quality
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quality [2017/04/13 16:52] flowcytometry |
quality [2022/04/19 17:13] (current) flowcytometry |
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| - | ====== Microscope Quality Control ====== | + | ====== Maintenance and Quality Control ====== |
| - | ==== CST ==== | + | {{ cs_t_beads.png?700 }} |
| - | {{CSTs.png |}} We perform daily Cytometer Setup & Tracking (CST) on our cytometers using BD CS&T research beads to track performance metrics such as detection efficiency, standard deviation of electronic noise, laser current, and optical background.\\ | + | We perform daily **Cytometer Setup & Tracking (CST)** on our cytometers using BD CS&T research beads to track performance metrics such as detection efficiency, standard deviation of electronic noise, laser current, and optical background.\\ |
| - | In the quality control plots below you can check one of the measured parameters, the **Bright Bead % Robust CV** for the primary channel of each laser present in the system, for the first day of each month.\\ | + | If there is a major alteration over time, we troubleshoot the problem to solve it as promptly as possible. |
| - | Please contact us if you want to check the CST values for a particular date. | + | |
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| + | {{ rainbow_beads.png?1000 }} | ||
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| + | We also run **Rainbow 8 peak Beads (RB)** as a routine calibration of the flow cytometers to evaluate the linearity of the system. | ||
| + | \\ | ||
| + | These RB 8 peak beads are also specific for following the EuroFlow(TM) standardization guidelines.\\ | ||
| + | |||
| + | Please contact us if you want to check the CST or RB values for a particular date. | ||
| + | \\ | ||
| + | \\ | ||
| + | ==== Pseudomonas Contamination Timeline ==== | ||
| - | ==== Check the Systems ==== | + | [[aria_fusion_contamination|BD FACSAria Fusion]] |
| - | === Cell Sorters === | + | [[aria_iii_contamination|BD FACSAria III]] |
| - | * [[bd_facsaria_iii_quality|BD FACSAria III]] | + | |
| - | * [[bd_facsaria_iiu_quality|BD FACSAria IIu]] | + | |
| - | === Cell Analyzers === | + | [[aria_iiu_contamination|BD FACSAria IIu]] (no longer available...) |
| - | * [[bd_lsrfortessa_1_quality|BD LSRFortessa 1]] | + | |
| - | * [[bd_lsrfortessa_1_quality|BD LSRFortessa 2]] | + | |
| - | * [[bd_lsrfortessa_x20_quality|BD LSRFortessa X-20]] | + | |
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| [[resources|Back to the Resources page]] | [[resources|Back to the Resources page]] | ||
quality.1492095157.txt.gz · Last modified: 2017/04/13 16:52 by flowcytometry
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