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bd_facsdiscover_s8

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Location: Lisbon Site, Room P2-A-49 ( 47224)
Manufacturer: BD Biosciences
Model: BD FACSDiscover S8
Nickname: "S8"
Software: BD FACSChorus
Year: 2024
SN:
 
 
 
BD FACSDiscover S8 Booking
BD FACSDiscover S8 Usage Statistics
BD FACSDiscover S8 Brochure 

Next Scheduled Maintenances:

Pseudomonas contamination timeline

System overview

The BD FACS Discover S8 is the first cell sorter that integrates imaging and full-spectrum capabilities. This versatile sorter offers both traditional sorting using standard fluorescent parameters, typical of conventional/full-spectrum sorters, and advanced imaging features, which can be visualized through histograms and bivariate plots. This significant innovation allows cells to be sorted not only based on the proteins they express but also by the spatial distribution of those proteins within the cell.

It has a new BD SpectralFX Technology that combines full-spectrum optics, next gen QC and system-aware spectral unmixing, managing spread by adapting to instrument performance and sample conditions in real time.

The BD CellView Image Technology, is the novel high-speed imaging technology that facilitates analysis and sorting through real-time integration of image and flow data.

  • Five lasers with ultra-violet (349nm), violet (405 nm), blue (488 nm), yellow-green (561 nm) and red (637 nm)
  • 78 fluorescent detectors
  • 3 scatter detectors (Blue 488nm laser):
    • FSC
    • SSC
    • Axial Light Loss (ALL)
  • 2 scatter detectors (Violet 405nm laser):
    • SSC
    • Axial Light Loss (ALL)

  • BD CellView Image Technology and BD SpectralFX Technology combining flow cytometry data with spatial and morphological insights.
  • Combines 15 image features with flow parameters for subset classification, statistical analysis and sorting
  • Resolves and provides image of 0.2-µm polystyrene particles from noise using at least one SSC parameter.
  • Sorting can be performed with speeds up to 12,000 events/second
  • 85 μm, 100 μm and 130 μm nozzles available
  • Up to 6-way sorting for 85 μm nozzle
  • Up to 4-way sorting for 100 μm nozzle
  • Up to 2-way sorting for 130 μm nozzle
  • Tube sorting for 1.5 mL eppendorfs and 5 mL tubes (FACS tubes)
  • Plate sorting for 6-, 24-, 96-, 384-well, microscope slides and other custom plates
  • The fully integrated Class II Type A2 biosafety cabinet meets personal and product protection, reducing the risk of operator exposure, while sorting potentially hazardous samples (e.g human samples and other BSL-2 level samples).


Configuration








Booking Rules

The system is available for booking from 10am to 6pm from Monday to Friday.

The cell sorting service is provided by a dedicated technician upon reservation on the online booking system. The system can be booked up to 1 months in advance.

Please check the User Guidelines for detailed information.


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bd_facsdiscover_s8.1736176670.txt.gz · Last modified: 2025/01/06 16:17 by flowcytometry