User Tools

Site Tools


usage_policy

Differences

This shows you the differences between two versions of the page.

Link to this comparison view

Both sides previous revision Previous revision
Next revision
Previous revision
Last revision Both sides next revision
usage_policy [2018/05/23 16:45]
flowcytometry [Cell Analyzers]
usage_policy [2021/11/18 14:37]
flowcytometry
Line 1: Line 1:
-<​html><​img src="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=usage_rules.jpg"><​BR><​BR></​html>​+<​html><​img src="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=usage_rules_2.png"><​BR><​BR></​html>​
  
 Please download the detailed [[https://​imm.medicina.ulisboa.pt/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=sop.ucf.004_-_ucf_imm_user_guidelines.pdf|User Guidelines]] for your own reference. Please download the detailed [[https://​imm.medicina.ulisboa.pt/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=sop.ucf.004_-_ucf_imm_user_guidelines.pdf|User Guidelines]] for your own reference.
Line 5: Line 5:
 ===== Cell Analyzers ===== ===== Cell Analyzers =====
   - Users must get trained on a cell analyzer before using it. Use the booking system or [[imm-flowcytometry@medicina.ulisboa.pt|email us]] to request training.   - Users must get trained on a cell analyzer before using it. Use the booking system or [[imm-flowcytometry@medicina.ulisboa.pt|email us]] to request training.
-  - The cell analyzers **can be booked up to 6 weeks in advance** ​+  - The cell analyzers **can be booked up to 2 months ​in advance** ​
   - Users must **include 15 min in their booking time for cleaning** ​   - Users must **include 15 min in their booking time for cleaning** ​
   - Users must have a valid reservation in a system before using it.   - Users must have a valid reservation in a system before using it.
Line 13: Line 13:
   - Users that swap filters must leave the system with the default configuration   - Users that swap filters must leave the system with the default configuration
   - Users must log out from the analyzer software at the end of their session   - Users must log out from the analyzer software at the end of their session
-  - Users must **switch off the system** if they are the **last user booked** or the analyzer **will not be used for at least 3h**+  - Users must **switch off the system** if they are the **last user booked** or if the analyzer **will not be used for at least 3h**
   - Users must always export their data and delete it from the FACSDiva database regularly   - Users must always export their data and delete it from the FACSDiva database regularly
-  - Users must **always clean the machines** following the cleaning protocol and **record the cleaning steps** in FACSDiva +  - Users must **always clean the machines** ​after acquisition ​following the cleaning protocol and **record the cleaning steps** in FACSDiva 
-  - Users must **check ​if the waste reservoir is full** (empty it if above the limit line) and **refill the sheath tank** with PBS+Azide at the end of the booked session.+  - Users must perform an additional cleaning before acquisition ​if they are the **first user of the day**  
 +  - Users must **refill the sheath tank** with PBS+Azide at the end of the booked session
 +  - If during acquisition,​ the **waste container gets full**, a high pitch sound will go off. Users should **disconnect the black sensor** (the noise will stop), put the cytometer in **Standby**,​ and **disconnect 
 +the orange connector.** The container must be replaced with a new one.
  
 {{warning.jpg?​20|}} Users will be **billed according to the actual starting time or booked starting time (whichever occurs first) and the actual ending time or booked ending time (whichever occurs last)** {{warning.jpg?​20|}} Users will be **billed according to the actual starting time or booked starting time (whichever occurs first) and the actual ending time or booked ending time (whichever occurs last)**
  
-{{warning.jpg?​20|}} ** Last Minute Cancellations** (within 3h before the starting booked time) will be penalized ​\\ +{{warning.jpg?​20|}} ** Last Minute Cancellations** (within 3h before the starting booked time) may result in a penalization ​\\ 
 **The penalty charge corresponds to the running cost of the system if the session is not booked by another user** \\ **The penalty charge corresponds to the running cost of the system if the session is not booked by another user** \\
  
Line 27: Line 30:
 ===== Cell Sorters ===== ===== Cell Sorters =====
   - Users must [[imm-flowcytometry@medicina.ulisboa.pt|contact us]] or use the booking system to request access to the cell sorters booking.   - Users must [[imm-flowcytometry@medicina.ulisboa.pt|contact us]] or use the booking system to request access to the cell sorters booking.
-  - The cell sorters **can be booked up to 6 weeks in advance** between 10am and 6pm +  - The cell sorters **can be booked up to 2 months ​in advance** between 10am and 6pm  
 +  - If you need to book a sorter outside the regular working hours, please contact us beforehand to check for staff availability. ​
   - Users must **include time in their booking for new settings, purity checks and cleaning** (minimum 30 min)   - Users must **include time in their booking for new settings, purity checks and cleaning** (minimum 30 min)
-  - Users must mention in the booking comments which nozzle (70 μm, 85 μm or 100 μm) and collection ​tubes (1.5 mL tubes, 5 mL FACStubes ​or 15 mL Falcon tubes) they will be using+  - Users must mention in the booking comments ​the Biosafety Level (BSL) of their samples, ​which nozzle (70 μm, 85 μm or 100 μm) and which collection ​devices ​(plates, ​1.5 mL tubes, 5 mL FACS tubes or 15 mL Falcon tubes) they will be using
   - Users must not book on behalf of others   - Users must not book on behalf of others
   - Users **can only cancel bookings 7 days in advance**   - Users **can only cancel bookings 7 days in advance**
  
-{{warning.jpg?​20|}} ** Cancellations** (within 6 days in advance) ​must be requested by [[imm-flowcytometry@medicina.ulisboa.pt|email]] ​\\ +{{warning.jpg?​20|}} ** Cancellations** (within 6 days in advance) ​may result in a penalization ​\\ 
 **The penalty charge corresponds to the running cost of the system if the session is not booked by another user** ​ **The penalty charge corresponds to the running cost of the system if the session is not booked by another user** ​
 \\ \\
  
-{{warning.jpg?​20|}} ** NO SHOWS** ​(1 day before or on the same day) - **the charge corresponds to the running cost of the system** +{{warning.jpg?​20|}} ** NO SHOWS** ​ - **the charge corresponds to the running cost of the system**
- +
-==== Sample Preparation ==== +
- +
-Please check **[[prepare_samples|How to prepare samples for Flow Cytometry]]**.+
  
 ===== Abuse Report ===== ===== Abuse Report =====
usage_policy.txt · Last modified: 2021/11/18 22:25 by flowcytometry