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imagestreamx [2018/06/08 17:57]
flowcytometry
imagestreamx [2020/06/01 20:53]
flowcytometry
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 <tr style="​border:​0px solid white;">​ <tr style="​border:​0px solid white;">​
 <td style="​border:​0px solid white;">​ <td style="​border:​0px solid white;">​
-<a href="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=imagestreamx_wiki.jpg"><​img src="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=imagestreamx_wiki.jpg" width=300></​a>​+<a href="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=imagestreamx_wiki.png"><​img src="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=imagestreamx_wiki.png" width=300></​a>​
 <td style="​border:​0px solid white;">​ <p style="​line-height:​1.8">​ <td style="​border:​0px solid white;">​ <p style="​line-height:​1.8">​
 <​b>​Location</​b>:​ <a href="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=flow_cytometry_map.jpg">​Room P2-A-49</​a>​ (<img src="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=telephone_icon.gif"​ width=15>​ 47224) <​br> ​ <​b>​Location</​b>:​ <a href="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=flow_cytometry_map.jpg">​Room P2-A-49</​a>​ (<img src="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=telephone_icon.gif"​ width=15>​ 47224) <​br> ​
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 &nbsp <br> &nbsp <br>
 &nbsp <br> &nbsp <br>
-&rarr; <a href="​https://​imm.medicina.ulisboa.pt/​intranet/booking/145"><​img src="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=date.png"> ​ Amnis ImageStreamX Booking</​a>​+&rarr; <a href="​https://​my.agendo.science/calendar/?​id=MTBjYWxlbmRhcnM="><​img src="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=date.png"> ​ Amnis ImageStreamX Booking</​a>​
  <​br> ​  <​br> ​
 &rarr; <a href="/​facility/​flowcytometry/​doku.php?​id=amnis_imagestreamx_usage"><​img src="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=chart_line.png"> ​ Amnis ImageStreamX Usage Statistics</​a>​ &rarr; <a href="/​facility/​flowcytometry/​doku.php?​id=amnis_imagestreamx_usage"><​img src="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=chart_line.png"> ​ Amnis ImageStreamX Usage Statistics</​a>​
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 {{::​imagestreamx_fluorochrome_chart.png?​700|}} {{::​imagestreamx_fluorochrome_chart.png?​700|}}
 +
 +===== Objectives Information ​ =====
 +
 +^  Magnification ​ ^  20x  ^  40x  ^  60x  ^
 +^  Numerical Aperture ​ |  0.50  |  0.75  |  0.90  |
 +^  Field of View (μm)  |  120  |  60  |  40  |
 +^  Pixel Size (μm)  |  1.0  |  0.5  |  0.33  |
 +^  Depth of Field (μm)  |  8.0  |  4.0  |  2.5  |
 +
 +===== Image Acquisition Performance ===== 
 +
 +{{::​high_speed_imaging.png?​600|}}
  
 ===== Additional information ===== ===== Additional information =====
  
 +  * **[[prepare_samples|How to prepare samples for Flow Cytometry]]**
 +  * {{::​imagestreamx_sample_preparation_guide.pdf| Sample Preparation Guide}}. **Note**: Samples are run using 1.5 ml microfuge tubes (Sarstedt ref. 72706) {{::​microfuge_tube.jpg?​60|}}
   * {{::​imagestreamx_brochure.pdf| ImageStreamX Brochure}}   * {{::​imagestreamx_brochure.pdf| ImageStreamX Brochure}}
-  * {{::​imagestreamx_sample_preparation_guide.pdf| Sample Preparation Guide}}. **Note**: Samples are run using 1.5 ml microfuge tubes {{::​microfuge_tube.jpg?​60|}} 
  
 ===== Booking Rules ===== ===== Booking Rules =====
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 Please check the {{ ::​sop.ucf.004_-_ucf_imm_user_guidelines.pdf |User Guidelines}} for detailed information. Please check the {{ ::​sop.ucf.004_-_ucf_imm_user_guidelines.pdf |User Guidelines}} for detailed information.
 +
 +===== Cleaning Protocol =====
 +
 +**Are you done?** Just click on ''​Shutdown''​ and select ''​Shudown after sterilize''​. The ImageStreamX will automatically turn off all illumination sources and rinse
 +the entire fluidic system with water, sterilizer, cleanser, de-bubbler, and then
 +water again. The sterilizer is held in the system for ten minutes to ensure decontamination.
 +
 +You can walk away from the system. It takes about 45 minutes of unattended operation to complete.
  
 ---- ----
  
 [[resources|Back to the Resources page]] [[resources|Back to the Resources page]]
imagestreamx.txt · Last modified: 2021/11/18 22:57 by flowcytometry