Flow Cytometry Wiki

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<b>Location</b>: <a href="/facility/flowcytometry/lib/exe/fetch.php?media=flow_cytometry_map.jpg">Room P2-A-49</a> (<img src="/facility/flowcytometry/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47224) <br> 
<b>Manufacturer</b>: <a href="http://www.bdbiosciences.com/eu/home" target="_blank">BD Biosciences</a><br>
<b>Model</b>: <a href="https://www.bdbiosciences.com/en-us/products/instruments/flow-cytometers/research-cell-analyzers/bd-lsrfortessa" target="_blank">LSRFortessa</a><br>
<b>Nickname</b>: "Fortessa 2"<br>
<b>Software</b>: FACSDiva 6.2<br>
<b>Year</b>: 2010 <br>
<b>SN</b>: H5B300009 <br>
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&rarr; <a href="https://my.agendoscience.com/calendar/?id=NDFjYWxlbmRhcnM=" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=booking.png">  BD LSRFortessa 2 Booking</a>
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&rarr; <a href="/facility/flowcytometry/doku.php?id=bd_lsrfortessa_2_usage"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=statistics.png">  BD LSRFortessa 2 Usage Statistics</a>
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&rarr; <a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=bd_lsrfortessa_brochure.pdf" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=brochure.png">  BD LSRFortessa 2 Brochure</a>
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{{:info.gif|}} **Next Scheduled Maintenances**: 22 June and 14 December

===== System overview =====

{{  :flow_cell_sit_sample.png?0x300|}} The BD LSRFortessa 2 //cell analyzer// is a benchtop digital flow cytometer equipped with a fixed-alignment flow cell that is gel-coupled to the collection optics. It has four spatially separated lasers - 405 nm, 488 nm, 561 nm and 640 nm. Its primary function is to characterize complex populations of cells. Individual cells pass single file through a stream, which is interrogated by the three lasers with the emitted light being detected by photomultiplier (PMT) tubes. The system electronics calculates the time it takes a single cell to pass through each laser intercept and assigns the signal of each PMT to the appropriate cell. Besides fluorescence, the physical properties of the cell are also analyzed with side scatter (SSC), which reflects the internal complexity of a cell and forward scatter (FSC), which is roughly proportional to cell size. The sample flow rate can be set to low, medium and high values, which correspond approximately to 12, 35 and 60 μl/minute, respectively. 

The LSRFortessa 2 is equipped with a **High Throughput Sampler (HTS)**, which allows the automated acquisition of samples from 96 and 384 well plates. 


  * Four lasers: violet (405 nm), blue (488 nm), yellow-green (561 nm) and red (640 nm)
  * Multicolor analysis of up to 13 parameters

**If you need multicolor analysis of up to 16 parameters, check the [[bd_lsr_fortessa_x20|BD LSRFortessa X-20]].**
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**If you only need to analyse 10 or 4 parameters, check also [[bd_lsr_fortessa_1|BD LSRFortessa 1]] and [[bd_accuri_c6|BD Accuri C6 Plus]].**


===== Configuration =====

{{:bd_lsrfortessa_2_configuration_(new).png?900|}}

===== Fluorophores Compatibility =====

{{:bd_lsrfortessa_2_fluorophores_wiki_new.png?900|}}
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{{::bd_lsrfortessa_2_additional_filters.png?360|}}
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{{::bd_lsrfortessa_2_405.png?700|}}
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{{::bd_lsrfortessa_x-20_488.png?700|}}
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{{::bd_lsrfortessa_x-20_561.png?700|}}
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{{::bd_lsrfortessa_2_640.png?700|}}

===== Useful Links when Preparing your Experiment =====

  * [[prepare_samples|{{how_to_prepare_samples_for_flow_cytometry.png}}]]
  * <html><a href="http://www.bdbiosciences.com/en-us/applications/research-applications/multicolor-flow-cytometry/product-selection-tools/spectrum-viewer" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_spectrum_viewer.png" WIDTH=300></a></html>
  * <html><a href="http://www.bdbiosciences.com/en-us/applications/research-applications/multicolor-flow-cytometry/product-selection-tools/spectrum-viewer" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_research_cloud.png" WIDTH=300></a></html>

===== Additional Information =====

  * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.021_-_bd_lsrfortessa_1_and_2_operation.pdf| BD LSRFortessa Operation]]
  * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.003_-_bd_facsdiva_tutorial.pdf| BD FACSDiva Software Tutorial]]
  * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.023_-_hts_operation_for_users.pdf| High Throughput Sampler (HTS) Operation]]
  * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.024_-_bd_lsrfortessa_troubleshooting.pdf| BD LSRFortessa Troubleshooting]]
  * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.025_-_bd_lsrfortessa_cleaning.pdf| BD LSRFortessa Cleaning]]
  * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.029_-_bd_lsrfortessa_1_2_starting_up_cleaning.pdf| BD LSRFortessa Starting up Cleaning]] for the first user of the day, on weekends and out of staff working hours 

===== Booking Rules =====

Available 7 days a week, 24 hours a day for trained researchers. 

Please check the {{ ::sop.ucf.004_-_ucf_imm_user_guidelines.pdf |User Guidelines}} for detailed information.

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