imagestreamx
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This shows you the differences between two versions of the page.
| Both sides previous revision Previous revision Next revision | Previous revision Next revision Both sides next revision | ||
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imagestreamx [2018/06/08 18:32] flowcytometry |
imagestreamx [2024/01/22 18:39] flowcytometry |
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| <html> | <html> | ||
| - | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=imagestreamx_name.jpg"> | + | <img src="/facility/flowcytometry/lib/exe/fetch.php?media=amnis_nome.png"> |
| <table style="border:0px solid white;"> | <table style="border:0px solid white;"> | ||
| <tr style="border:0px solid white;"> | <tr style="border:0px solid white;"> | ||
| <td style="border:0px solid white;"> | <td style="border:0px solid white;"> | ||
| - | <a href="/facility/flowcytometry/lib/exe/fetch.php?media=imagestreamx_wiki.jpg"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=imagestreamx_wiki.jpg" width=300></a> | + | <a href="/facility/flowcytometry/lib/exe/fetch.php?media=imagestreamx_wiki.png"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=imagestreamx_wiki.png" width=300></a> |
| <td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
| <b>Location</b>: <a href="/facility/flowcytometry/lib/exe/fetch.php?media=flow_cytometry_map.jpg">Room P2-A-49</a> (<img src="/facility/flowcytometry/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47224) <br> | <b>Location</b>: <a href="/facility/flowcytometry/lib/exe/fetch.php?media=flow_cytometry_map.jpg">Room P2-A-49</a> (<img src="/facility/flowcytometry/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47224) <br> | ||
| - | <b>Manufacturer</b>: <a href="http://www.merckmillipore.com/PT/en" target="_blank">Merck</a><br> | + | <b>Manufacturer</b>: <a href="https://cytekbio.com/" target="_blank">Cytek</a><br> |
| - | <b>Model</b>: <a href="http://www.merckmillipore.com/PT/en/life-science-research/cell-analysis-flow-cytometry/amnis-imaging-flow-cytometers/imagestreamx-Mark-ii-imaging-flow-cytometer/VaSb.qB.QokAAAFLzRop.zHe,nav" target="_blank">Amnis ImageStreamX Mark II</a><br> | + | <b>Model</b>: <a href="https://cytekbio.com/pages/imagestream" target="_blank">Amnis ImageStreamX Mark II</a><br> |
| <b>Nickname</b>: "ImageStream"<br> | <b>Nickname</b>: "ImageStream"<br> | ||
| <b>Software</b>: INSPIRE 200.1.620.0 / IDEAS 6.2.64.0<br> | <b>Software</b>: INSPIRE 200.1.620.0 / IDEAS 6.2.64.0<br> | ||
| Line 16: | Line 16: | ||
|   <br> |   <br> | ||
|   <br> |   <br> | ||
| - |   <br> | + | → <a href="https://my.agendoscience.com/calendar/?id=MTBjYWxlbmRhcnM=" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=booking.png"> CYTEK Amnis ImageStreamX Booking</a> |
| - | → <a href="https://imm.medicina.ulisboa.pt/intranet/booking/145"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png"> Amnis ImageStreamX Booking</a> | + | <br> |
| + | → <a href="/facility/flowcytometry/doku.php?id=amnis_imagestreamx_usage"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=statistics.png"> CYTEK Amnis ImageStreamX Usage Statistics</a> | ||
| <br> | <br> | ||
| - | → <a href="/facility/flowcytometry/doku.php?id=amnis_imagestreamx_usage"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=chart_line.png"> Amnis ImageStreamX Usage Statistics</a> | + | → <a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=amnis_brochure.pdf"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=brochure.png"> CYTEK Amnis ImageStream Brochure</a> |
|   </p> |   </p> | ||
| </td> | </td> | ||
| Line 51: | Line 52: | ||
| ===== Image Acquisition Performance ===== | ===== Image Acquisition Performance ===== | ||
| - | {{::high_speed_imaging.png?700|}} | + | {{::high_speed_imaging.png?600|}} |
| ===== Additional information ===== | ===== Additional information ===== | ||
| * **[[prepare_samples|How to prepare samples for Flow Cytometry]]** | * **[[prepare_samples|How to prepare samples for Flow Cytometry]]** | ||
| - | * {{::imagestreamx_sample_preparation_guide.pdf| Sample Preparation Guide}}. **Note**: Samples are run using 1.5 ml microfuge tubes (Sarstedt ref. 72706) {{::microfuge_tube.jpg?60|}} | + | * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.026_-_amnis_imagestreamx_operation.pdf| Amnis ImageStreamX Operation]] |
| - | * {{::imagestreamx_brochure.pdf| ImageStreamX Brochure}} | + | * {{::imagestreamx_sample_preparation_guide.pdf| Sample Preparation Guide}}. **Note**: Samples are run using 1.5 ml microfuge tubes (Sarstedt ref. 72706) |
| ===== Booking Rules ===== | ===== Booking Rules ===== | ||
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| Please check the {{ ::sop.ucf.004_-_ucf_imm_user_guidelines.pdf |User Guidelines}} for detailed information. | Please check the {{ ::sop.ucf.004_-_ucf_imm_user_guidelines.pdf |User Guidelines}} for detailed information. | ||
| - | |||
| - | ===== Cleaning Protocol ===== | ||
| - | |||
| - | **Are you done?** | ||
| - | |||
| - | Just click on ''Shutdown''. The ImageStreamX will automatically turn off all illumination sources and rinse | ||
| - | the entire fluidic system with water, sterilizer, cleanser, de-bubbler, and then | ||
| - | water again. The sterilizer is held in the system for ten minutes to ensure decontamination. | ||
| - | |||
| - | You can walk away from the system. It takes about 45 minutes of unattended operation to complete. | ||
| ---- | ---- | ||
| [[resources|Back to the Resources page]] | [[resources|Back to the Resources page]] | ||
imagestreamx.txt · Last modified: 2024/01/22 18:42 by flowcytometry
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