Flow Cytometry Wiki

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===== Sample Preparation =====

  * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.005_-_how_to_prepare_samples_for_flow_cytometry.pdf|How to prepare samples for Flow Cytometry]]

===== Sample Preparation for Multicolor panel design =====

  * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.006_-_panel_design.pdf|Tips for Panel Design]] 

  * [[http://static.bdbiosciences.com/documents/Flow-Cytometry-Reagents-PS-Panels-with-Fortessa-X20.pdf?_ga=1.148042303.945356473.1478101143|Multicolor Panels Designed for Optimal Resolution ]]
  * [[http://static.bdbiosciences.com/documents/X20-Protocol-15-Color-Immunophenotyping.pdf?_ga=1.82680222.945356473.1478101143|15-Color Immunophenotyping]]
  * [[http://static.bdbiosciences.com/documents/X20-Protocol-System-5-Color-Panels.pdf?_ga=1.82680222.945356473.1478101143|5-Color Panels Designed for Minimal Compensation]]
  * [[http://static.bdbiosciences.com/documents/X20-Protocol-4-Color-Panels-Minimal-Spectral-Overlap.pdf?_ga=1.111997196.945356473.1478101143|4-Color Panels Minimal Spectral Overlap]]
  * [[http://static.bdbiosciences.com/documents/X20-Protocol-8-Color-Panels-for-Mesenchymal-Stromal-Cells.pdf?_ga=1.111997196.945356473.1478101143|8-Color Panels for Mesenchymal Stromal Cells]]

<html><a href="http://www.bdbiosciences.com/en-us/applications/research-applications/multicolor-flow-cytometry/product-selection-tools/spectrum-viewer"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_spectraviewer.jpg"></a></html>

<html><a href="http://www.lifetechnologies.com/europe-other/en/home/life-science/cell-analysis/labeling-chemistry/fluorescence-spectraviewer.html"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=fisher_spectraviewer.jpg"></a></html>

<html><a href="https://spectrum.cytekbio.com/"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=cytek_spectrum_viewer.png" width=500></a></html>

<html><a href="https://www.biolegend.com/spectraanalyzer"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=biolegend_spectraviewer.jpg" width=500></a></html>

<html><a href="http://www.bu.edu/flow-cytometry/files/2014/09/Fluorochrome-Chart-Relative-Brightness.pdf"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_fluorophore_brightness.jpg" width=500></a></html>

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