https://imm.medicina.ulisboa.pt/facility/flowcytometry/ 2026-04-15T02:01:18+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/ https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=favicon.ico text/html 2026-04-13T20:12:08+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=about&rev=1776103928&do=diff About the Flow Cytometry Platform The Flow Cytometry Platform provides a state of the art flow cytometry service and expertise to GIMM researchers as well as external groups from other research institutes and companies. Laboratory staff is well trained in instrument operation, providing support to cell sorting, sample acquisition, panel design and data analysis. Training is available for all analytical instruments and SOP are implemented to comply with the highest quality standards requ… text/html 2025-07-23T00:02:29+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=acknowledge&rev=1753221749&do=diff How to Acknowledge us If you used our services and equipment, please acknowledge us on your publications or any other formal presentations. This is one of our key performance indicators and very important for future funding. You are welcome to use the following examples: text/html 2024-02-29T17:07:23+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=amnis_imagestreamx_usage&rev=1709222843&do=diff Amnis ImageStreamX Usage Statistics ImageStreamX Click here to get info on the Amnis ImageStreamX ---------- Back to Statistics text/html 2026-04-13T11:46:07+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=aria_fusion_contamination&rev=1776073567&do=diff BD FACSAria Fusion Pseudomonas contamination 2025 Date Sheath tank Stream 03/01 ✘ ✔ 10/01 ✘ ✔ 17/01 ✘ ✔ 24/01 ✘ ✔ 31/01 ✘ ✔ 07/02 ✔ ✔ text/html 2026-04-13T11:45:42+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=aria_iii_contamination&rev=1776073542&do=diff BD FACSAria III Pseudomonas contamination 2025 Date Sheath tank Stream 03/01 ✔ ✔ 10/01 ✘ ✔ 17/01 ✘ ✔ 24/01 ✔ ✔ 31/01 ✘ ✔ 07/02 ✘ ✔ 14/02 text/html 2019-08-06T20:40:00+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=aria_iii_contamination_2018&rev=1565116800&do=diff BD FACSAria III Pseudomonas contamination 2018 Date Sheath tank Stream 11/01 ✘ ✘ 22/01 ✘ ✘ 29/01 ✘ ✘ 31/01 ✘ ✔ 02/02 ✘ ✘ 14/02 ✘ ✔ 21/02 text/html 2026-04-13T17:28:45+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=aria_iiu_contamination&rev=1776094125&do=diff BD FACSAria IIu Pseudomonas contamination 2025 Date Sheath tank Stream 08/01 ✘ ✔ 17/01 ✘ ✔ 23/01 ✘ ✔ 30/01 ✘ ✔ 06/02 ✘ ✔ 13/02 ✘ ✔ 20/02 text/html 2019-08-06T11:49:27+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=aria_iiu_contamination_2018&rev=1565084967&do=diff BD FACSAria IIu Pseudomonas contamination 2018 Date Sheath tank Stream 29/01 ✘ ✘ 31/01 ✘ ✔ 02/02 ✘ ✘ 05/02 ✘ ✘ 14/02 ✔ ✔ 21/02 ✔ ✔ 27/02 text/html 2025-12-23T17:21:59+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=bd_accuri_c6&rev=1766506919&do=diff System overview The BD Accuri C6 Plus cell analyzer is an easy-to-use benchtop flow cytometer that allows for absolute cell counting and has a flexible optical layout. It has two lasers - 488 nm and 640 nm and four fluorescence detectors. The BD Accuri C6 Plus can acquire up to 10,000 events/second, with the data being digitally collected over a 7-decade dynamic range. Its low pressure pumping system allows for acquisition with any type of device (e.g. FACS, Eppendorf or Falcon tubes). Since th… text/html 2024-02-29T17:07:15+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=bd_accuri_c6_usage&rev=1709222835&do=diff BD Accuri C6 Usage Statistics bd_accuri_c6 Click here to get info on the BD Accuri C6 ---------- Back to Statistics text/html 2025-12-23T17:22:50+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=bd_facs_symphony_a5_se&rev=1766506970&do=diff Next Scheduled Maintenances:14 April System overview The BD FACSymphony™ A5 SE allows for high-parameter spectral flow cytometry. It enables you to gain full visible spectrum coverage to collect all light emitted without the need for filter changes, increasing dye flexibility and number of simultaneous colors. It also allows for autofluorescence unmixing, that may enable the resolution of difficult cells and populations. text/html 2024-03-01T15:56:22+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=bd_facs_symphony_a5_se_usage&rev=1709304982&do=diff BD FACSymphony A5 SE Usage Statistics bd_facs_symphony_a5_se Click here to get info on the BD FACSymphony A5 SE ---------- Back to Statistics text/html 2025-12-23T17:21:21+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=bd_facsaria_fusion&rev=1766506881&do=diff Next Scheduled Maintenances: 11 February and 20 August Pseudomonas contamination timeline System overview The BD FACSAria Fusion cell sorter is a high speed benchtop digital flow cytometer equipped with a sensitive, fixed-alignment cuvette flow cell. It has four spatially separated lasers - 405 nm, 488 nm, 561 nm and 640 nm. Its primary function is to analyze complex populations of cells and yield pure populations of cells meeting defined criteria. The sorter can purify samples that are sim… text/html 2024-02-29T17:06:46+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=bd_facsaria_fusion_usage&rev=1709222806&do=diff BD FACSAria Fusion Usage Statistics bd_facsaria_fusion Click here to get info on the BD FACSAria Fusion ---------- Back to Statistics text/html 2025-12-23T17:20:53+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=bd_facsaria_ii_oeiras&rev=1766506853&do=diff Next Scheduled Maintenances: 14 May and 28 November Pseudomonas contamination timeline System overview The BD FACSAria IIu cell sorter is a high speed benchtop digital flow cytometer equipped with a sensitive, fixed-alignment cuvette flow cell. It has 3 spatially separated lasers - 405 nm, 488 nm and 640 nm. Its primary function is to analyze complex populations of cells and yield pure populations of cells meeting defined criteria. The sorter can purify samples that are simply positive and … text/html 2025-12-23T17:21:12+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=bd_facsaria_iii&rev=1766506872&do=diff Next Scheduled Maintenances: 31 March and 15 September Pseudomonas contamination timeline System overview The BD FACSAria III cell sorter is a high speed benchtop digital flow cytometer equipped with a sensitive, fixed-alignment cuvette flow cell. It has four spatially separated lasers - 405 nm, 488 nm, 561 nm and 633 nm. Its primary function is to analyze complex populations of cells and yield pure populations of cells meeting defined criteria. The sorter can purify samples that are simply… text/html 2017-06-08T02:23:45+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=bd_facsaria_iii_quality&rev=1496881425&do=diff BD FACSAria III Quality Control - 2017 Click here to get info on the BD FACSAria III ---------- Back to Quality Control text/html 2024-02-29T16:53:51+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=bd_facsaria_iii_usage&rev=1709222031&do=diff BD FACSAria III Usage Statistics bd_facsaria_iii Click here to get info on the BD FACSAria III ---------- Back to Statistics text/html 2023-02-15T12:31:28+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=bd_facsaria_iiu&rev=1676460688&do=diff Next Scheduled Maintenances: - Pseudomonas contamination timeline System overview The BD FACSAria IIu cell sorter is a high speed benchtop digital flow cytometer equipped with a sensitive, fixed-alignment cuvette flow cell. It has three spatially separated lasers - 405 nm, 488 nm and 633 nm. Its primary function is to analyze complex populations of cells and yield pure populations of cells meeting defined criteria. The sorter can purify samples that are simply positive and negative for a sin… text/html 2017-06-08T02:23:53+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=bd_facsaria_iiu_quality&rev=1496881433&do=diff BD FACSAria IIu Quality Control - 2017 Click here to get info on the BD FACSAria IIu ---------- Back to Quality Control text/html 2017-05-02T17:22:55+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=bd_facsaria_iiu_repair&rev=1493738575&do=diff BD FACSAria IIu Repair History * 28/04/17 - A new violet laser has been installed on the BD FACSAria IIu * 18/04/17 - The violet laser on BD FACSAria IIu is not working ---------- Click here to get info on the BD FACSAria IIu text/html 2024-02-29T16:54:44+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=bd_facsaria_iiu_usage&rev=1709222084&do=diff BD FACSAria IIu Usage Statistics bd_facsaria_iiu Click here to get info on the BD FACSAria IIu ---------- Back to Statistics text/html 2024-02-29T17:07:41+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=bd_facscalibur2_usage&rev=1709222861&do=diff BD FACSCalibur 2 Usage Statistics ---------- Back to Statistics text/html 2024-02-29T17:07:33+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=bd_facscalibur_usage&rev=1709222853&do=diff BD FACSCalibur Usage Statistics ---------- Back to Statistics text/html 2024-02-29T17:07:48+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=bd_facscanto_usage&rev=1709222868&do=diff BD FACSCanto Usage Statistics ---------- Back to Statistics text/html 2025-12-23T17:21:40+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=bd_facsdiscover_s8&rev=1766506900&do=diff Next Scheduled Maintenances: Pseudomonas contamination timeline System overview The BD FACS Discover S8 is the first cell sorter that integrates imaging and full-spectrum capabilities. This versatile sorter offers both traditional sorting using standard fluorescent parameters, typical of conventional full-spectrum sorters, and advanced imaging features, which can be visualized through histograms and bivariate plots. This significant innovation allows cells to be sorted not only based on the … text/html 2025-01-03T16:19:31+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=bd_facsymphony_s6_se&rev=1735917571&do=diff Next Scheduled Maintenances: Pseudomonas contamination timeline System overview The BD FACSymphony S6 SE cell sorter is a high speed benchtop digital flow cytometer equipped with a sensitive, fixed-alignment cuvette flow cell. It has five spatially separated lasers - 349 nm, 405 nm, 488 nm, 561 nm and 633 nm. Its primary function is to analyze complex populations of cells and yield pure populations of cells meeting defined criteria. The sorter can purify samples that are simply positive and… text/html 2023-11-07T12:55:06+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=bd_lsr_fortessa_1&rev=1699358106&do=diff Next Scheduled Maintenances: 28 March and 26 September System overview The BD LSRFortessa 1 cell analyzer is a benchtop digital flow cytometer equipped with a fixed-alignment flow cell that is gel-coupled to the collection optics. It has three spatially separated lasers - 405 nm, 488 nm and 640 nm. Its primary function is to characterize complex populations of cells. Individual cells pass single file through a stream, which is interrogated by the three lasers with the emitted light being dete… text/html 2025-12-23T17:22:07+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=bd_lsr_fortessa_2&rev=1766506927&do=diff Next Scheduled Maintenances: 26 June and 11 December System overview The BD LSRFortessa cell analyzer is a benchtop digital flow cytometer equipped with a fixed-alignment flow cell that is gel-coupled to the collection optics. It has four spatially separated lasers - 405 nm, 488 nm, 561 nm and 640 nm. Its primary function is to characterize complex populations of cells. Individual cells pass single file through a stream, which is interrogated by the three lasers with the emitted light being d… text/html 2025-12-23T17:22:15+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=bd_lsr_fortessa_sorp&rev=1766506935&do=diff Next Scheduled Maintenances: 14 may and 28 November System overview The BD LSRFortessa cell analyzer is a benchtop digital flow cytometer equipped with a fixed-alignment flow cell that is gel-coupled to the collection optics. It has three spatially separated lasers - 442 nm, 488 nm and 561 nm. Its primary function is to characterize complex populations of cells. Individual cells pass single file through a stream, which is interrogated by the three lasers with the emitted light being detected … text/html 2025-12-23T17:22:27+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=bd_lsr_fortessa_x20&rev=1766506947&do=diff Next Scheduled Maintenances: 26 February and 20 August System overview The BD LSRFortessa X-20 cell analyzer is a benchtop digital flow cytometer equipped with a fixed-alignment flow cell that is gel-coupled to the collection optics. It has four spatially separated lasers - 405 nm, 488 nm, 561 nm and 640 nm. Its primary function is to characterize complex populations of cells. Individual cells pass single file through a stream, which is interrogated by the four lasers with the emitted light … text/html 2025-12-23T17:22:42+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=bd_lsr_fortessa_x20_oeiras&rev=1766506962&do=diff Next Scheduled Maintenances: 18 March and 5 November System overview The BD LSRFortessa X-20 cell analyzer is a benchtop digital flow cytometer equipped with a fixed-alignment flow cell that is gel-coupled to the collection optics. It has four spatially separated lasers - 405 nm, 488 nm, 561 nm and 640 nm. Its primary function is to characterize complex populations of cells. Individual cells pass single file through a stream, which is interrogated by the four lasers with the emitted light bei… text/html 2017-06-08T02:24:02+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=bd_lsrfortessa_1_quality&rev=1496881442&do=diff BD LSRFortessa 1 Quality Control - 2017 Click here to get info on the BD LSRFortessa 1 ---------- Back to Quality Control text/html 2024-02-29T17:08:04+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=bd_lsrfortessa_1_usage&rev=1709222884&do=diff BD LSRFortessa 1 Usage Statistics bd_lsr_fortessa_1 Click here to get info on the BD LSRFortessa 1 ---------- Back to Statistics text/html 2017-06-08T02:24:10+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=bd_lsrfortessa_2_quality&rev=1496881450&do=diff BD LSRFortessa 2 Quality Control - 2017 Click here to get info on the BD LSRFortessa 2 ---------- Back to Quality Control text/html 2024-02-29T17:06:55+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=bd_lsrfortessa_2_usage&rev=1709222815&do=diff BD LSRFortessa Usage Statistics bd_lsr_fortessa_2 Click here to get info on the BD LSRFortessa ---------- Back to Statistics text/html 2017-06-08T02:24:18+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=bd_lsrfortessa_x20_quality&rev=1496881458&do=diff BD LSRFortessa X-20 Quality Control - 2017 Click here to get info on the BD LSRFortessa X-20 ---------- Back to Quality Control text/html 2017-05-03T20:43:16+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=bd_lsrfortessa_x20_repair&rev=1493836996&do=diff BD LSRFortessa X-20 Repair History * 03/05/17 - The BD LSRFortessa X-20 is working again - the pressure transducer has been replaced * 02/05/17 - The BD LSRFortessa X-20 is not working ---- Click here to get info on the BD LSRFortessa X-20 text/html 2024-02-29T17:07:04+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=bd_lsrfortessa_x20_usage&rev=1709222824&do=diff BD LSRFortessa X-20 Usage Statistics bd_lsr_fortessa_x20 Click here to get info on the BD LSRFortessa X-20 ---------- Back to Statistics text/html 2026-04-12T23:01:37+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=billing&rev=1776027697&do=diff Our Usage Rules * Users must have a valid reservation in a system before using it. * Users must use their own booked time and not book or use a resource with other username. * The cell analyzers and cell sorters can be booked up to 1 month in advance text/html 2020-05-19T20:24:16+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=booking_warning&rev=1589912656&do=diff * Booking a slot until May 31st? → Please use the old booking system * Booking a slot from June 1st onwards? → Please use the new Agendo booking system ---------- Back to the Flow Cytometry Wiki text/html 2025-12-23T17:23:07+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=cytek_aurora&rev=1766506987&do=diff Next Scheduled Maintenances: 5 February & 24 July System overview The Cytek Aurora leverages full spectrum technology to provide flexibility to scientists, enabling the use of a wide array of new fluorochrome combinations without reconfiguring the system for each application. The Aurora system delivers high-resolution data at the single-cell level to resolve the most challenging cell populations, such as cells with high autofluorescence or low levels of expression of key biomarkers, regardle… text/html 2025-12-23T17:21:29+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=cytek_aurora_cs&rev=1766506889&do=diff Next Scheduled Maintenances: 9 April & 6 October Pseudomonas contamination timeline System overview The Cytek Aurora CS full spectrum technology provides flexibility, enabling the use of a wide array of new fluorochrome combinations without reconfiguring the system for each application. It delivers high-resolution data at the single-cell level to resolve the most challenging cell populations, such as cells with high autofluorescence or low levels of expression of key biomarkers, regardless … text/html 2026-04-13T17:29:13+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=cytek_aurora_cs_contamination&rev=1776094153&do=diff CYTEK Aurora CS Pseudomonas contamination 2025 Date Sheath tank Stream 08/01 ✘ ✘ 17/01 ✔ ✔ 23/01 ✔ ✔ 30/01 ✔ ✔ 06/02 ✔ ✔ 13/02 ✔ ✔ 20/02 text/html 2025-12-23T17:22:59+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=cytek_aurora_oeiras&rev=1766506979&do=diff Next Scheduled Maintenances: 5 February, 16 June and 18 December System overview The Cytek Aurora leverages full spectrum technology to provide flexibility to scientists, enabling the use of a wide array of new fluorochrome combinations without reconfiguring the system for each application. The Aurora system delivers high-resolution data at the single-cell level to resolve the most challenging cell populations, such as cells with high autofluorescence or low levels of expression of key biomar… text/html 2024-03-01T15:57:26+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=cytek_aurora_usage&rev=1709305046&do=diff Cytek Aurora Usage Statistics cytek_aurora Click here to get info on the Cytek Aurora ---------- Back to Statistics text/html 2026-04-10T15:06:29+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=dataanalysis&rev=1775826389&do=diff In addition to , we offer consultation sessions for users seeking to optimize their analysis workflows, explore new tools, and improve efficiency in data analysis. Our facility also provides access to and support for other software platforms, such as text/html 2024-01-22T13:07:14+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=filters&rev=1705925234&do=diff Which fluorochromes are compatible with the cytometers? Note: This is not a full list of all fluorochromes that are compatible with the flow cytometers. If you cannot find your fluorochrome of choice in the tables and are unsure whether a particular cytometer would work for you, please contact us. text/html 2025-04-03T13:04:14+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=flowcytometrytools&rev=1743678254&do=diff Software ---------- Back to the Flow Cytometry Wiki text/html 2025-12-23T17:23:18+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=imagestreamx&rev=1766506998&do=diff System overview The Cytek Amnis ImageStreamX is a benchtop multispectral imaging flow cytometer equipped with two CCD cameras that can acquire up to 12 images simultaneously of each cell or object in brightfield, scatter and multiple fluorescent channels at rates up to 5000 objects/sec. It has five lasers - 375 nm, 488 nm, 561 nm, 642 nm and 785 nm (dedicated to side scatter). Cells in suspension are hydrodynamically focused in a flow cell, where they are illuminated by a brightfield light sou… text/html 2018-03-06T18:16:38+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=mac_1&rev=1520356598&do=diff ---------- Back to the Resources page text/html 2017-07-13T14:36:58+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=mac_2&rev=1499949418&do=diff ---------- Back to the Resources page text/html 2025-04-03T13:07:26+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=macflow&rev=1743678446&do=diff Workstation overview The workstation is equipped with FlowJo Software and FCS Express. It is available free of charge, but please remember to book your session in advance through the Agendo system. If you need to analyse your data in FlowJo in text/html 2017-01-09T20:09:33+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=map&rev=1483988973&do=diff Where are the flow cytometers? ---------- Back to the Resources page text/html 2025-04-03T13:12:07+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=moonwalk&rev=1743678727&do=diff Workstation overview The Moonwalk is a high-performance data analysis workstation dedicated to analysis of spectral flow cytometry data with SpectroFlo. The workstation is also equipped with FlowJo Software with a shared license from the Flow Platform, and the text/html 2019-01-17T20:25:23+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=news_archive&rev=1547753123&do=diff * 17/01/19 - And the winners are... check the TOP 5 Users for 2018! * 10/01/19 - Check the Users Evaluation Survey 2018 results 2018 * 07/07/18 - And the winners are... check the TOP 5 Users for 2017! * 08/06/18 - The ImageStreamX is now available on the wiki * 04/04/18 - Check the new Charge Rates for 2018 * 10/01/18 text/html 2026-04-12T14:08:29+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=newuser&rev=1775995709&do=diff Access to our resources To use our equipment, you must register on . Follow the instructions: . You can ask permission to an equipment either by contacting us, or by clicking on the equipments’ calendar in Agendo. To access all systems (Cell Analysers, Cell Sorters and the Imaging Flow Cytometer) text/html 2026-04-10T19:00:54+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=paneldesign&rev=1775840454&do=diff Our Panel Design Service includes one-on-one consultations, tailored panel recommendations, and guidance on fluorophore selection based on antigen density, co-expression profiles, reagents available, and instrument compatibility. By providing this support to researchers, we aim to significantly reduce troubleshooting, optimize experimental outcomes, and improve the overall quality of the data generated. text/html 2026-04-12T13:18:35+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=past_events&rev=1775992715&do=diff Courses 2026 * 26 February: FLxFlow Tech's Café - Stable Fluorochromes that imporve your daily cytometry routine (Starring:BioRad´s StarBrights * 24 March : Cytolution Users Meeting * 14 April: OMIQ Webinar * 20 May: FLxFlow Tech's Café text/html 2025-04-03T12:56:50+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=pc&rev=1743677810&do=diff Workstation overview The workstation is equipped with FACSDiva Software and ModFit. You can use the workstation at no charge, but please remember to always book your session in the Agendo system. Useful Links ---------- Back to the Equipment page text/html 2024-01-17T18:44:40+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=prepare_samples&rev=1705513480&do=diff How to prepare samples for Flow Cytometry How do you get started? Talk to us about your experiment! If you want to use Flow Cytometry, we can help you design your new experiment. Get in contact with us and we will guide you through the Flow Cytometry Facility, and suggest the best systems for your project. Finally, we will book a training session with you and plan a trial run with your samples. text/html 2022-04-19T17:13:48+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=quality&rev=1650381228&do=diff Maintenance and Quality Control We perform daily Cytometer Setup & Tracking (CST) on our cytometers using BD CS&T research beads to track performance metrics such as detection efficiency, standard deviation of electronic noise, laser current, and optical background. text/html 2026-04-08T19:03:32+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=resources&rev=1775667812&do=diff Our equipment The Flow Cytometry Platform at GIMM is equipped with several instruments. The choice of the proper equipment should depend on: * the aims/applications of your project; * the combination of fluorochromes in your samples; * the text/html 2026-04-13T11:46:49+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=s8_contamination&rev=1776073609&do=diff BD FACSDiscover S8 Pseudomonas contamination 2025 Date Sheath tank Stream 10/01 ✘ ✔ 17/01 ✔ ✔ 24/01 ✘ ✘ 31/01 ✔ ✔ 14/02 ✔ ✔ 21/02 ✘ ✔ text/html 2026-04-12T14:13:35+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=start&rev=1775996015&do=diff ---------- ---------- ---------- ---------- text/html 2026-04-13T20:11:41+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=supportservices&rev=1776103901&do=diff Our Support Services Our Flow Cytometry Platform offers comprehensive support across the entire workflow, ensuring high-quality, reproducible results from project conception to data communication. We assist you at the initial stages, from grant writing text/html 2024-11-21T10:49:11+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=symphony_contamination&rev=1732182551&do=diff BD FACSymphony S6 SE Pseudomonas contamination 2023 Date Sheath tank Stream 15/12 ✘ ✔ 22/12 ✘ ✔ 29/12 ✘ ✔ BD FACSymphony S6 SE Pseudomonas contamination 2024 Date Sheath tank Stream text/html 2024-02-29T17:26:57+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=systems_usage_global_years&rev=1709224017&do=diff Global Usage Distribution ---------- Back to Statistics text/html 2018-05-28T20:11:13+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=systems_usage_years&rev=1527531073&do=diff Monthly Usage Hours Cell Sorters Cell Analyzers Imaging Flow Cytometer No longer available... ---------- Back to Statistics text/html 2026-01-08T21:43:55+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=tango&rev=1767905035&do=diff Workstation overview Tango is a remote high-performance data analysis workstation. If you have IGC credentials, you have access to FlowJo Software and also to IDEAS software and Machine Learning to analyse data from the Amnis ImageStream Please book on the Agendo calendar text/html 2026-04-12T12:40:48+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=team&rev=1775990448&do=diff Meet Our Team! ---------- Back to the Flow Cytometry Wiki text/html 2021-01-15T21:14:29+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=top5&rev=1610741669&do=diff Flow Cytometry Resources - TOP 5 Users And the winners are... 2020 Sónia Pereira (MMOTA) 400h Ana Cachucho (JBARATA) 327h Carolina Cunha (BSSANTOS) 290.5h 4 Joana Gonçalves (MMOTA) 284h 5 Sofia Mensurado (BSSANTOS) text/html 2026-04-09T23:42:16+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=trainingconventflow&rev=1775770936&do=diff To operate our equipments you must attend the 3 hour session on the Principles of Flow Cytometry. If you haven’t already, please book your Session. We will schedule the practical training session on the cell analyser, depending on the equipment availability and the planned date of your first experiment. Ideally, text/html 2026-04-10T15:04:05+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=trainingflowdata&rev=1775826245&do=diff At our platform, we provide training for users who are getting started with FlowJo. Our 2-hour FlowJo training session covers the basic features and a standard workflow using your own data. Assisted sessions are also available if needed. Our facility also provides access to and support for other software platforms, such as text/html 2026-04-10T15:08:36+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=trainingimagingflow&rev=1775826516&do=diff To operate our equipments you must attend the 3 hour session on the Principles of Flow Cytometry. If you haven’t already, please book your Session. We will schedule the practical training session on the imaging cell analyser, depending on the equipment availability and the planned date of your first experiment. Ideally, text/html 2026-04-12T23:10:11+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=trainingprinciplesflow&rev=1776028211&do=diff This theoretical session aims to provide a comprehensive understanding of the fundamental concepts of Flow Cytometry, how a flow cytometer works and what are the important concepts in experimental planning. This Session will also give you a detailed presentation of the Platform, the equipments available, the platform guidelines and policies, and all the services we provide to our users. text/html 2026-04-12T13:03:03+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=trainingselfsorting&rev=1775991783&do=diff How to become a power user We provide training in cell sorting to researchers falling into one of the following categories: * Projects involving a lot of cell sorts (1-2 x per week) for at least a couple of months. * Users who often need to sort outside of service hours (e.g. unexpected patient samples, which have to be processed immediately). text/html 2026-04-12T23:09:36+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=trainingspectralflow&rev=1776028176&do=diff To use our equipments you must attend the 3 hour session on the Principles of Flow Cytometry. If you haven’t already, please book your Session. To use our Spectral Analysers and Cell Sorters, you must attend a second theoretical session on the Principles of Spectral Flow Cytometry. text/html 2026-04-08T18:43:42+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=tutorials&rev=1775666622&do=diff Online Tutorials Basics of Flow Cytometry Spectral Flow Cytometry Planning Tools Fortessa/Fortessa X20 BD FACSymphony A5 SE/CYTEK Aurora ---------- Back to the Flow Cytometry Wiki text/html 2026-04-10T15:12:45+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=ucfservicesorting&rev=1775826765&do=diff Our Flow Cytometry Platform offers a Cell Purification Service by High Speed Cell Sorting. The staff will operate the cell sorter, and you just need to book the system on Agendo. Before your first experiment, we will contact you to know what kind of experiment you are going to perform and its details. This way, we can suggest the best equipment, the adequate nozzle size, collection conditions, sample preparation and any special requirements that may be needed. text/html 2025-07-09T00:14:19+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=usage_policy&rev=1752012859&do=diff Our Usage Rules Please download the detailed for your own reference. Cell Analyzers * Users must get trained on a cell analyzer before using it. Follow the New User guidelines to start using our equipment. * The cell analyzers can be booked up to 1 month in advance text/html 2024-08-23T11:04:46+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=usage_reports&rev=1724403886&do=diff Our Statistics users_feedback (click on graph for data since 2008) systems_usage_global_years Cell Sorters * BD FACSAria III * BD FACSAria Fusion Cell Analyzers * BD Accuri C6/C6 Plus * BD LSRFortessa * BD LSRFortessa X-20 * BD FACSymphony A5 SE * CYTEK Aurora Imaging Flow Cytometer * Amnis ImageStreamX No longer available * BD FACSCalibur * BD FACSCalibur 2 * BD FACSCanto * BD FACSAria IIu * BD LSRFortessa 1 ---------- Back to the Flow Cyto… text/html 2024-08-23T15:51:40+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=usefullinks&rev=1724421100&do=diff Planning Tools Fortessa/Fortessa X20 BD FACSymphony A5 SE/CYTEK Aurora ---------- Back to the Flow Cytometry Wiki text/html 2024-03-01T15:36:18+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=users_feedback&rev=1709303778&do=diff Users Evaluation Survey The iMM Satisfaction Survey aims at evaluating the services provided to researchers. Here are the results for the Flow Cytometry Unit. Thank you for your feedback! 2021 2022 2023 text/html 2026-04-12T23:45:19+02:00 https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=workstations&rev=1776030319&do=diff Flow cytometry data analysis can be performed either on-site at the Foxtrot workstation (available to all users) or remotely (restricted to Lisbon GIMM users). In all cases, analysis is conducted within a Mambo Area, where the required software is available (see table below).