2 supply. Its [[http://www.zeiss.com/content/dam/Microscopy/Products/laser-scan/LSM%20880/zeiss-lsm880-airyscan-principle-672.jpg|Airyscan]] 32 channel area detector is able to collect all light from an Airy pattern simultaneously, allowing for increased resolution (140 nm laterally and 400 nm axially, at 488 nm). Its scanning unit further includes two PMT detectors (one of them cooled for single photon counting) as well as a GaAsP detector.  It is equipped with lasers from violet to far red (405, 458, 488, 561 and 633 nm excitation wavelengths). With this system you can perform optical sectioning of fluorescent samples which are too thick for a widefield system such as the [[bioimaging:zeiss_axiovert_200m|Zeiss Axiovert 200M]]. Image resolution and detection sensitivity are higher than faster confocal systems such as a spinning disk or the [[bioimaging:zeiss_7_live|Zeiss LSM 7 Live]], but image acquisition is slower. If you need to use a 594 nm laser or a 40x water objective for higher working distances, check the [[bioimaging:zeiss_lsm510_meta|Zeiss LSM 510 META]] confocal point-scanning system. If your personal computer cannot handle all the data you collected, check out the [[bioimaging:bigguy|Big Guy]].
+{{  :airyscan_beampath.png?400}} The Zeiss LSM 880 with Airyscan is a [[https://imm.medicina.ulisboa.pt/facility/bioimaging/lib/exe/fetch.php?media=confocal.png|confocal point-scanning microscope]] able to generate high-resolution three-dimensional images of thick specimens with high sensitivity, high speed and low photodamage. It is an inverted microscope especially suitable for live cell imaging and photobleaching experiments, equipped with a large size incubator for temperature control and CO2 supply and with Definite Focus for hardware focus control during long time-lapse acquisitions. The stage is motorized and furthermore equipped with a piezo for Z displacement so fast 4D imaging is possible in multiple stage positions. Its [[http://www.zeiss.com/content/dam/Microscopy/Products/laser-scan/LSM%20880/zeiss-lsm880-airyscan-principle-672.jpg|Airyscan]] 32 channel area detector is able to collect all light from an Airy pattern simultaneously, allowing for increased resolution (140 nm laterally and 400 nm axially, at 488 nm). It can also be used in [[https://www.youtube.com/watch?v=ln6VUmLzLLc|Fast Mode]], which enables parallel excitation and detection of four image pixels. The result is a speed improvement by a factor of four, while maintaining the sensitivity of Airyscan and 1.5x resolution improvement. Its scanning unit further includes two PMT detectors (one of them cooled) as well as a GaAsP detector for increased sensitivity (45% QE compared to ~25% QE for conventional PMT). It is equipped with lasers from violet to far red (405, 458, 488, 514, 561, 594 and 633 nm excitation wavelengths).
+{{::fast_airyscan.png?420  }}With this system you can perform optical sectioning of fluorescent samples which are too thick for a widefield system such as the [[zeiss_cell_observer|Zeiss Cell Observer]] or the [[zeiss_axiovert_200m|Zeiss Axiovert 200M]]. Image resolution with Airyscan is higher than the [[zeiss_lsm_710|Zeiss LSM 710]] and detection sensitivity is also higher than the spinning disks [[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]]. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]].
+{{:info.png?25|}} Click [[https://imm.medicina.ulisboa.pt/facility/bioimaging/lib/exe/fetch.php?cache=&media=airyscan.jpg|here]] to see the system beam path in higher detail
+{{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link.
+===== Booking Rules =====
+  - **Users** can book at maximum **8 hours per week**
+  - This usage restriction does not apply for **weekends** and for working days **before 9:00** and **after 21:00**
+  - Exceptions to these rules require **approval from [[joserino@medicina.ulisboa.pt|José Rino]]**.
 ===== System components =====
 ==== LASERs ====
 ^  Laser Unit  ^  Wavelength  ^  Maximum Power  ^  Current Status  ^
-|  Sapphire 488-100  |  488 nm  |  100 mW  |  ok  |
+|  Diode 405-30  |  405 nm  |  30 mW  |  ok  |
-|  DPSS 561-40  |  561 nm  |  40 mW  |  ok  |
+|  Argon  |  458, 488 and 514 nm  |  25 mW  |  ok  |
+|  DPSS 561-20  |  561 nm  |  20 mW  |  ok  |
+|  HeNe594  |  594 nm  |  2 mW  |  ok  |
+|  HeNe633  |  633 nm  |  5 mW  |  ok  |
 ==== Objectives ====
-^  Magnification  ^  Model  ^  Type  ^  NA  ^  WD (mm)  ^
+^  Magnification   ^  Model  ^  Immersion  ^  NA  ^  WD (mm)  ^  Reference  ^
-|  10x  |  [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=440039-0000-000|W Achroplan Ph1]]  |  Water dipping  |  0.30  |  3.1  |
+|  10x  |  EC Plan-Neofluar  |  Air  |  0.30  |  5.20  |  [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420340-9901-000/Objective-EC-Plan-Neofluar-10x-0.30-M27|420340-9901-000]]  |
-|  10x  |  [[https://www.micro-shop.zeiss.com/?s=2065696c9e13e&l=en&p=uk&f=o&a=v&m=s&id=440330-0000-000|Plan-Neofluar]]  |  Dry  |  0.30  |  5.5  |
+|  20x  |  Plan-Apochromat  |  Air  |  0.80  |  0.55  |  [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420650-9901-000/Objective-Plan-Apochromat-20x-0.8-M27|420650-9901-000]]  |
-|  20x  |  [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=421452-9900-000|W Plan-Apochromat]]  |  Water  |  1.00  |  1.8  |
+|  40x  |  C-Apochromat Corr  |  Water  |  1.20  |  0.28  |  [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/421767-9970-000/Objective-C-Apochromat-40x-1.20-W-Corr-M27|421767-9970-000]]  |
-|  40x  |  [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=440090-9901-000|W Achroplan]]  |  Water dipping  |  0.80  |  3.6  |
+|  63x  |  Plan-Apochromat DIC  |  Oil  |  1.40  |  0.19  |  [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420782-9900-799/Objective-Plan-Apochromat-63x-1.4-Oil-DIC-M27|420782-9900-799]]  |
-|  40x  |  [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=440451-9903-000|Plan-Neofluar Ph3]]  |  Oil  |  1.30  |  0.20  |
-==== Filtersets (Ocular) ====
-^  Position  ^  Filterset  ^  Reference  ^  Excitation  ^  Emission  ^
+**Upon request:**
-|  2  |  Green  |  [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=488010-9901-000&o=|FS10]]  |  450-490 nm  |  515-565 nm  |
-|  3  |  Red  |  [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=488015-0000-000&o=|FS15]]  |  540-552 nm  |  > 590 nm  |
-|  4  |  Blue (for ablation*)  |  [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=488049-9901-000&o=|FS49]]  |  none  |  420-470 nm  |
-* Excitation filter has been removed
-==== Filter and Dichroic sets ====
+^  Magnification   ^  Model  ^  Immersion  ^  NA  ^  WD (mm)  ^  Reference  ^
-|  {{zeiss5livefilters0.jpg?100}}  |  {{zeiss5livefilters1.jpg?100}}  |
+|  25x  |  LCI Plan-Neofluar Corr DIC  |  Oil/Glyc/W  |  0.80  |  0.21  |  [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420852-9973-000/Objective-LCI-Plan-Neofluar-25x-0.8-Imm-Corr-DIC-M27|420852-9972-000]]  |
-|  Main configuration  |  Emission filters  |
+|  40x  |  Plan-Apochromat Corr  |  Dry  |  0.95  |  0.25  |  [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420660-9970-000/Objective-Plan-Apochromat-40x-0.95-Corr-M27|420660-9970-000]]  |
+|  100x  |  α Plan-Apochromat DIC  |  Oil  |  1.46  |  0.11  |  [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420792-9800-000/Objective-alpha-Plan-Apochromat-100x-1.46-Oil-DIC-M27|420792-9800-000]]  |
+==== Filtersets (Ocular) ====
-===== Microscope Turn On Procedures =====
+^  Position  ^  Filterset  ^  Reference  ^  Excitation  ^  Dichroic  ^  Emission  ^
+|  1  |  Green  |  [[https://www.micro-shop.zeiss.com/?s=7139069fdc79c&l=en&p=us&f=f&a=v&b=f&id=489038-9901-000&o=|FS38HE]]  |  450-490 nm  |  495 nm  |  500-550 nm  |
+|  2  |  Red  |  [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=000000-1114-101&o=|FS43]]  |  533-558 nm  |  570 nm  |  570-640 nm  |
+|  3  |  Blue  |  [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=488049-9901-000&o=|FS49]]  |  G 365 nm  |  395 nm  |  420-470 nm  |
-==== Normal Operation ====
+===== Microscope Turn On Procedure =====
+  * Turn on the ''MAIN SWITCH''
+  * Turn on the ''SYSTEMS/PC'' switch
+{{zeiss_lsm_880_1.jpg?100}}
+  * Turn on the computer
+  * Login in to Windows (Bioimaging User)
+  * Turn on the ''COMPONENTS'' switch
+  * Start the **ZEN Black** software
-  * The system must be turned on **30 min** before usage.
+===== Microscope Turn Off Procedure =====
-    * To start it turn on the two main switches: ''SYSTEM/PC'' and ''COMPONENTS''
+If there is another user for this microscope in the __next hour__:
-{{7live_switches.jpg?100|}}
+  * Exit the ZEN software, leave the lasers on
-  * Login to the computer, wait for the network icon to change to ''connected'' state
+  * Log off the computer
-  * Start the **ZEN 2009** software.
+  * Clean up immersion objectives
-**After the 30 min:**
-  * Turn on the metal halide lamp (white box to the left of the microscope).
-{{7live_excite.jpg?100|}}
-  * You may now start using the system.
-==== Live Imaging / Time Lapse Operation ====
-  * The system must be turned on **2 hours** before usage (in order to properly warm up scanning mirrors).
-    * To start it turn on the two main switches: **SYSTEM/PC** and **COMPONENTS**
-  * Login to the computer, wait for the network icon to change to ''connected'' state
-  * Start the **ZEN 2009** software.
-**After the 2 hours:**
-  * Turn on the metal halide lamp (white box to the left of the microscope).
-  * You may now start using the system.
-===== Microscope Turn Off procedures =====
-If there's another user for this microscope in the next two hours:
-  * Exit the **ZEN 2009** software, leave the lasers on and log off the computer.
-  * Clean up immersion objectives.
-  * **Make sure that there really is another user going to use the microscope.**
 Else:
-  * Turn off the lasers, wait 5 min for them to cooldown.
+  * Turn off the lasers on **ZEN**
-  * Exit the **ZEN 2009** software and shut down the computer.
+  * Clean up immersion objectives
-  * Clean up immersion objectives.
+  * Exit the ZEN software
-  * Turn off the metal halide lamp.
+  * Shutdown the computer
-  * Turn off the microscope.
+  * Turn off the two switches ''SYSTEMS/PC'' and ''COMPONENTS''
-  * Turn off the two main switches: **SYSTEM/PC** and **COMPONENTS**
+  * **Wait 5 min. for Ar laser to cool down**
+  * Turn off the ''MAIN SWITCH''
-===== If you want to use the UV laser ablation system: =====
-  * Please contact the [[about|Bioimaging Facility]] (Ext: 47316). The ablation system requires specific training even for users that are already trained on the LSM 7 Live itself.
 ----
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