Flow Cytometry Wiki

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<b>Location</b>: <a href="/facility/flowcytometry/lib/exe/fetch.php?media=flow_cytometry_map.jpg">Room P2-A-49</a> (<img src="/facility/flowcytometry/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47224) <br> 
<b>Manufacturer</b>: <a href="http://www.bdbiosciences.com/eu/home" target="_blank">BD Biosciences</a><br>
<b>Model</b>: <a href="http://www.bdbiosciences.com/us/instruments/research/cell-analyzers/bd-lsrfortessa-x-20/m/1519232/overview" target="_blank">LSRFortessa X-20</a><br>
<b>Nickname</b>: "X20"<br>
<b>Software</b>: FACSDiva 8.0<br>
<b>Year</b>: 2017 <br>
<b>SN</b>: H65767SR1029 <br>
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&rarr; <a href="https://imm.medicina.ulisboa.pt/intranet/booking/144"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png">  BD LSRFortessa X-20 Booking</a>
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&rarr; <a href="/facility/flowcytometry/doku.php?id=bd_lsrfortessa_x20_usage"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=chart_line.png">  BD LSRFortessa X-20 Usage Statistics</a>
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&rarr; <a href="/facility/flowcytometry/doku.php?id=bd_lsrfortessa_x20_repair"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=repair-icon.gif" width=16> BD LSRFortessa X-20 Repair History</a></p>
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{{:info.gif|}} **Next Scheduled Maintenance**: August 7, 2018

===== System overview =====

{{  :cell_analyzers.png?0x300|}} The BD LSRFortessa X-20 //cell analyzer// is a benchtop digital flow cytometer equipped with a fixed-alignment flow cell that is gel-coupled to the collection optics. It has four spatially separated lasers - 405 nm, 488 nm, 561 nm and 640 nm. Its primary function is to characterize complex populations of cells. Individual cells pass single file through a stream, which is interrogated by the four lasers with the emitted light being detected by photomultiplier (PMT) tubes. The system electronics calculates the time it takes a single cell to pass through each laser intercept and assigns the signal of each PMT to the appropriate cell. Besides fluorescence, the physical properties of the cell are also analyzed with side scatter (SSC), which reflects the internal complexity of a cell and forward scatter (FSC), which is roughly proportional to cell size. The sample flow rate can be set to low, medium and high values, which correspond approximately to 12, 35 and 60 μl/minute, respectively. If you don't need multicolor analysis of up to 16 parameters, check the [[bd_lsr_fortessa_1|BD LSRFortessa 1]] instead. If you need a high-throughput sampler, check the [[bd_lsr_fortessa_2|BD LSRFortessa 2]].

  * Multicolor analysis of up to 16 parameters
  * Four lasers with violet (405 nm), blue (488 nm), yellow-green (561 nm) and red (640 nm)
===== Configuration =====

{{:bd_lsrfortessa_x-20_configuration_wiki_1.png?700|}}
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{{:bd_lsrfortessa_x-20_configuration_wiki_2.png?700|}}


===== Fluorophores Compatibility =====

{{{{:fortessa_x20_fluorochomes.png?700|}}
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{{:fortessa_x20_additional_filters.png?180|}}
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{{::bd_lsrfortessa_x-20_405.png?700|}}
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{{::bd_lsrfortessa_x-20_561.png?700|}}
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{{::bd_lsrfortessa_x-20_640.png?700|}}

===== Additional Information =====

  * **[[prepare_samples|How to prepare samples for Flow Cytometry]]**
  * [[https://imm.medicina.ulisboa.pt/files/1814/8700/1489/FORM.UCF.039_-_BD_LSRFortessa_X-20_operation.pdf| BD LSRFortessa X-20 Operation]]
  * [[https://imm.medicina.ulisboa.pt/files/5515/4827/1692/FORM.UCF.047_-_BD_LSRFortessa_troubleshooting.pdf| BD LSRFortessa Troubleshooting]]


===== Sample Preparation =====

  * [[http://static.bdbiosciences.com/documents/Flow-Cytometry-Reagents-PS-Panels-with-Fortessa-X20.pdf?_ga=1.148042303.945356473.1478101143|Multicolor Panels Designed for Optimal Resolution ]]
  * [[http://static.bdbiosciences.com/documents/X20-Protocol-15-Color-Immunophenotyping.pdf?_ga=1.82680222.945356473.1478101143|15-Color Immunophenotyping]]
  * [[http://static.bdbiosciences.com/documents/X20-Protocol-System-5-Color-Panels.pdf?_ga=1.82680222.945356473.1478101143|5-Color Panels Designed for Minimal Compensation]]
  * [[http://static.bdbiosciences.com/documents/X20-Protocol-4-Color-Panels-Minimal-Spectral-Overlap.pdf?_ga=1.111997196.945356473.1478101143|4-Color Panels Minimal Spectral Overlap]]
  * [[http://static.bdbiosciences.com/documents/X20-Protocol-8-Color-Panels-for-Mesenchymal-Stromal-Cells.pdf?_ga=1.111997196.945356473.1478101143|8-Color Panels for Mesenchymal Stromal Cells]]

===== Booking Rules =====

Available 7 days a week, 24 hours a day for trained researchers. 

Please check the {{ ::sop.ucf.004_-_ucf_imm_user_guidelines.pdf |User Guidelines}} for detailed information.

===== Cleaning Protocol =====

{{::cleaning_procedure_fortessa.png?700|}}

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