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usage_policy [2019/07/31 02:45]
flowcytometry
usage_policy [2024/02/28 22:35] (current)
flowcytometry [Cell Sorters]
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-<​html><​img src="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=usage_rules.png"><​BR><​BR></​html>​+<​html><​img src="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=usage_rules_v2_1.png"><​BR><​BR></​html>​
  
-Please download the detailed ​[[https://​imm.medicina.ulisboa.pt/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=sop.ucf.004_-_ucf_imm_user_guidelines.pdf|User Guidelines]] for your own reference.+Please download the detailed ​<​html><​a href="https://​imm.medicina.ulisboa.pt/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=sop.ucf.004_-_ucf_imm_user_guidelines.pdf" target="​_blank"> ​User Guidelines ​</​a></​html> ​for your own reference.
  
 ===== Cell Analyzers ===== ===== Cell Analyzers =====
-  - Users must get trained on a cell analyzer before using it. Use the booking system or [[imm-flowcytometry@medicina.ulisboa.pt|email us]] to request training. +  - Users must get trained on a cell analyzer before using it. Use the <​html><​a href="​https://​imm.agendoscience.com"​ target="​_blank"> ​booking system ​</​a></​html> ​or [[imm-flowcytometry@medicina.ulisboa.pt|email us]] to request training. 
-  - The cell analyzers **can be booked up to 2 months in advance**  +  - The cell analyzers **can be booked up to 2 months in advance**. 
-  - Users must **include 15 min in their booking time for cleaning** ​+  - Users must **include 15 min in their booking time for cleaning**.
   - Users must have a valid reservation in a system before using it.   - Users must have a valid reservation in a system before using it.
   - Users must use their own booked time and not book or use a resource with other username.   - Users must use their own booked time and not book or use a resource with other username.
-  - Users **can only cancel bookings 3h before the slot starting time** +  - Users **can only cancel bookings 3h before the slot starting time**. 
-  - Users must record start and finish times on the system log sheet (cleaning time included) and problems (if any) +  - Users must record start and finish times on the system log sheet (cleaning time included) and problems (if any). 
-  - Users that swap filters must leave the system with the default configuration +  - Users that swap filters must leave the system with the default configuration. 
-  - Users must log out from the analyzer software at the end of their session +  - Users must log out from the analyzer software at the end of their session. 
-  - Users must **switch off the system** if they are the **last user booked** or if the analyzer **will not be used for at least 3h** +  - Users must **switch off the system** if they are the **last user booked** or if the analyzer **will not be used for at least 3h** (Fortessa 2 & Fortessa X-20). 
-  - Users must always export their data and delete it from the FACSDiva database regularly +  - Users must always export their data and delete it from the FACSDiva/​SpectroFlo ​database regularly. 
-  - Users must **always clean the machines** following the cleaning protocol and **record the cleaning steps** in FACSDiva +  - Users must **always clean the machines** ​after acquisition ​following the cleaning protocol and **record the cleaning steps** in FACSDiva/​SpectroFlo. 
-  - Users must **check ​if the waste reservoir is full** (empty it if above the limit line) and **refill the sheath tank** with PBS+Azide at the end of the booked session.+  - Users must perform an additional cleaning before acquisition ​if they are the **first user of the day**.  
 +  - Users must **refill the sheath tank** with PBS+Azide at the end of the booked session
 +  - If during acquisition,​ the **waste container gets full**, a high pitch sound will go off. Users should **disconnect the black sensor** (the noise will stop), put the cytometer in **Standby**,​ and **disconnect 
 +the orange connector.** The container must be replaced with a new one.
  
 {{warning.jpg?​20|}} Users will be **billed according to the actual starting time or booked starting time (whichever occurs first) and the actual ending time or booked ending time (whichever occurs last)** {{warning.jpg?​20|}} Users will be **billed according to the actual starting time or booked starting time (whichever occurs first) and the actual ending time or booked ending time (whichever occurs last)**
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 ===== Cell Sorters ===== ===== Cell Sorters =====
   - Users must [[imm-flowcytometry@medicina.ulisboa.pt|contact us]] or use the booking system to request access to the cell sorters booking.   - Users must [[imm-flowcytometry@medicina.ulisboa.pt|contact us]] or use the booking system to request access to the cell sorters booking.
-  - The cell sorters **can be booked up to 2 months in advance** between 10am and 6pm +  - The cell sorters **can be booked up to 2 months in advance** between 10am and 6pm
   - If you need to book a sorter outside the regular working hours, please contact us beforehand to check for staff availability. ​   - If you need to book a sorter outside the regular working hours, please contact us beforehand to check for staff availability. ​
-  - Users must **include time in their booking for new settings, purity checks and cleaning** (minimum 30 min) +  - Users must **include time in their booking for new settings, purity checks and cleaning** (minimum 30 min). 
-  - Users must mention in the booking comments which nozzle (70 μm, 85 μm or 100 μm) and collection ​tubes (1.5 mL tubes, 5 mL FACS tubes or 15 mL Falcon tubes) they will be using +  - Users must mention in the booking comments ​the Biosafety Level (BSL) of their samples, ​which nozzle (70 μm, 85 μm or 100 μm) and which collection ​devices ​(plates, ​1.5 mL tubes, 5 mL FACS tubes or 15 mL Falcon tubes) they will be using. 
-  - Users must not book on behalf of others +  - Users must not book on behalf of others. 
-  - Users **can only cancel bookings 7 days in advance**+  - Users **can only cancel bookings 7 days in advance**.
  
 {{warning.jpg?​20|}} ** Cancellations** (within 6 days in advance) may result in a penalization \\  {{warning.jpg?​20|}} ** Cancellations** (within 6 days in advance) may result in a penalization \\ 
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 \\ \\
  
-{{warning.jpg?​20|}} ** NO SHOWS** ​(or cancellation in the booked day) - **the charge corresponds to the running cost of the system** +{{warning.jpg?​20|}} ** NO SHOWS** ​ - **the charge corresponds to the running cost of the system**
- +
-==== Sample Preparation ==== +
- +
-Please check **[[prepare_samples|How to prepare samples for Flow Cytometry]]**.+
  
 ===== Abuse Report ===== ===== Abuse Report =====
usage_policy.1564533921.txt.gz · Last modified: 2019/07/31 02:45 by flowcytometry