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imagestreamx [2018/06/08 13:47]
flowcytometry
imagestreamx [2021/03/31 21:08]
flowcytometry
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 <tr style="​border:​0px solid white;">​ <tr style="​border:​0px solid white;">​
 <td style="​border:​0px solid white;">​ <td style="​border:​0px solid white;">​
-<a href="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=imagestreamx_wiki.jpg"><​img src="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=imagestreamx_wiki.jpg" width=300></​a>​+<a href="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=imagestreamx_wiki.png"><​img src="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=imagestreamx_wiki.png" width=300></​a>​
 <td style="​border:​0px solid white;">​ <p style="​line-height:​1.8">​ <td style="​border:​0px solid white;">​ <p style="​line-height:​1.8">​
 <​b>​Location</​b>:​ <a href="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=flow_cytometry_map.jpg">​Room P2-A-49</​a>​ (<img src="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=telephone_icon.gif"​ width=15>​ 47224) <​br> ​ <​b>​Location</​b>:​ <a href="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=flow_cytometry_map.jpg">​Room P2-A-49</​a>​ (<img src="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=telephone_icon.gif"​ width=15>​ 47224) <​br> ​
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 &nbsp <br> &nbsp <br>
 &nbsp <br> &nbsp <br>
-&rarr; <a href="​https://​imm.medicina.ulisboa.pt/​intranet/booking/145"><​img src="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=date.png"> ​ Amnis ImageStreamX Booking</​a>​+&rarr; <a href="​https://​my.agendo.science/calendar/?​id=MTBjYWxlbmRhcnM="><​img src="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=date.png"> ​ Amnis ImageStreamX Booking</​a>​
  <​br> ​  <​br> ​
 &rarr; <a href="/​facility/​flowcytometry/​doku.php?​id=amnis_imagestreamx_usage"><​img src="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=chart_line.png"> ​ Amnis ImageStreamX Usage Statistics</​a>​ &rarr; <a href="/​facility/​flowcytometry/​doku.php?​id=amnis_imagestreamx_usage"><​img src="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=chart_line.png"> ​ Amnis ImageStreamX Usage Statistics</​a>​
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   * **Multimag**:​ 20x, 40x and 60x magnification   * **Multimag**:​ 20x, 40x and 60x magnification
   * **Extended depth of field**: projects all structures within a cell into a single plane of focus   * **Extended depth of field**: projects all structures within a cell into a single plane of focus
 +
 +{{  ::​img-12channels.jpg ​ |}}
  
 ===== Configuration ===== ===== Configuration =====
  
-{{:bd_lsrfortessa_1_configuration_wiki.png?800|}}+{{::​imagestreamx_fluorochrome_chart.png?700|}} 
 + 
 +===== Objectives Information ​ ===== 
 + 
 +^  Magnification ​ ^  20x  ^  40x  ^  60x  ^ 
 +^  Numerical Aperture ​ |  0.50  |  0.75  |  0.90  | 
 +^  Field of View (μm)  |  120  |  60  |  40  | 
 +^  Pixel Size (μm)  |  1.0  |  0.5  |  0.33  | 
 +^  Depth of Field (μm)  |  8.0  |  4.0  |  2.5  | 
 + 
 +===== Image Acquisition Performance =====  
 + 
 +{{::​high_speed_imaging.png?​600|}} 
 + 
 +===== Additional information ===== 
 + 
 +  * **[[prepare_samples|How to prepare samples for Flow Cytometry]]** 
 +  * {{::​imagestreamx_sample_preparation_guide.pdf| Sample Preparation Guide}}. **Note**: Samples are run using 1.5 ml microfuge tubes (Sarstedt ref. 72706) {{::​microfuge_tube.jpg?​60|}} 
 +  * {{::​imagestreamx_brochure.pdf| ImageStreamX Brochure}}
  
 ===== Booking Rules ===== ===== Booking Rules =====
imagestreamx.txt · Last modified: 2024/01/22 18:42 by flowcytometry