Flow Cytometry Wiki

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====== Flow Cytometers Quality Control ======

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We perform daily **Cytometer Setup & Tracking (CST)** on our cytometers using BD CS&T research beads to track performance metrics such as detection efficiency, standard deviation of electronic noise, laser current, and optical background.\\
If there is a major alteration over time, we troubleshoot the problem to solve it as promptly as possible. 
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{{  rainbow_beads.png?1000  }} 
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We also run **Rainbow 8 peak Beads (RB)** as a routine calibration of the flow cytometers to evaluate the linearity of the system. 
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These RB 8 peak beads are also specific for following the EuroFlow(TM) standardization guidelines.\\

Please contact us if you want to check the CST or RB values for a particular date.
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==== Pseudomonas Contamination Timeline ====

[[aria_fusion_contamination|BD FACSAria Fusion]]

[[aria_iii_contamination|BD FACSAria III]]

[[aria_iiu_contamination|BD FACSAria IIu]] (no longer available...)

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