Flow Cytometry Wiki

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To operate our equipments you must attend the 2 hour session on the Principles of Flow Cytometry. If you haven’t already, please [[TrainingPrinciplesFlow|book your Session]]. 

To use our Spectral Analysers, you must attend a __second theoretical session on the Principles of Spectral Flow Cytometry.__ 

This theoretical training covers the fundamental concepts of Spectral Flow Cytometry, and the differences in relation to Conventional Flow Cytometry. You’ll also be learning important concepts in experimental planning, sample preparation guidelines and panel design, fundamental topics for a spectral workflow. 
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Please book your training:

^  Principles of Spectral Flow Cytometry  ^  
|  <del>10/01/2024</del>  | 
|  <html><a href="https://my.agendoscience.com/calendar/?id=NDFjYWxlbmRhcnM=" target="_blank">10/02/2024</a></html>  |  
|  22/02/2024  |  
|  14/03/2024  | 
|  04/04/2024  |  
|  24/04/2024  | 

We will schedule the practical training session on the spectral cell analyser, depending on the equipment availability and the planned date of your first experiment. Ideally, __you should be trained on the same day or a few days before your first usage__. 

**Make sure you read the Important Information documents below**.

After the training, you will be kept as an “in training” user on the Agendo booking system for the next few sessions and will be given full permission as soon as you are autonomously using the cytometers.
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====Important information:====

  * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=SOP.UCF.037_-_BD_FACSDiva_BD_FACSymphony_A5_Tutorial.pdf|DIVA Tutorial]] (BD FACSymphony A5 SE)
  * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sopxx.pdf|SpectroFlo Tutorial]] (Cytek Aurora)

  * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.004_-_ucf_imm_user_guidelines.pdf|User Guidelines]]
  * [[prepare_samples|How to prepare samples for Flow Cytometry]]

===Check our Conventional Analysers===
 
<html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=resources" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=equipment_v2.png" width=120></a></html>
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