prepare_samples
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prepare_samples [2024/01/17 18:33] flowcytometry |
prepare_samples [2024/01/17 18:44] (current) flowcytometry [How do you get started?] |
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| Talk to us about your experiment! If you want to use Flow Cytometry, we can help you design your new experiment. Get in contact with us and we will guide you through the Flow Cytometry Facility, and suggest the best systems for your project. Finally, we will book a training session with you and plan a trial run with your samples. | Talk to us about your experiment! If you want to use Flow Cytometry, we can help you design your new experiment. Get in contact with us and we will guide you through the Flow Cytometry Facility, and suggest the best systems for your project. Finally, we will book a training session with you and plan a trial run with your samples. | ||
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| + | Here you can find summed up information on the essentials of handling your samples. | ||
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| + | For more info check our **full document** on <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.005_-_how_to_prepare_samples_for_flow_cytometry.pdf" target="_blank"> How to prepare cell samples for Flow Cytometry</a></html>. | ||
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| **__Exclude your dead cells__** | **__Exclude your dead cells__** | ||
| - | Even though you keep your cells as happy as possible, some cells can die while handling and performing staining protocols. \\ Dead cells can affect the analysis by compromising the integrity of the data by non-specifically binding antibodies. That’s why most experiments benefit from adding a viability dye, to exclude dead cells from analysis and sorting. Also this viability staining can help you evaluate and compare the cell apoptosis and necrotic stages between cell conditions.\\ There are Fixable and Non-Fixable viability dyes. You can choose the right one for your assay depending on the purpose of your analysis and the post-staining protocol that will be performed. For more info check our full document on **[[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.005_-_how_to_prepare_samples_for_flow_cytometry.pdf|How to prepare cell samples]]**. | + | Even though you keep your cells as happy as possible, some cells can die while handling and performing staining protocols. \\ Dead cells can affect the analysis by compromising the integrity of the data by non-specifically binding antibodies. That’s why most experiments benefit from adding a viability dye, to exclude dead cells from analysis and sorting. Also this viability staining can help you evaluate and compare the cell apoptosis and necrotic stages between cell conditions.\\ There are Fixable and Non-Fixable viability dyes. You can choose the right one for your assay depending on the purpose of your analysis and the post-staining protocol that will be performed. . |
| **__Perform the desired cell staining__** | **__Perform the desired cell staining__** | ||
prepare_samples.1705512812.txt.gz · Last modified: 2024/01/17 18:33 by flowcytometry
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