Flow Cytometry Wiki

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<b>Location</b>: <a href="/facility/flowcytometry/lib/exe/fetch.php?media=flow_cytometry_map.jpg">Room P2-A-49</a> (<img src="/facility/flowcytometry/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47224) <br> 
<b>Manufacturer</b>: <a href="https://cytekbio.com/" target="_blank">Cytek</a><br>
<b>Model</b>: <a href="https://cytekbio.com/pages/aurora" target="_blank">Cytek Aurora</a><br>
<b>Nickname</b>: "Aurora"<br>
<b>Software</b>: SpectroFlo<br>
<b>Year</b>: 2023 <br>
<b>SN</b>:  <br>
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&rarr; <a href="https://my.agendoscience.com/calendar/?id=MTk3Y2FsZW5kYXJz"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png">  Cytek Aurora Booking</a>
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&rarr; <a href="/facility/flowcytometry/doku.php?id=cytek_aurora_usage"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=chart_line.png"> Cytek Aurora Usage Statistics</a> 
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{{:info.gif|}} **Next Scheduled Maintenances**:
===== System overview =====

{{  :optical_filters_aurora.png?0x250|}}
The Cytek Aurora leverages full spectrum technology to provide flexibility to scientists, enabling the use of a wide array of new fluorochrome combinations without reconfiguring the system for each application. The Aurora system delivers high-resolution data at the single-cell level to resolve the most challenging cell populations, such as cells with high autofluorescence or low levels of expression of key biomarkers, regardless of assay complexity.
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    * **5 spatially separated lasers**: Ultra-violet (355nm - 20mW), Violet (405nm - 100mW), Blue (488nm - 50mW), Yellow Green (561nm - 50mW) and Red (640nm - 80mW)
    
    * **3 scatter channels**: 488nm FSC, 405nm SSC, 488nm SSC
    
  * **64 fluorescent detectors**:
         * UV detector: 16 channels with optimized bandwidth from 365-829nm
         * V detector: 16 channels with optimized bandwidth from 420-829nm
         * B detector: 14 channels with optimized bandwidth from 498-829nm
         * YG detector: 10 channels with optimized bandwidth from 567-829nm
         * R detector: 8 channels with optimized bandwidth from 652-829nm

  * **Fluidics**
         * Vacuum-based fluidics system: manual acquisition from multiple tube formats
         * In-line flow meter: bead-less absolute cell count, bubble and clog detection
 
  * ** Automated Sample Loader (ASL)**
         * with an orbital shaker
         * compatible with __96-well plates__ , __96-deep well plates__ and __40-tube racks__


===== Configuration =====
{{:aurora_optical_configuration.png?900|}}
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===== Suggested Fluorochrome Peak Channels =====

{{{{:aurora_fluorophores.png?900|}}
{{{{:aurora_fluorophores_2.png?900|}}

===== Useful Links when Preparing your Experiment =====

  * [[prepare_samples|{{how_to_prepare_samples_for_flow_cytometry.png}}]]
  
  * <html><a href="https://spectrum.cytekbio.com/"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=cytek_spectrum_viewer.png" WIDTH=200></a></html>
  * <html><a href="https://cloud.cytekbio.com/login"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=cytek_cloud.png" WIDTH=300></a></html>

===== Additional Information =====

  * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.038_-_bd_facsymphony_a5_se_operation.pdf| Cytek Aurora Operation]]
  * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.022_-_bd_lsrfortessa_x-20_operation.pdf| SpectroFlo Tutorial]]	 
  * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.024_-_bd_lsrfortessa_facsymphony_a5_se_troubleshooting.pdf| Cytek Aurora Troubleshooting]]
  * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.042_-_cytek_aurora_cleaning.pdf| Cytek Aurora Cleaning]]
  * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.041_-cytek_aurora_starting_up_cleaning.pdf| Cytek Aurora Starting up Cleaning]] for the first user of the day, on weekends and out of staff working hours 

===== Booking Rules =====

Available 7 days a week, 24 hours a day for trained researchers. 

Please check the {{ ::sop.ucf.004_-_ucf_imm_user_guidelines.pdf |User Guidelines}} for detailed information.

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