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usage_policy [2021/03/29 17:37] flowcytometry |
usage_policy [2024/02/28 22:34] flowcytometry |
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- | <html><img src="/facility/flowcytometry/lib/exe/fetch.php?media=usage_rules.png"><BR><BR></html> | + | <html><img src="/facility/flowcytometry/lib/exe/fetch.php?media=usage_rules_v2_1.png"><BR><BR></html> |
- | Please download the detailed [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.004_-_ucf_imm_user_guidelines.pdf|User Guidelines]] for your own reference. | + | Please download the detailed <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.004_-_ucf_imm_user_guidelines.pdf" target="_blank"> User Guidelines </a></html> for your own reference. |
===== Cell Analyzers ===== | ===== Cell Analyzers ===== | ||
- | - Users must get trained on a cell analyzer before using it. Use the booking system or [[imm-flowcytometry@medicina.ulisboa.pt|email us]] to request training. | + | - Users must get trained on a cell analyzer before using it. Use the <html><a href="https://imm.agendoscience.com" target="_blank"> booking system </a></html> or [[imm-flowcytometry@medicina.ulisboa.pt|email us]] to request training. |
- | - The cell analyzers **can be booked up to 2 months in advance** | + | - The cell analyzers **can be booked up to 2 months in advance**. |
- | - Users must **include 15 min in their booking time for cleaning** | + | - Users must **include 15 min in their booking time for cleaning**. |
- Users must have a valid reservation in a system before using it. | - Users must have a valid reservation in a system before using it. | ||
- Users must use their own booked time and not book or use a resource with other username. | - Users must use their own booked time and not book or use a resource with other username. | ||
- | - Users **can only cancel bookings 3h before the slot starting time** | + | - Users **can only cancel bookings 3h before the slot starting time**. |
- | - Users must record start and finish times on the system log sheet (cleaning time included) and problems (if any) | + | - Users must record start and finish times on the system log sheet (cleaning time included) and problems (if any). |
- | - Users that swap filters must leave the system with the default configuration | + | - Users that swap filters must leave the system with the default configuration. |
- | - Users must log out from the analyzer software at the end of their session | + | - Users must log out from the analyzer software at the end of their session. |
- | - Users must **switch off the system** if they are the **last user booked** or if the analyzer **will not be used for at least 3h** | + | - Users must **switch off the system** if they are the **last user booked** or if the analyzer **will not be used for at least 3h** (Fortessa 2 & Fortessa X-20). |
- | - Users must always export their data and delete it from the FACSDiva database regularly | + | - Users must always export their data and delete it from the FACSDiva/SpectroFlo database regularly. |
- | - Users must **always clean the machines** after acquisition following the cleaning protocol and **record the cleaning steps** in FACSDiva | + | - Users must **always clean the machines** after acquisition following the cleaning protocol and **record the cleaning steps** in FACSDiva/SpectroFlo. |
- | - Users must perform an additional cleaning before acquisition if they are the **first user of the day** | + | - Users must perform an additional cleaning before acquisition if they are the **first user of the day**. |
- Users must **refill the sheath tank** with PBS+Azide at the end of the booked session. | - Users must **refill the sheath tank** with PBS+Azide at the end of the booked session. | ||
- If during acquisition, the **waste container gets full**, a high pitch sound will go off. Users should **disconnect the black sensor** (the noise will stop), put the cytometer in **Standby**, and **disconnect | - If during acquisition, the **waste container gets full**, a high pitch sound will go off. Users should **disconnect the black sensor** (the noise will stop), put the cytometer in **Standby**, and **disconnect | ||
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- | {{warning.jpg?20|}} ** NO SHOWS** (or cancellation in the booked day) - **the charge corresponds to the running cost of the system** | + | {{warning.jpg?20|}} ** NO SHOWS** - **the charge corresponds to the running cost of the system** |
- | + | ||
- | ==== Sample Preparation ==== | + | |
- | + | ||
- | Please check **[[prepare_samples|How to prepare samples for Flow Cytometry]]**. | + | |
===== Abuse Report ===== | ===== Abuse Report ===== |