Flow Cytometry Wiki

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The UCF at iMM is equipped with several instruments.
The choice of the proper equipment should depend on: 
  * the **aims/applications of your project**;
  * the **combination of fluorochromes** in your samples;
  * the **optical configuration** required to detect your fluorochrome panel;
  * the **availability of the equipment**.  

The following table summarizes the excitation optics of all our flow cytometers. To find out more about each instrument's optical configuration, see [[filters|the fluorochromes compatible with the cytometers]] and the individual descriptions bellow.

{{ exc_lasers_imm.jpg?700 }}

{{maps-icon.png?25|}} [[map|Where are the flow cytometers?]]

{{list-icon.png?23|}} [[quality|How are the flow cytometers?]] (Quality Control)

{{:info.gif?25|}} [[filters|Which fluorochromes are compatible with the cytometers?]]

==== Cell Sorters ====
  * [[bd_facsaria_iii|{{::aria_iii.png?150}}]] [[bd_facsaria_iii|BD FACSAria III]] -> <html><a href="https://my.agendo.science/calendar/?id=NDVjYWxlbmRhcnM="><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png"> Booking</a></html>
  * [[bd_facsaria_iiu|{{::aria_iiu.png?150|}}]] [[bd_facsaria_iiu|BD FACSAria IIu]] -> <html><a href="https://my.agendo.science/calendar/?id=NDRjYWxlbmRhcnM="><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png"> Booking</a></html>

==== Cell Analyzers ====
  * [[bd_lsr_fortessa_1|{{::fortessa_1.png?120|}}]] [[bd_lsr_fortessa_1|BD LSRFortessa 1]] -> <html><a href="https://my.agendo.science/calendar/?id=NDBjYWxlbmRhcnM="><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png"> Booking</a></html>
  * [[bd_lsr_fortessa_2|{{::fortessa_2.png?120|}}]] [[bd_lsr_fortessa_2|BD LSRFortessa 2]] -> <html><a href="https://my.agendo.science/calendar/?id=NDFjYWxlbmRhcnM="><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png"> Booking</a></html>
  * [[bd_lsr_fortessa_x20|{{::fortessa_x20.png?120|}}]] [[bd_lsr_fortessa_x20|BD LSRFortessa X-20]] -> <html><a href="https://my.agendo.science/calendar/?id=NDJjYWxlbmRhcnM="><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png"> Booking</a></html>
  * [[bd_accuri_c6|{{::accuri.png?120|}}]] [[bd_accuri_c6|BD Accuri C6 Plus]] -> <html><a href="https://my.agendo.science/calendar/?id=NDNjYWxlbmRhcnM="><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png"> Booking</a></html>

==== Imaging Flow Cytometer ====
  * [[ImageStreamX|{{::imagestream.png?120|}}]] [[ImageStreamX|Amnis ImageStreamX]] -> <html><a href="https://my.agendo.science/calendar/?id=MTBjYWxlbmRhcnM="><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png"> Booking</a></html>


==== Workstations ====
{{::macflow.png?100|}} 

  * **MacFlow 1** - With FlowJo Software -> <html><a href="https://my.agendo.science/calendar/?id=NDZjYWxlbmRhcnM="><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png"> Booking</a></html>

  * **MacFlow 2 (new)** - With FlowJo Software -> <html><a href="https://my.agendo.science/calendar/?id=NDZjYWxlbmRhcnM="><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png"> Booking</a></html>

{{:pc_workstation.jpg?100|}}     
  * **PC** - With FlowJo, FACSDiva and ModFitLT Software -> <html><a href="https://my.agendo.science/calendar/?id=MTUxY2FsZW5kYXJz"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png"> Booking</a></html>

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