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bd_lsr_fortessa_x20 [2023/11/02 17:14] flowcytometry |
bd_lsr_fortessa_x20 [2024/02/27 10:49] (current) flowcytometry |
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- | <a href="/facility/flowcytometry/lib/exe/fetch.php?media=fortessa_x20_wiki.png"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=fortessa_x20_wiki.png" width=300></a> | + | <a href="/facility/flowcytometry/lib/exe/fetch.php?media=fortessa_x20_wiki_2.jpg"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=fortessa_x20_wiki_2.jpg" width=300></a> |
<td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
<b>Location</b>: <a href="/facility/flowcytometry/lib/exe/fetch.php?media=flow_cytometry_map.jpg">Room P2-A-49</a> (<img src="/facility/flowcytometry/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47224) <br> | <b>Location</b>: <a href="/facility/flowcytometry/lib/exe/fetch.php?media=flow_cytometry_map.jpg">Room P2-A-49</a> (<img src="/facility/flowcytometry/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47224) <br> | ||
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  <br> |   <br> | ||
  <br> |   <br> | ||
- | → <a href="https://my.agendo.science/calendar/?id=NDJjYWxlbmRhcnM="><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png"> BD LSRFortessa X-20 Booking</a> | + | → <a href="https://my.agendoscience.com/calendar/?id=NDJjYWxlbmRhcnM=" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=booking.png"> BD LSRFortessa X-20 Booking</a> |
<br> | <br> | ||
- | → <a href="/facility/flowcytometry/doku.php?id=bd_lsrfortessa_x20_usage"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=chart_line.png"> BD LSRFortessa X-20 Usage Statistics</a> | + | → <a href="/facility/flowcytometry/doku.php?id=bd_lsrfortessa_x20_usage"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=statistics.png"> BD LSRFortessa X-20 Usage Statistics</a> |
+ | <br> | ||
+ | → <a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=bd_lsr_fortessax20_brochure.pdf" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=brochure.png"> BD LSRFortessa X-20 Brochure</a> | ||
</td> | </td> | ||
</tr> | </tr> | ||
</table> | </table> | ||
</html> | </html> | ||
- | {{:info.gif|}} **Next Scheduled Maintenances**: 19 January and 23 August | + | {{:info.gif|}} **Next Scheduled Maintenances**: 7 February and 21 August |
===== System overview ===== | ===== System overview ===== | ||
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* Four lasers with violet (405 nm), blue (488 nm), yellow-green (561 nm) and red (640 nm) | * Four lasers with violet (405 nm), blue (488 nm), yellow-green (561 nm) and red (640 nm) | ||
- | **If you need a high-throughput sampler, check the [[bd_lsr_fortessa_2|BD LSRFortessa 2]].** | + | **If you need multicolor analysis of more than 16 parameters, check the [[bd_facs_symphony_a5_se|BD FACSymphony A5 SE]] or the [[cytek_aurora|CYTEK Aurora]].** |
+ | |||
+ | **If you only need to analyse 13 or 4 parameters, check also the [[bd_lsr_fortessa_2|BD LSRFortessa]] and the [[bd_accuri_c6|BD Accuri C6 Plus]].** | ||
+ | |||
+ | **If you need a high-throughput sampler, check the [[bd_lsr_fortessa_2|BD LSRFortessa]] or the [[cytek_aurora|CYTEK Aurora]].** | ||
\\ | \\ | ||
- | **If you need a multicolor analysis of less then 16 parameters, check also [[bd_lsr_fortessa_1|BD LSRFortessa 1]], [[bd_lsr_fortessa_2|BD LSRFortessa 2]] or [[bd_accuri_c6|BD Accuri C6 Plus]] instead.** | ||
===== Configuration ===== | ===== Configuration ===== | ||
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\\ | \\ | ||
{{::bd_lsrfortessa_x-20_640.png?700|}} | {{::bd_lsrfortessa_x-20_640.png?700|}} | ||
+ | |||
+ | ===== Useful Links when Preparing your Experiment ===== | ||
+ | |||
+ | * [[prepare_samples|{{how_to_prepare_samples_for_flow_cytometry.png}}]] | ||
+ | * <html><a href="http://www.bdbiosciences.com/en-us/applications/research-applications/multicolor-flow-cytometry/product-selection-tools/spectrum-viewer" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_spectrum_viewer.png" WIDTH=300></a></html> | ||
+ | * <html><a href="http://www.bdbiosciences.com/en-us/applications/research-applications/multicolor-flow-cytometry/product-selection-tools/spectrum-viewer" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_research_cloud.png" WIDTH=300></a></html> | ||
===== Additional Information ===== | ===== Additional Information ===== | ||
- | * **[[prepare_samples|How to prepare samples for Flow Cytometry]]** | ||
* [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.022_-_bd_lsrfortessa_x-20_operation.pdf| BD LSRFortessa X-20 Operation]] | * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.022_-_bd_lsrfortessa_x-20_operation.pdf| BD LSRFortessa X-20 Operation]] | ||
+ | * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.003_-_bd_facsdiva_tutorial.pdf| BD FACSDiva Software Tutorial]] | ||
* [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.024_-_bd_lsrfortessa_troubleshooting.pdf| BD LSRFortessa Troubleshooting]] | * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.024_-_bd_lsrfortessa_troubleshooting.pdf| BD LSRFortessa Troubleshooting]] | ||
* [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.025_-_bd_lsrfortessa_cleaning.pdf| BD LSRFortessa Cleaning]] | * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.025_-_bd_lsrfortessa_cleaning.pdf| BD LSRFortessa Cleaning]] |