zeiss_cell_observer
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zeiss_cell_observer [2024/01/28 12:14] bioimaging [Microscope overview] |
zeiss_cell_observer [2024/04/01 23:50] (current) bioimaging [Microscope overview] |
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| ===== Microscope overview ===== | ===== Microscope overview ===== | ||
| - | {{ :widefield_lightpath.png?0x300}}The Zeiss Cell Observer is a fully motorized inverted //widefield fluorescence microscope// ideal for live-cell imaging with fast acquisition speeds. It is equipped with a large cage incubator and a stage top incubator for temperature control and CO2 supply and with Definite Focus for hardware focus control during long time-lapse acquisitions. Using the ZEN software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. The stage is furthermore equipped with a piezo for fast Z displacement. You can choose between a HXP metal halide lamp or fast-switching LEDs for illumination. Its sensitive cooled Axiocam 506m camera allows you to capture weak fluorescent signals and minimize photobleaching/photodamage in light sensitive samples. | + | {{ :widefield_lightpath.png?0x300}}The Zeiss Cell Observer is a fully motorized inverted //widefield fluorescence microscope// ideal for live-cell imaging with fast acquisition speeds. It is equipped with a large cage incubator and a stage top incubator for temperature control and CO2 supply and with Definite Focus for hardware focus control during long time-lapse acquisitions. Using the ZEN software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. The stage is furthermore equipped with a piezo for fast Z displacement. You can choose between a HXP metal halide lamp or fast-switching LEDs for illumination. Its cooled [[https://asset-downloads.zeiss.com/catalogs/download/mic/a8e5b4d7-25f6-4069-8f03-c6713d044c87/EN_product-info_Axiocam-506_mono.pdf|Axiocam 506m]] camera allows you to capture weak fluorescent signals and minimize photobleaching/photodamage in light sensitive samples. It is also equipped with a high sensitivity [[https://www.photometrics.com/products/prime-family/primebsiexpress|Photometrics Prime BSI Express]] sCMOS camera for high resolution imaging with maximized signal detection. |
| - | * **Microscope**: Zeiss Axio Observer | + | * **Microscope**: [[https://www.zeiss.com/microscopy/en/products/light-microscopes/widefield-microscopes/axio-observer-for-life-science-research.html|Zeiss Axio Observer]] |
| * **LED Illumination**: [[https://www.zeiss.com/microscopy/en/products/accessories/colibri.html|Zeiss Colibri 7]] | * **LED Illumination**: [[https://www.zeiss.com/microscopy/en/products/accessories/colibri.html|Zeiss Colibri 7]] | ||
| - | * **Camera**: [[https://www.zeiss.com/microscopy/int/products/microscope-cameras/axiocam-506-mono.html|Axiocam 506 mono CCD]] | + | * **Camera** (left port): [[https://asset-downloads.zeiss.com/catalogs/download/mic/a8e5b4d7-25f6-4069-8f03-c6713d044c87/EN_product-info_Axiocam-506_mono.pdf|Axiocam 506 mono CCD]] |
| + | * **Camera** (right port): [[https://www.photometrics.com/products/prime-family/primebsiexpress|Photometrics Prime BSI Express sCMOS]] | ||
| + | |||
| + | {{::phenocycler.png?260 |}}This system is also set up to work together with the [[https://www.youtube.com/watch?v=OQGJX45Wsgw|AKOYA PhenoCycler]] fluidics instrument for automated iterative imaging cycles of whole slide tissues for [[https://www.akoyabio.com/why-spatial-biology/|Spatial Biology]] applications, such as image-based quantification of biomarkers to predict immunotherapy response, or quantification of cells by lineage and variant with single-cell resolution in the context of an intact tissue sample. | ||
| + | |||
| + | * [[https://www.youtube.com/watch?v=OQGJX45Wsgw|Understanding the PhenoCycler Technology]] | ||
| + | * [[https://www.akoyabio.com/webinar/a-beginners-guide-to-codex/|A Beginner’s Guide to CODEX]] | ||
| + | * [[https://www.akoyabio.com/webinar/spatial-phenotyping-a-revolutionary-approach-to-biomarker-discovery-for-cancer-immunotherapy/|Spatial Phenotyping: A Revolutionary Approach to Biomarker Discovery for Cancer Immunotherapy]] | ||
| {{:warning.png?25|}} Data files older than **15 days** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. | {{:warning.png?25|}} Data files older than **15 days** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. | ||
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| * Turn on the temperature and CO2 controller - white power strip (if you need them). | * Turn on the temperature and CO2 controller - white power strip (if you need them). | ||
| * If you need CO2, open the Cell Observer CO2 valve | * If you need CO2, open the Cell Observer CO2 valve | ||
| - | * Turn on the motorized stage controller (**A** on remote control) | + | * Turn on the motorized stage controller (**1** on remote control) |
| {{zeiss_axio_observer_a.jpg?height=150|}} | {{zeiss_axio_observer_a.jpg?height=150|}} | ||
| - | * Turn on the microscope power supply (**B** on remote control) | + | * Turn on the microscope power supply (**2** on remote control) |
| {{zeiss_axio_observer_b.jpg?height=150|}} | {{zeiss_axio_observer_b.jpg?height=150|}} | ||
| * Turn on the computer | * Turn on the computer | ||
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| {{::workspace_selection.png?250|}} | {{::workspace_selection.png?250|}} | ||
| - | ===== Microscope Turn Off procedures ===== | + | ===== System Turn Off procedures ===== |
| If there is another user for this microscope is the __next hour__: | If there is another user for this microscope is the __next hour__: | ||
| * Leave the fluorescent light source on | * Leave the fluorescent light source on | ||
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| {{:cell_observer_pc_vs_ups.png?250|}} | {{:cell_observer_pc_vs_ups.png?250|}} | ||
| * Clean up immersion objectives | * Clean up immersion objectives | ||
| - | * Turn off the microscope power supply (**B** on remote control) | + | * Turn off the microscope power supply (**2** on remote control) |
| - | * Turn off the motorized stage controller (**A** on remote control) | + | * Turn off the motorized stage controller (**1** on remote control) |
| * Turn off the Temperature and CO2 controller - white power strip (if used) | * Turn off the Temperature and CO2 controller - white power strip (if used) | ||
| * Close the Cell Observer CO2 valve (if used) | * Close the Cell Observer CO2 valve (if used) | ||
zeiss_cell_observer.1706440456.txt.gz · Last modified: 2024/01/28 12:14 by bioimaging
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