Flow Cytometry Wiki

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To operate our equipments you must attend the 2,5 hour session on the Principles of Flow Cytometry. If you haven’t already, please [[TrainingPrinciplesFlow|book your Session]]. 

To use our Spectral Analysers, you must attend a __second theoretical session on the Principles of Spectral Flow Cytometry.__ 

This theoretical training covers the fundamental concepts of Spectral Flow Cytometry, and the differences in relation to Conventional Flow Cytometry. You’ll also be learning important concepts in experimental planning, sample preparation guidelines and panel design, fundamental topics for a spectral workflow. 
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__Please add your name and group in one of the following dates:__

^  Principles of Spectral Flow Cytometry  ^  
|  <html><a href="https://docs.google.com/spreadsheets/d/1kdDHJSgv56pa8xhGi9O9WbYzw90Lb9riKDwtb7IVak0/edit?usp=sharing">06/02/2024</a></html>  |  
|  <html><a href="https://docs.google.com/spreadsheets/d/1kdDHJSgv56pa8xhGi9O9WbYzw90Lb9riKDwtb7IVak0/edit?usp=sharing">05/03/2024</a></html>  |  
|  <html><a href="https://docs.google.com/spreadsheets/d/1kdDHJSgv56pa8xhGi9O9WbYzw90Lb9riKDwtb7IVak0/edit?usp=sharing">02/04/2024</a></html>  |  
|  <html><a href="https://docs.google.com/spreadsheets/d/1kdDHJSgv56pa8xhGi9O9WbYzw90Lb9riKDwtb7IVak0/edit?usp=sharing">30/04/2024</a></html>  |  
|  <html><a href="https://docs.google.com/spreadsheets/d/1kdDHJSgv56pa8xhGi9O9WbYzw90Lb9riKDwtb7IVak0/edit?usp=sharing">04/06/2024</a></html>  |  

We will schedule the practical training session on the spectral cell analyser, depending on the equipment availability and the planned date of your first experiment. Ideally, __you should be trained a few days before your first usage__. 

After the training, you will be kept as an “in training” user on the Agendo booking system for the next few sessions and will be given full permission as soon as you are autonomously using the cytometers.
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====Be sure to read the following documents prior to your practical training:====

  * <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=SOP.UCF.037_-_BD_FACSDiva_BD_FACSymphony_A5_Tutorial.pdf" target="_blank">DIVA Tutorial</a></html> (BD FACSymphony A5 SE)
  * <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sopxx.pdf" target="_blank">SpectroFlo Tutorial</a></html> (Cytek Aurora)

  * <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.004_-_ucf_imm_user_guidelines.pdf" target="_blank">User Guidelines</a></html>
  * <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=prepare_samples" target="_blank">How to prepare samples for Flow Cytometry</a></html>

====Check our Spectral Analysers====
<html><td  style="border:0px solid white;">
<p style="text-align:left"><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=bd_facs_symphony_a5_se" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=facsymphony_wiki_page_name.png" width=175></a><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=cytek_aurora" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=aurora_wiki_page_name.png" width=150></a></p></td>
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