Flow Cytometry Wiki

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Please download the detailed [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.004_-_ucf_imm_user_guidelines.pdf|User Guidelines]] for your own reference.

===== Cell Analyzers =====
  - Users must get trained on a cell analyzer before using it. Use the booking system or [[imm-flowcytometry@medicina.ulisboa.pt|email us]] to request training.
  - The cell analyzers **can be booked up to 6 weeks in advance** 
  - Users must **include 15 min in their booking time for cleaning** 
  - Users must have a valid reservation in a system before using it.
  - Users must use their own booked time and not book or use a resource with other username.
  - Users **can only cancel bookings 3h before the slot starting time**
  - Users must record start and finish times on the system log sheet (cleaning time included) and problems (if any)
  - Users that swap filters must leave the system with the default configuration
  - Users must log out from the analyzer software at the end of their session
  - Users must **switch off the system** if they are the **last user booked** or the analyzer **will not be used for at least 3h**
  - Users must always export their data and delete it from the FACSDiva database regularly
  - Users must **always clean the machines** following the cleaning protocol and **record the cleaning steps** in FACSDiva
  - Users must **check if the waste reservoir is full** (empty it if above the limit line) and **refill the sheath tank** with PBS+Azide at the end of the booked session.

{{warning.jpg?20|}} Users will be **billed according to the actual starting time or booked starting time (whichever occurs first) and the actual ending time or booked ending time (whichever occurs last)**

{{warning.jpg?20|}} ** Last Minute Cancellations** (within 3h before the starting booked time) will result in a penalization \\ 
**The penalty charge corresponds to the running cost of the system if the session is not booked by another user** \\

{{warning.jpg?20|}} ** NO SHOWS** - **the charge corresponds to the running cost of the system**

===== Cell Sorters =====
  - Users must [[imm-flowcytometry@medicina.ulisboa.pt|contact us]] or use the booking system to request access to the cell sorters booking.
  - The cell sorters **can be booked up to 6 weeks in advance** between 10am and 6pm 
  - Users must **include time in their booking for new settings, purity checks and cleaning** (minimum 30 min)
  - Users must mention in the booking comments which nozzle (70 μm, 85 μm or 100 μm) and collection tubes (1.5 mL tubes, 5 mL FACStubes or 15 mL Falcon tubes) they will be using
  - Users must not book on behalf of others
  - Users **can only cancel bookings 7 days in advance**

{{warning.jpg?20|}} ** Cancellations** (within 6 days in advance) must be requested by [[imm-flowcytometry@medicina.ulisboa.pt|email]] \\ 
**The penalty charge corresponds to the running cost of the system if the session is not booked by another user** 
\\

{{warning.jpg?20|}} ** NO SHOWS** (1 day before or on the same day) - **the charge corresponds to the running cost of the system**

==== Sample Preparation ====

Please check **[[prepare_samples|How to prepare samples for Flow Cytometry]]**.

===== Abuse Report =====
If you find a user that did not comply with the above rules please [[imm-flowcytometry@medicina.ulisboa.pt|contact us]].

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