Flow Cytometry Wiki

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<b>Location</b>: <a href="/facility/flowcytometry/lib/exe/fetch.php?media=flow_cytometry_map.jpg">Room P2-A-49</a> (<img src="/facility/flowcytometry/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47224) <br> 
<b>Manufacturer</b>: <a href="http://www.bdbiosciences.com/eu/home" target="_blank">BD Biosciences</a><br>
<b>Model</b>: <a href="https://www.bdbiosciences.com/en-us/products/instruments/flow-cytometers/research-cell-analyzers/bd-lsrfortessa-x-20" target="_blank">LSRFortessa X-20</a><br>
<b>Nickname</b>: "X20"<br>
<b>Software</b>: FACSDiva 8.0<br>
<b>Year</b>: 2017 <br>
<b>SN</b>: H65767SR1029 <br>
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&rarr; <a href="https://my.agendoscience.com/calendar/?id=NDJjYWxlbmRhcnM=" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=booking.png">  BD LSRFortessa X-20 Booking</a>
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&rarr; <a href="/facility/flowcytometry/doku.php?id=bd_lsrfortessa_x20_usage"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=statistics.png">  BD LSRFortessa X-20 Usage Statistics</a> 
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&rarr; <a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=bd_lsr_fortessax20_brochure.pdf" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=brochure.png">  BD LSRFortessa X-20 Brochure</a>
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{{:info.gif|}} **Next Scheduled Maintenances**: 7 February  and 21 August
===== System overview =====

{{  :flow_cell_sit_sample.png?0x300|}} The BD LSRFortessa X-20 //cell analyzer// is a benchtop digital flow cytometer equipped with a fixed-alignment flow cell that is gel-coupled to the collection optics. It has four spatially separated lasers - 405 nm, 488 nm, 561 nm and 640 nm. Its primary function is to characterize complex populations of cells. Individual cells pass single file through a stream, which is interrogated by the four lasers with the emitted light being detected by photomultiplier (PMT) tubes. The system electronics calculates the time it takes a single cell to pass through each laser intercept and assigns the signal of each PMT to the appropriate cell. Besides fluorescence, the physical properties of the cell are also analyzed with side scatter (SSC), which reflects the internal complexity of a cell and forward scatter (FSC), which is roughly proportional to cell size. The sample flow rate can be set to low, medium and high values, which correspond approximately to 12, 35 and 60 μl/minute, respectively. 

  * Multicolor analysis of up to 16 parameters
  * Four lasers with violet (405 nm), blue (488 nm), yellow-green (561 nm) and red (640 nm)

**If you need multicolor analysis of more than 16 parameters, check the [[bd_facs_symphony_a5_se|BD FACSymphony A5 SE]] or the [[cytek_aurora|CYTEK Aurora]].**

**If you only need to analyse 13 or 4 parameters, check also the [[bd_lsr_fortessa_2|BD LSRFortessa]] and the [[bd_accuri_c6|BD Accuri C6 Plus]].**

**If you need a high-throughput sampler, check the [[bd_lsr_fortessa_2|BD LSRFortessa]] or the [[cytek_aurora|CYTEK Aurora]].**
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===== Configuration =====

{{:bd_lsrfortessa_x-20_configuration_new_.png?900|}}
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===== Fluorophores Compatibility =====

{{{{:fortessa_x20_fluorochromes.png?900|}}
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{{:fortessa_x20_additional_filters.png?250|}}
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{{::bd_lsrfortessa_x-20_405.png?700|}}
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{{::bd_lsrfortessa_x-20_488.png?700|}}
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{{::bd_lsrfortessa_x-20_561.png?700|}}
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{{::bd_lsrfortessa_x-20_640.png?700|}}

===== Useful Links when Preparing your Experiment =====

  * [[prepare_samples|{{how_to_prepare_samples_for_flow_cytometry.png}}]]
  * <html><a href="http://www.bdbiosciences.com/en-us/applications/research-applications/multicolor-flow-cytometry/product-selection-tools/spectrum-viewer" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_spectrum_viewer.png" WIDTH=300></a></html>
  * <html><a href="http://www.bdbiosciences.com/en-us/applications/research-applications/multicolor-flow-cytometry/product-selection-tools/spectrum-viewer" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_research_cloud.png" WIDTH=300></a></html>

===== Additional Information =====

  * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.022_-_bd_lsrfortessa_x-20_operation.pdf| BD LSRFortessa X-20 Operation]]
  * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.003_-_bd_facsdiva_tutorial.pdf| BD FACSDiva Software Tutorial]]
  * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.024_-_bd_lsrfortessa_troubleshooting.pdf| BD LSRFortessa Troubleshooting]]
  * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.025_-_bd_lsrfortessa_cleaning.pdf| BD LSRFortessa Cleaning]]
  * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.030_-_bd_lsrfortessa_X20_starting_up_cleaning.pdf| BD LSRFortessa X20 Starting up Cleaning]] for the first user of the day, on weekends and out of staff working hours 



===== Booking Rules =====

Available 7 days a week, 24 hours a day for trained researchers. 

Please check the {{ ::sop.ucf.004_-_ucf_imm_user_guidelines.pdf |User Guidelines}} for detailed information.

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