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--- nikon_eclipse_ti [2022/09/01 11:55]
bioimaging
+++ nikon_eclipse_ti [2024/02/12 22:47] (current)
bioimaging
@@ Line 10: / Line 10: @@
 <b>Model</b>: Eclipse Ti<br>
 <b>Nickname</b>: "006"<br>
-<b>Software</b>: NIS-Elements AR 5.11.01<br>
+<b>Software</b>: NIS-Elements AR 5.42.05<br>
 <b>Year</b>: 2010 <br>
 <b>SN</b>: 602805 <br>
 &nbsp <br>
+Data will be deleted after: <b>3 months</b>
 &nbsp <br>
 &nbsp <br>
@@ Line 27: / Line 28: @@
 ===== Microscope overview =====
-{{  :widefield_lightpath.png?0x300}}The Nikon Eclipse Ti is a fully motorized inverted //widefield fluorescence microscope// ideal for live-cell imaging applications. It is equipped with a small stage incubator for temperature control and CO2 supply and with Perfect Focus System for hardware focus control during long time-lapse acquisitions. Using either Metamorph or Nikon Elements software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. It is equipped with a Andor Neo 5.5 monochrome cooled sCMOS camera ideal for time-lapse and tile-and-stitch microscopy. You can perform Z-stack acquisition and use [[https://huygens.i3s.up.pt/login.php|PPBI Huygens RM]] [[http://micro.magnet.fsu.edu/primer/digitalimaging/deconvolution/deconvolutionhome.html|deconvolution]] server to significantly improve image resolution even in thick samples. If don't want to use deconvolution, check out one of the confocal systems such as the point scanners [[zeiss_lsm_710|Zeiss LSM 710]] and [[zeiss_lsm_880|Zeiss LSM 880]] or spinning disks [[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]]. If your personal computer cannot handle all the data you collected, check out the [[colossus|Colossus]].
+{{  :widefield_lightpath.png?0x300}}The Nikon Eclipse Ti is a fully motorized inverted //widefield fluorescence microscope// ideal for live-cell imaging applications. It is equipped with a small stage incubator for temperature control and CO2 supply, and with Perfect Focus System for hardware focus control during long time-lapse acquisitions. Using NIS-Elements software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. It is equipped with an Andor Neo 5.5 monochrome cooled sCMOS camera.
   * **Microscope**: [[https://www.microscope.healthcare.nikon.com/en_EU/products/inverted-microscopes/eclipse-ti-series|Nikon Eclipse Ti]]
   * **Camera**: [[https://andor.oxinst.com/products/scmos-camera-series/neo-5-5-scmos|Andor Neo 5.5 sCMOS]]
+This microscope is also equipped with a [[https://www.ionoptix.com/products/systems/calcium-and-contractility-system/|Ionoptix Calcium and Contractility system]] for high-speed simultaneous acquisition of Fura-2 fluorescence with video-based cell length measurement to study myocyte function, contracting spontaneously or with field stimulation.
+{{::ionoptix_realtime-acquisition-cell-length-600x500.jpg?200  |}}
+  * **High-speed contractility camera**: [[https://www.ionoptix.com/products/components/sensors/myocam-s3/|MyoCam-S3]]
+  * **Electrical Field Stimulator**: [[https://www.ionoptix.com/products/components/stimulators/myopacer-cell-stimulator/|MyoPacer]]
+  * **LED Light Source**: [[https://www.ionoptix.com/products/components/light-sources/led-light-source/|UV LEDs for Fura-2 excitation]]
+  * **High-speed PMT**: [[https://www.ionoptix.com/products/components/sensors/pmt-sub-system/|PMT400]] for photon counting at 1kHz
+  * **Software**: [[https://www.ionoptix.com/products/software/ionwizard-core-and-analysis/|IonWizard]] 7.7.3.180
 {{:warning.png?25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link.
@@ Line 36: / Line 46: @@
 ===== System components =====
-==== Filtersets ====
+==== Filtersets Turret 1 (top) ====
 ^  Position  ^  Filterset  ^  Reference  ^  Excitation  ^  Dichroic  ^  Emission  ^
@@ Line 45: / Line 55: @@
 |  5  |  Cy5  |  2SK4CY50  |  608-648 nm  |  660 nm  |  672-712 nm  |
 |  6  |  Empty  |||||
+==== Filtersets Turret 2 (bottom) ====
+^  Position  ^  Filterset  ^
+|  1  |  Empty  |
+|  2  |  Empty  |
+|  3  |  Empty  |
+|  4  |  Empty  |
+|  5  |  Empty  |
+|  6  |  Fura-2  |
 ==== Objectives ====
@@ Line 50: / Line 70: @@
 |  10x  |  CFI Plan Fluor DLL Ph1  |  Air  |  0.30  |  16.00  |   [[https://www.microscope.healthcare.nikon.com/en_EU/selectors/objective-comparison/-1835|MRH10101]]  |
 |  20x  |  CFI S Plan Fluor ELWD ADM Ph1  |  Air  |  0.45  |  6.90 - 8.20  |  [[https://www.microscope.healthcare.nikon.com/en_EU/selectors/objective-comparison/-1813|MRH48230]]  |
+|  40x  |  CFI CFI Plan Apochromat Lambda D  |  Air  |  0.95  |  0.21  |  [[https://www.microscope.healthcare.nikon.com/products/optics/selector/comparison/-179806|MRH70470]]  |
 |  40x  |  CFI S Plan Fluor ELWD ADM Ph2  |  Air  |  0.60  |  2.80 - 3.60  |  [[https://www.microscope.healthcare.nikon.com/en_EU/selectors/objective-comparison/-1814|MRH48430]]  |
-|  40x  |  Plan Apo DM Ph3  |  Oil  |  1.00  |  0.16  |  |
 |  60x  |  Plan Apo DM Ph3  |  Oil  |  1.40  |  0.13  |  |
 |  100x  |  Plan Apo DM Ph3  |  Oil  |  1.40  |  0.13  |  |

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