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newuser [2020/04/09 19:30]
flowcytometry
newuser [2024/01/26 18:21] (current)
flowcytometry
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-<​html><​img src="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=new_user.png"><​BR><​BR></​html>​+<​html><​img src="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=new_user_v2.png"><​BR><​BR></​html>​ 
 +===== Access to our resources ===== 
 +{{  spectral-flow-banner22.png}} 
 +To use our equipments, you must register on iMM's booking system (<​html><​a href="​https://​my.agendoscience.com/​calendar/?​id=NDFjYWxlbmRhcnM="​ target="​_blank">​Agendo</​a></​html>​).  
 +\\ 
 +Follow the instructions:​ for <​html><​a href="​https://​imm.medicina.ulisboa.pt/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=agendo_registration_instructions_for_immers_2024.pdf"​ target="​_blank">​iMMers</​a></​html>​ or for <​html><​a href="​https://​imm.medicina.ulisboa.pt/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=imm_agendo_registration_instructions_for_external_users_2024.pdf"​ target="​_blank">​external users</​a></​html>​.  
 +\\ 
 +You can ask permission to an equipment either by contacting us, or by clicking on the equipments’ calendar in Agendo. ​
  
-====== How to access our resources... ======+To access all systems (Cell Analysers, Cell Sorters and the Imaging Flow Cytometer) __you need to attend a 2 hour training session on the Principles of Flow Cytometry.__ Please book on the following link 
  
-==== If you want to use a cell analyzer: ====+[[Trainingprinciplesflow|{{principles_of_flow_cytometry.png}}]]
  
-  * Firstget in contact with us ({{telephone_icon.gif?​15}} 47224). We will give you a guided tour through ​the [[about|Flow Cytometry Facility]], so that you can have the best experience with our cytometers. +After this sessionwe will discuss experimental strategies and decide ​the most appropriate equipment ​for you.
-  * We strongly recomend you to read our tutorial ​for **[[https://​imm.medicina.ulisboa.pt/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=sop.ucf.002_-_basics_of_flow_cytometry.pdf|Basics of Flow Cytometry]]** and the **[[tutorials|e-learning courses]]** before your training session and before using the cytometers by yourself.  +
-  * You should definitely read the **[[usage_policy|Usage Rules]]** and detailed **[[https://​imm.medicina.ulisboa.pt/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=sop.ucf.004_-_ucf_imm_user_guidelines.pdf|User Guidelines]]** +
-  * The next step will be to use our **[[https://​imm.medicina.ulisboa.pt/​intranet/​booking|booking system]]** in order to request access to the flow cytometer ​you want to use (if you do not have access to the booking system yet, please contact us). +
-  * The [[about|Flow Cytometry Facility]] will schedule a training session with you, it is mandatory. Only after training you will get access to the booking system and the Flow Cytometry room.+
  
-==== If you want to use a cell sorter: ====+===Cell Analysers=== 
  
-  * Please ​[[imm-flowcytometry@medicina.ulisboa.pt|contact us]] ({{telephone_icon.gif?15}} 47224) to get access to the cell sorters online booking.+[[TrainingConventFlow|{{conventional_flow_cytometry.png}}]] 
 +\\ 
 +[[TrainingSpectralFlow|{{spectral_flow_cytometry.png}}]]
  
-==== If you want to analyze your results: ​==== +===Imaging Cytometer=== 
-  * We have ''​FlowJo'',​ ''​FACSDiva'',​ and ''​ModFit LT''​ software on our [[macflow|MacFlow]] and PC workstations. You can book the computers online, just like the rest of our instruments. We are happy to teach you how to draw gates, analyse your data, and create figures in FlowJo.+
  
-====== Important Information ======+[[TrainingImagingFlow|{{imaging_flow_cytometry.png}}]]
  
-  * **[[https://​imm.medicina.ulisboa.pt/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=sop.ucf.002_-_basics_of_flow_cytometry.pdf|Basics of Flow Cytometry]]** +===Flow Data Analysis===  
-  * **[[https://​imm.medicina.ulisboa.pt/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=sop.ucf.004_-_ucf_imm_user_guidelines.pdf|User Guidelines]]** + 
-  * **[[usage_policy|Usage Rules]]** +[[TrainingFlowData|{{flow_data_analysis.png}}]] 
-  * **[[prepare_samples|How to prepare samples for Flow Cytometry]]** + 
-  * **[[sorting_faq|What should I expect when sorting?]]**+===Cell Sorting===  
 + 
 +[[UCFServiceSorting|{{ucf_service_sorting.png}}]] 
 +\\ 
 +[[TrainingSelfSorting|{{self_cell_sorting.png}}]]
  
 ---- ----
 [[start|Back to the Flow Cytometry Wiki]] [[start|Back to the Flow Cytometry Wiki]]
newuser.1586453451.txt.gz · Last modified: 2020/04/09 19:30 by flowcytometry