sorting_faq
Differences
This shows you the differences between two versions of the page.
| Both sides previous revision Previous revision Next revision | Previous revision | ||
|
sorting_faq [2019/09/04 18:48] flowcytometry [How are my cells collected?] |
sorting_faq [2021/03/29 19:19] flowcytometry removed |
||
|---|---|---|---|
| Line 16: | Line 16: | ||
| We can **adjust the temperature** of the sorters to 4 ºC, 20 ºC, 37 ºC and 42 ºC, and we can also keep your cells on ice, if that is what you need. | We can **adjust the temperature** of the sorters to 4 ºC, 20 ºC, 37 ºC and 42 ºC, and we can also keep your cells on ice, if that is what you need. | ||
| - | The BD FACSAria sorters faster with a smaller nozzle at higher pressure. With the 70 µm nozzle, you can sort up to 20,000 events/second. This is a great choice for small cells that are happy in single-cell suspension, for example blood and bone marrow. Larger cells need a larger nozzle size. Your cells should be no more than one-third the size of the nozzle. Cells that are fragile or easily stressed (e.g. cultured cells) need a lower pressure sort (100 µm nozzle). Our sorters have the following options: | + | The BD FACSAria sorts faster with a smaller nozzle at higher pressure. With the 70 µm nozzle, you can sort up to 20,000 events/second. This is a great choice for small cells that are happy in single-cell suspension, for example blood and bone marrow. Larger cells need a larger nozzle size. Your cells should be no more than one-third the size of the nozzle. Cells that are fragile or easily stressed (e.g. cultured cells) need a lower pressure sort (100 µm nozzle). Our sorters have the following options: |
| * 70 µm nozzle / 70 psi | * 70 µm nozzle / 70 psi | ||
| * 85 µm nozzle / 45 psi | * 85 µm nozzle / 45 psi | ||
Page Tools
