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--- zeiss_lsm_880 [2016/04/04 16:43]
bioimaging
+++ zeiss_lsm_880 [2016/04/05 00:28]
bioimaging [Microscope overview]
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-&rarr; <a href="https://imm.medicina.ulisboa.pt/intranet/booking/resource_admin.php?resourcepage=0&rid=128"><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png">  Zeiss LSM 880 Booking</a>
+&rarr; <a href="https://imm.medicina.ulisboa.pt/intranet/booking/month.php?user=ZEISSLSM8&rid=128"><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png">  Zeiss LSM 880 Booking</a>
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 &rarr; <a href="/facility/bioimaging/doku.php?id=zeiss_lsm_880_quality"><img src="/facility/bioimaging/lib/exe/fetch.php?media=list-icon.png" width=18> Zeiss LSM 880 Quality Control</a>
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 ===== Microscope overview =====
-The Zeiss LSM 7 Live //confocal line-scanning microscope// is a fast imaging system able to analyze high-speed processes with a time resolution of just a few microseconds. It is an upright microscope equipped with water dipping objectives specially suited for live samples in aqueous media. The scanning unit uses a laser beam with a rectangular cross-section to [[http://www.nature.com/nmeth/journal/v3/n11/thumbs/nmeth971-F2.jpg|illuminate a whole line in the sample]], instead of a single point. This makes image acquisition by its array CCD detectors faster than conventional PMT light detection but it also lowers the Z-resolution due to the usage of slits instead of conventional pinholes. Note that the system is equipped with two lasers (488 and 561 nm) but dual color imaging is not advised since there are no bandpass filters in the system (channel crosstalk is likely to occur). A UV laser controlled by galvanometric mirrors is available for precise ablation in user-defined regions of interest. The spatial resolution of this system is not as high as a //point-scanning confocal system// such as the [[zeiss_lsm_880|Zeiss LSM 880]] or [[zeiss_lsm_710|Zeiss LSM 710]] but it is much faster than these two systems, achieving scanning speeds which may be even higher than the [[3i_marianas_sdc|3i Marianas SDC]] spinning disk if the acquisition is performed only in a few lines. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]].
+The Zeiss LSM 880 with Airyscan is a //confocal point-scanning microscope// able to generate high-resolution three-dimensional images of thick specimens with high sensitivity and low photodamage. It is an inverted microscope specially suitable for live cell imaging and photobleaching experiments, equipped with a large size incubator for temperature control and CO2 supply. Its [[http://www.zeiss.com/content/dam/Microscopy/Products/laser-scan/LSM%20880/zeiss-lsm880-airyscan-principle-672.jpg|Airyscan]] 32 channel area detector is able to collect all light from an Airy pattern simultaneously, allowing for increased resolution (140 nm laterally and 400 nm axially, at 488 nm). Its scanning unit further includes two PMT detectors (one of them cooled) as well as a GaAsP detector for increased sensitivity (45% QE compared to ~25% QE for conventional PMT).  It is equipped with lasers from violet to far red (405, 458, 488, 514, 561, 594 and 633 nm excitation wavelengths). With this system you can perform optical sectioning of fluorescent samples which are too thick for a widefield system such as the [[zeiss_cell_observer_wf|Zeiss Cell Observer WF]] or the [[zeiss_axiovert_200m|Zeiss Axiovert 200M]]. Image resolution with Airyscan is higher than the [[zeiss_lsm_710|Zeiss LSM 710]] and detection sensitivity is also higher than faster confocal systems such as the spinning disk [[3i_marianas_sdc|3i Marianas SDC]] or the //line scanning// [[zeiss_lsm_7_live|Zeiss LSM 7 Live]]. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]].
-{{info.gif?|}} **If you are going to use the 561 nm laser please write '561' in the Event Field name**
 ===== System components =====

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