procuramos
perguntas:

pt en
Menu

Bernardes, Gonçalo J. L.
Lab

Biologia Química e Biotecnologia Farmacêutica

O programa do meu grupo de investigação situa-se na interface entre a Química e a Biologia. Este programa centra-se no desenvolvimento de novos métodos que permitam a modificação de proteínas e ligandos de um modo específico e cujas condições sejam compatíveis com células e animais. Estes métodos serão a base para esclarecer importantes mecanismos biológicos, e criar uma nova geração de conjugados para o tratamento de cancro e para vacinação.

Modificação selectiva de proteínas: No centro do nosso programa de investigação está o desenvolvimento de métodos quimio-selectivos eficientes, compatíveis com sistemas biológicos e a sua utilização para redesenhar a estrutura e função das proteínas de interesse biológico e terapêutico.

Biologicos sintéticos: as proteínas modificadas são uma grande promessa para o tratamento e prevenção de doenças humanas. O nosso grupo de investigação está particularmente interessado na entrega específica de moléculas tóxicas em células cancerígenas e na construção de glicoproteínas como potenciais vacinas.

Entrega terapêutica de CO: Moléculas libertadoras de monóxido de carbono (CO-RMs) têm o potencial para constituir tratamentos seguros se a libertação CO in vivo ocorrer de forma controlada espacial e temporalmente. Estamos actualmente a investigar o uso de metaloproteínas de carbonilo para a entrega de quantidades terapêuticas de CO em tecidos doentes.

  • Áreas de Investigação

    • Modificação selectiva de proteínas
    • Biológicos sintéticos
    • Terapias selectivas para o tratamento de cancro
    • Glicoproteínas sintéticas para vacinação
  • Equipa de Investigação

    • Projectos de Investigação em Curso

      • 2014/2017 Pesquisador Visitante Especial, CNPq, Brazil
      • 2014/2017 Marie Curie CIG
      • 2014/2018 Hovione Grant
      • 2013/2018 Investigador FCT - Fundação para a Ciência e a Tecnologia, Portugal
      • 2015/2019 ProteinConjugates H2020-MSCA-ITN
      • 2015/2019 GLICOVAX H2020-MSCA-ITN
      • 2016/2019 PTDC/BBB-BSS/0827/2014 
      • 2016/2019 PTDC/QEQ-QOR/1434/2014
    • Prémios

      • 2016 Harrison-Meldola Memorial Prize – Royal Society of Chemistry
      • 2016 Chem Soc Rev Emerging Investigator Lectureship 2016
      • 2016 International Award of the Belgian Society of Pharmaceutical Sciences (BSPS) 2016
      • 2016 RSC-SAFEA Visiting Researcher Programme – China 2016
      • 2015 2nd Prize – Royal Society of Chemistry Emerging Technologies Competition 2015 – Health Care
      • 2015 European Prize of the RSEQ Chemical Biology Division
      • 2014 European Young Chemist Award (EYCA2014) - Silver Medal
      • 2014 Finalist of the RSC Emerging Technologies Competition 2014
      • 2013 Royal Society of Chemistry/BMOS Young Investigator Award
      • 2013 EFMC Prize for a Young Medicinal Chemist in Academia
      • 2013 Medalha de Serviços Distintos Grau Prata do Ministério da Saúde
      • 2013 Selected for The Lindau Nobel Laureate Meeting
      • 2013 Journals Grant for International Authors, Royal Society of Chemistry
      • 2012 Keystone Symposia Angiogenesis Scholarship
      • 2011 JSP Fellowship of the 46th Bürgenstock Meeting, Brunnen (Switzerland)
      • 2010 OzBio2010 Young Scientist Fellowship, Biochemical Society (UK)
      • 2008 Honorable Mention – European Young Chemistry Award
      • 2007 Poster Highlight Presentation, Gordon Conference Carbohydrates
      • 2006 First Prize in the Pfizer Oxford Organic Chemistry Poster Symposium
      • 2006 Silver Medal for Merit from the Town Hall of Torres Vedras, Portugal
      • 2006 EPA Cephalosporin Scholarship, Linacre College, University of Oxford
    • Publicações Seleccionadas

      56. (-)-Englerin A is a L-type calcium channel modulator
      Rodrigues T; Sieglitz F; Somovilla VJ; Cal PMSD; Galione A; Corzana F; Bernardes GJL*
      Angew. Chem. Int. Ed. 2016, DOI: 10.1002/anie.201604336

      55.  Iminoboronates are efficient intermediates for selective, rapid and reversible N-terminal cysteine functionalisation
      Faustino H; Silva MJSA; Veiros LF; Bernardes GJL; Gois PMP
      Chem. Sci. 2016, DOI: 10.1039/C6SC01520D

      54. Natural product modulators of transient receptor potential (TRP) channels as potential anti-cancer agents
      Rodrigues T; Sieglitz F; Bernardes GJL*
      Chem. Soc. Rev. 2016, DOI: 10.1039/C5CS00916B
      Emerging Investigators Issue 2016.

      53. Construction of Homogeneous Antibody-drug Conjugates using Site-selective Protein Chemistry
      Akkapeddi P; Azizi S-A; Freedy A; Cal PMSD; Gois PMP; Bernardes GJL*
      Chem. Sci. 2016, 7, 2954-2963

      52. Urban endocrine disruptors targeting breast cancer proteins
      Montes-Grajales D; Bernardes GJL*; Olivero-Verbel JT
      Chem. Res. Toxicol. 2016, 29, 150–161

      51. Site-selective protein-modification chemistry for basic biology and drug development
      Krall N; da Cruz FP; Boutureira O; Bernardes GJL*
      Nature Chem2016, 8, 103–113
      Highlighted in Nature Chem 20168, 91 - A little less conjugation, a little more accuracy.

      50. Sulfur-Limonene Polysulfide: A Material Synthesized Entirely from Industrial Waste and Its Use in Removing Toxic Metals from Water and Soil
      Crockett MP; Evans AM; Worthington MJH; Albuquerque IS; Slattery AD; Gibson CT; Bernardes GJL, Chalker JM
      Angew. Chem. Int. Ed.2016, 55, 1714–1718
      Selected as a Hot Paper.

      Highlighted in Chemical&Engineering News, November 2, 2015.

      49. A N-acetyl cysteine ruthenium tricarbonyl conjugate enables simultaneous release of CO and ablation of ROS species
      Seixas JD; Chaves-Ferreira M; Montes-Grajales D; Gonçalves AM; Marques AR; Saraiva LM; Olivero-Verbel J; Romão CC; Bernardes GJL*
      Chem Eur J 2015, Accepted for publication.
      Selected as a Very Important Paper (VIP).

      48. Peptide anchor for folate-targeted liposomal delivery 
      Nogueira E; Mangialavori IC; Loureiro A; Azoia NG; Sárria MP; Nogueira P; Freitas J; Härmark J; Shimanovich U; Rollett A; Lacroix G; Bernardes GJL, Guebitz GM; Herbert H; Moreira A; Carmo AM; Rossi JPFC; Gomes AC; Preto A; Cavaco-Paulo A 
      Biomacromolecules 2015, DOI:10.1021/acs.biomac.5b00823.

      47. Size Controlled Protein Nanoemulsions for Active Targeting of Folate Receptor Positive Cells

      Loureiro A; Nogueira E; Azoia NG; Sárria MP; Abreu AS; Shimanovich U; Rollet A; Härmark J; Hebert H; Guebitz G;Bernardes GJL; Preto A; Gomes AC; Cavaco-Paulo A
      Colloids Surf B Biointerfaces 2015135, 90-98.

      46. Synthesis of Fluorosugar Reagents for the Construction of Well-Defined Fluoroglycoproteins

      Salvadó M; Amgarten B; Castillón S; Bernardes GJL*; Boutureira O 
      Org Lett 201517, 2836-2839.

      45. X-ray Structures decipher the Non-equivalence of Serine and Threonine O-glycosylation points: Implications for the Molecular Recognition of the Tn Antigen by an anti-MUC1 Antibody
      Martínez-Sáez N; Castro-López J; Valero-González J, Madariaga D; Compañón I; Somovilla VJ; Salvadó M; Asensio JL; Jiménez-Barbero J; Avenoza A; Busto JH; Bernardes GJL; Peregrina JM; Hurtado-Guerrero R; Corzana F
      Angew Chem Int Ed 2015, DOI: 10.1002/anie.201502813R1.

      44. Dynamic interplay between catalytic and lectin domains of GalNAc-transferases modulates protein O-glycosylation 
      Lira-Navarrete E; de las Rivas M; Compañón I; Pallarés MC; Kong Y; Iglesias-Fernández J; Bernardes GJL; Peregrina JM; Rovira C; Bernadó P; Bruscolini P; Clausen H; Lostao A; Corzana F; Hurtado-Guerrero R 

      Nat Commun 20156, 6937.

      43. Enhancing methotrexate tolerance with folate tagged liposomes in arthritic mice
      Nogueira E; Lager F; Le Roux D; Nogueira P; Freitas J; Charvet C; Renault G; Loureiro A; Almeida CR; Ohradanova-Repic A; Machacek C; Bernardes GJL; Moreira A; Stockinger H; Burnet M; Carmo AM; Gomes AC; Preto A; Bismuth G; Cavaco-Paulo A 
      J Biomed Nanotechnol 201511, 2243-2252.

      42. Folic Acid-Tagged Protein Nanoemulsions Loaded with CORM-2 Enhance the Survival of Mice Bearing Subcutaneous A20 Lymphoma Tumors 
      Loureiro A; Bernardes GJL*; Shimanovich U; Sárria MP; Nogueira E; Preto A; Gomes AC; Cavaco-Paulo A 
      Nanomedicine 201511, 1077-1183.

      41. Histidine-selective metallation in Interleukin-8 with a ruthenium dicarbonyl fragment 
      Albuquerque IS; Jeremias HF; Chaves-Ferreira M; Matak-Vinkovic D; Boutureira O; Romão CC; Bernardes GJL* 
      Chem Commun 201551, 3993-3996.

      40. Advances in chemical protein modification
       

      Boutureira O; Bernardes GJL* 
      Chemical Reviews 2015115, 2174–2195.

      39. Spontaneous CO Release from RuII(CO)2-Protein Complexes in Aqueous Solution, Cells and Mice
      Ferreira MC; Albuquerque IS; Matak-Vinkovic D; Coelho AC; Carvalho SM; Saraiva LM; Romão CC; Bernardes GJL*
      Angew Chem Int Ed 201554, 1172-1175.

      38. Collagen labelling with an azide-proline chemical reporter in live cells 
      Amgarten B; Rajan R; Martínez-Sáez N; Oliveira BL; Albuquerque IS; Reid DG; Brooks RA; Duer MJ; Bernardes GJL*
      Chem Commun 201551, 5250-5252
      This manuscript is part of the Emerging Investigators Issue 2015. 

      37. A contribution to the rational design of Ru(CO)3Cl2L complexes for in vivo delivery of CO 
      Seixas JD; Santos MFA; Mukhopadhyay A; Coelho AC; Reis PM; Veiros LF; Marques AR; Penacho N; Gonçalves AML; Romão MJ; Bernardes GJL; Santos-Silva, T; Romão CC 
      Dalton Trans. 201544, 5058-5075.

      36. Functionalized protein nanoemulsions by incorporation of chemically modified BSA
      Loureiro A; Abreu AS; Sárria MP; Figueiredo MCO; Saraiva LM; Bernardes GJL; Gomes A; Cavaco-Paulo A

      RSC Adv. 20155, 4976-4983.

      35. Highlights from the 49th EUCHEM conference on stereochemistry, Bürgenstock, Switzerland, May 2014
      Bernardes GJL*; Lawrence AL 

      Chem. Commun. 201450, 10752-10757.

      34. Cysteine Selective Reactions for Antibody Conjugation
      Cal PMSD; Bernardes GJL*; Gois PMP 

      Angew. Chem. Int. Ed. 201453, 10585-10587.

      33. Carbon-monoxide releasing molecules for the delivery of therapeutic CO in vivo
      García-Gallego, S.; Bernardes, G.J.L.* 
      Angew. Chem. Int. Ed. 201453, 9712-9721.

      32. A Small Molecule Drug Conjugate for the Treatment of Carbonic Anhydrase IX Expressing Tumors
      Krall N; Pretto F; Decurtins W; Bernardes GJL; Supuran CT; Neri D 

      Angew. Chem. Int. Ed. 201453, 4231-4235.

      31. Curative Properties of Non-Internalizing Antibody-Drug Conjugates Based on Maytansinoids
      Perrino E; Steiner M; Krall N; Bernardes GJL; Pretto F; Casi G; Neri D

      Cancer Research 201474, 2569-2578.

      30. Rationally Designed Short Polyisoprenol-linked PglB Substrates for Engineered Polypeptide N-Glycosylation
      Liu F; Vijayakrishnan B; Faridmoayer A; Taylor TA; Parsons TB; Bernardes GJL; Kowarik M; Davis BG 
      J. Am. Chem. Soc. 201415, 566-569.

      29. Protein Micro and Nano Capsules for Biomedical Applications 
      Shimanovich U; Bernardes GJL*, Knowles TJ; Cavaco-Paulo A 
      Chem. Soc. Rev. 201443, 1361-1371.

      28. Site-specific Chemical Modification of Antibody Fragments with Traceless Cleavable Linkers
      Bernardes GJL; Steiner M; Hartmann I; Neri D; Casi G 
      Nature Protocols 20138, 2079-2089.

      27. Highlights from the 48th EUCHEM conference on stereochemistry, Bürgenstock, Switzerland, May 2013
      Bernardes GJL*
      Chem. Commun. 2013, 49, 8578-8582.

      26. Synthetically Defined Glycoprotein Vaccines: Current Status & Future Directions
      Adamo D; Nilo A; Castagner B; Boutureira O; Berti F; Bernardes GJL*
      Chemical Science 20134, 2995-3008.

      25. Spacer Length Shapes Drug Release and Therapeutic Efficacy of Traceless Disulfide-linked ADCs Targeting the Tumor Neovasculature
      Steiner M; Hartmann I; Perrino E; Casi G; Brighton S; Jelesarov I; Bernardes GJL*; Neri D
      Chemical Science 20134, 297-302.

      24. Generation of Liver Specific Carbon Monoxide-Releasing Molecule (CO-RM) as Drug Candidates for the Treatment of Acute Liver Injury: Targeting of CO-RMs to the Liver
      Marques AR; Kromer L; Gallo DJ; Penacho NM; Rodrigues SS; Seixas JD; Bernardes GJL; Reis PM; Otterbein SL; Ruggieri RA; Gonçalves ASG; Gonçalves AML; De Matos MN; Bento I; Otterbein LE; Blättler WA; Romão CC
      Organometallics 201231, 5810-5822.
      This article is part of the Organometallics in Biology and Medicine special issue.

      23. Fucose-specific Conjugation of Hydrazide Derivatives to a Vascular-targeting Monoclonal Antibody in IgG Format
      Zuberbühler K; Casi G; Bernardes GJL; Neri D
      Chem. Commun. 201248, 7100-7102.

      22. Developing Drug Molecules for Therapy with Carbon Monoxide
      Romão CC; Seixas JD; Blättler WA; Bernardes GJL*
      Chem. Soc. Rev. 201241, 3571-3583.

      21. A Novel CO-releasing Molecule Fully Protects Mice from Severe Malaria
      Pena, A.C.; Penacho, N.; Mancio da Silva, L.; Neres, R.; Seixas, J.D.; Fernandes, A.C.; Romão, C.C.; Mota, M.M.;Bernardes, G.J.L.*; Pamplona, A.
      Antimicrob. Agents Chemother. 201256, 1281-1290. 

      20. A Traceless Vascular Targeting Antibody-Drug Conjugate for Cancer Therapy
      Bernardes GJL; Casi G; Trüssel S; Hartmann I; Schwager K; Scheuermann J; Neri D
      Angew. Chem. Int. Ed. 201251, 941-944.
      Selected as a Very Important Paper (VIP).
      Article highlighted in Chimia 201266, 130; ETH Life – “Starving Cancer”

      19. SeS-linked Homogeneous Glycopeptides and Glycoproteins: Synthesis of Human Hepatic ‘Se-Metabolite A'
      Boutureira O; Bernardes GJL; Fernández-González M; Davis BG
      Angew. Chem. Int. Ed. 201251, 1432-1436.

      18. Direct Radiolabelling of Proteins at Cysteine using [18F]-fluorosugars
      Boutureira O; Bernardes GJL; D’Hooge F; Davis BG
      Chem. Commun. 201130, 10010-10012.

      17. Towards Improved Therapeutic CORMs: Understanting the Reactivity of CORM-3 with Proteins
      Santos-Silva T; Mukhopadkyay A; Seixas JD; Bernardes GJL; Romão CR; Romão MJ 
      Curr. Med. Chem. 201118, 3361-3366.

      16. Methods for the Conversion of Cysteine to Dehydroalanine on Peptides and Proteins
      Chalker JM; Gunnoo SB; Boutureira O; Gerstberger SC; Fernández-González M; Bernardes GJL; Griffin L; Hailu H; Schofield CJ; Davis BG
      Chemical Science 20112, 1666-1676.
      Article featured on a Cover and highlighted in the Chemical Science Blog.

      15. A “Tag-and-Modify” Approach to Site-selective Protein Modification
      Chalker JM; Bernardes GJL; Davis BG
      Acc. Chem. Res. 201144, 730-741.
      This article is part of the Bioorthogonal Chemistry in Biology special issue.

      14. Site-selective Traceless Staudinger Ligation for Glycoprotein Synthesis Reveals Scope and Limitations
      Bernardes GJL; Linderoth L; Doores KJ; Boutureira O; Davis BG
      ChemBioChem 201112, 1383-1386.
      Most accessed articles from ChemBioChem in May, June and July 2011.

      13. A Coordinated Synthesis and Conjugation Strategy for the Preparation of Homogeneous Glycoconjugate Vaccine Candidates
      Grayson EJ; Bernardes GJL; Chalker JM; Boutureira O; Koeppe JR; Davis BG
      Angew. Chem. Int. Ed. 201150, 4127-4132.

      12. CORM-3 Reactivity Towards Proteins: The Crystal Structure of a Ru(II) Dicarbonyl-Lysosyme Complex
      Santos-Silva T; Mukhopadkyay A; Seixas JD; Bernardes GJL*; Romão CR; Romão MJ
      J. Am. Chem. Soc. 2011133, 1192-1195. 

      11. Site-selective Chemoenzymatic Construction of Synthetic Glycoproteins using Endoglycosidases
      Fernández-González M; Boutureira O; Bernardes GJL; Chalker JM; Young MA; Errey JC; Davis BG
      Chemical Science 20101, 709-715.

      10. Design, Synthesis and Biological Evaluation of Carbohydrate-functionalized Cyclodextrins and Liposomes for Hepatocyte-specific Targeting
      Bernardes GJL; Kikkeri R; Maglinao M; Laurino P; Collot M; Hong SY; Lepenies B; Seeberger PH
      Org. Biomol. Chem. 20108, 4987-4996.
      Article highlighted as Hot Article.

      9. Fluoroglycoproteins: Ready Chemical Site-selective Incorporation of Fluorosugars into Proteins
      Boutureira O; D’Hooge F; Fernández-González M; Bernardes GJL; Sánchez-Navarro M; Koeppe JR; Davis BG
      Chem. Commun. 201046, 8142-8144.
      Article highlighted as Hot Article and featured on a Cover.

      8. Combined Approaches to the Synthesis and Study of Glycoproteins
      Bernardes GJL; Castagner B; Seeberger PH
      ACS Chem. Biol. 20094, 703-713.
      Most accessed articles from ACS Chemical Biology in 2009.

      7. Chemical Modification of Proteins at Cysteine: Opportunities in Chemistry and Biology
      Chalker JM; Bernardes GJL; Lin AY; Davis BG
      Chem. Asian J. 20094, 630-640. 
      Most accessed articles from Chemistry: An Asian Journal in 2009 and 2010.

      6. Allyl Sulfides are Priviliged Substrates in aqueous Olefin Cross-Metathesis: Application to Site-selective Protein Modification
      Lin AY; Chalker JM; Floyd N; Bernardes GJL; Davis BG 
      J. Am. Chem. Soc. 2008130, 9642-9643. 
      Article highlighted in:
      Chemical&Engineering News, July 14, 2008
      Nature Chemical Biology 20084, 527-528
      Faculty of 1000 Biology

      5. Chemical Site-Selective Prenylation of Proteins
      Gamblin DP; van Kasteren SI; Bernardes GJL; Chalker JM; Oldham NJ; Fairbanks AJ; Davis BG
      Mol. BioSyst. 20084, 558-561.

      4. Facile Conversion of Cysteine and AlkylCysteines to Dehydroalanine: Versatile and Switchable Access to Functionalized Proteins
      Bernardes GJL; Chalker JM; Errey JC; Davis BG
      J. Am. Chem. Soc. 2008130, 5052-5053.
      Article highlighted in:
      Nature Chemical Biology 20084, 527-528 
      Chemical&Engineering News, March 31, 2008

      3. From Disulfide- to Thioether-linked Glycocoproteins
      Bernardes GJL; Grayson EJ; Thompson S; Chalker JM; Errey JC; Oualid FE; Claridge TDW; Davis BG
      Angew. Chem. Int. Ed. 200847, 2244-2247. 

      2. A Trisulfide-linked Glycoprotein
      Bernardes GJL; Marston JP; Batsanov AS; Howard JAK; Davis BG
      Chem. Commun. 2007, 3145-3147.

      1. The Direct Formation of Glycosyl Thiols from Reducing Sugars Allows One-pot Protein Glycoconjugation
      Bernardes GJL; Gamblin DP; Davis BG
      Angew. Chem. Int. Ed. 200645, 4007-4011.

    Este site usa cookies. Algumas cookies são essenciais para a correcta operação do mesmo e já foram configuradas.
    Pode bloquear e apagar as cookies deste site, mas algumas partes poderão não funcionar correctamente.