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usage_policy [2018/05/24 14:30]
flowcytometry
usage_policy [2021/11/18 22:25] (current)
flowcytometry
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-<​html><​img src="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=usage_rules.jpg"><​BR><​BR></​html>​+<​html><​img src="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=usage_rules_v2_1.png"><​BR><​BR></​html>​
  
 Please download the detailed [[https://​imm.medicina.ulisboa.pt/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=sop.ucf.004_-_ucf_imm_user_guidelines.pdf|User Guidelines]] for your own reference. Please download the detailed [[https://​imm.medicina.ulisboa.pt/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=sop.ucf.004_-_ucf_imm_user_guidelines.pdf|User Guidelines]] for your own reference.
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 ===== Cell Analyzers ===== ===== Cell Analyzers =====
   - Users must get trained on a cell analyzer before using it. Use the booking system or [[imm-flowcytometry@medicina.ulisboa.pt|email us]] to request training.   - Users must get trained on a cell analyzer before using it. Use the booking system or [[imm-flowcytometry@medicina.ulisboa.pt|email us]] to request training.
-  - The cell analyzers **can be booked up to 6 weeks in advance** ​+  - The cell analyzers **can be booked up to 2 months ​in advance** ​
   - Users must **include 15 min in their booking time for cleaning** ​   - Users must **include 15 min in their booking time for cleaning** ​
   - Users must have a valid reservation in a system before using it.   - Users must have a valid reservation in a system before using it.
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   - Users must **switch off the system** if they are the **last user booked** or if the analyzer **will not be used for at least 3h**   - Users must **switch off the system** if they are the **last user booked** or if the analyzer **will not be used for at least 3h**
   - Users must always export their data and delete it from the FACSDiva database regularly   - Users must always export their data and delete it from the FACSDiva database regularly
-  - Users must **always clean the machines** following the cleaning protocol and **record the cleaning steps** in FACSDiva +  - Users must **always clean the machines** ​after acquisition ​following the cleaning protocol and **record the cleaning steps** in FACSDiva 
-  - Users must **check ​if the waste reservoir is full** (empty it if above the limit line) and **refill the sheath tank** with PBS+Azide at the end of the booked session.+  - Users must perform an additional cleaning before acquisition ​if they are the **first user of the day**  
 +  - Users must **refill the sheath tank** with PBS+Azide at the end of the booked session
 +  - If during acquisition,​ the **waste container gets full**, a high pitch sound will go off. Users should **disconnect the black sensor** (the noise will stop), put the cytometer in **Standby**,​ and **disconnect 
 +the orange connector.** The container must be replaced with a new one.
  
 {{warning.jpg?​20|}} Users will be **billed according to the actual starting time or booked starting time (whichever occurs first) and the actual ending time or booked ending time (whichever occurs last)** {{warning.jpg?​20|}} Users will be **billed according to the actual starting time or booked starting time (whichever occurs first) and the actual ending time or booked ending time (whichever occurs last)**
  
-{{warning.jpg?​20|}} ** Last Minute Cancellations** (within 3h before the starting booked time) will result in a penalization \\ +{{warning.jpg?​20|}} ** Last Minute Cancellations** (within 3h before the starting booked time) may result in a penalization \\ 
 **The penalty charge corresponds to the running cost of the system if the session is not booked by another user** \\ **The penalty charge corresponds to the running cost of the system if the session is not booked by another user** \\
  
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 ===== Cell Sorters ===== ===== Cell Sorters =====
   - Users must [[imm-flowcytometry@medicina.ulisboa.pt|contact us]] or use the booking system to request access to the cell sorters booking.   - Users must [[imm-flowcytometry@medicina.ulisboa.pt|contact us]] or use the booking system to request access to the cell sorters booking.
-  - The cell sorters **can be booked up to 6 weeks in advance** between 10am and 6pm +  - The cell sorters **can be booked up to 2 months ​in advance** between 10am and 6pm  
 +  - If you need to book a sorter outside the regular working hours, please contact us beforehand to check for staff availability. ​
   - Users must **include time in their booking for new settings, purity checks and cleaning** (minimum 30 min)   - Users must **include time in their booking for new settings, purity checks and cleaning** (minimum 30 min)
-  - Users must mention in the booking comments which nozzle (70 μm, 85 μm or 100 μm) and collection ​tubes (1.5 mL tubes, 5 mL FACStubes ​or 15 mL Falcon tubes) they will be using+  - Users must mention in the booking comments ​the Biosafety Level (BSL) of their samples, ​which nozzle (70 μm, 85 μm or 100 μm) and which collection ​devices ​(plates, ​1.5 mL tubes, 5 mL FACS tubes or 15 mL Falcon tubes) they will be using
   - Users must not book on behalf of others   - Users must not book on behalf of others
   - Users **can only cancel bookings 7 days in advance**   - Users **can only cancel bookings 7 days in advance**
  
-{{warning.jpg?​20|}} ** Cancellations** (within 6 days in advance) ​will result in a penalization \\ +{{warning.jpg?​20|}} ** Cancellations** (within 6 days in advance) ​may result in a penalization \\ 
 **The penalty charge corresponds to the running cost of the system if the session is not booked by another user** ​ **The penalty charge corresponds to the running cost of the system if the session is not booked by another user** ​
 \\ \\
  
-{{warning.jpg?​20|}} ** NO SHOWS** ​(1 day before or on the same day) - **the charge corresponds to the running cost of the system** +{{warning.jpg?​20|}} ** NO SHOWS** ​ - **the charge corresponds to the running cost of the system**
- +
-==== Sample Preparation ==== +
- +
-Please check **[[prepare_samples|How to prepare samples for Flow Cytometry]]**.+
  
 ===== Abuse Report ===== ===== Abuse Report =====
usage_policy.1527165009.txt.gz · Last modified: 2018/05/24 14:30 by flowcytometry