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bd_facsdiscover_s8

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Location: Lisbon Site, Room P2-A-49 ( 47224)
Manufacturer: BD Biosciences
Model: BD FACSDiscover S8
Nickname: "S8"
Software: BD FACSChorus
Year: 2024
SN:
 
 
 
BD FACSDiscover S8 Booking
BD FACSDiscover S8 Usage Statistics
BD FACSDiscover S8 Brochure 

Next Scheduled Maintenances:

Pseudomonas contamination timeline

System overview

The BD FACSDiscover™ S8 cell sorter is a high speed benchtop digital flow cytometer equipped with a sensitive, fixed-alignment gel-coupled cuvette flow cell. It has five spatially separated lasers - 349 nm, 405 nm, 488 nm, 561 nm and 637 nm. Its primary function is to analyze complex populations of cells and yield pure populations of cells meeting defined criteria. The sorter can purify samples that are simply positive and negative for a single fluorophore or as complex as multi-color samples with complex gating strategies.

The BD FACSDiscover™ S8 allows for high-parameter spectral flow cytometry. It enables you to gain full visible spectrum coverage to collect all light emitted without the need for filter changes, increasing dye flexibility and number of simultaneous colors. It also allows for autofluorescence unmixing, that may enable the resolution of difficult cells and populations. Its BD SpectralFX Technology combines full-spectrum optics, next gen QC and system-aware spectral unmixing that manages spread by adapting to instrument performance and sample conditions in real time.

The Cell Sorter leverages BD CellView Image Technology, a novel high-speed imaging technology that facilitates analysis and sorting through real-time integration of image and flow data.

The fully integrated Class II Type A2 biosafety cabinet meets personal and product protection, reducing the risk of operator exposure, while sorting potentially hazardous samples (e.g human samples and other BSL-2 level samples).

  • Five lasers with ultra-violet (349nm), violet (405 nm), blue (488 nm), yellow-green (561 nm) and red (637 nm)
  • 78 fluorescent detectors
  • 3 scatter detectors (Blue 488nm laser):
    • FSC
    • SSC
    • Axial Light Loss (ALL)
  • 2 scatter detectors (Violet 405nm laser):
    • SSC
    • Axial Light Loss (ALL)

  • BD CellView Image Technology and BD SpectralFX Technology combining flow cytometry data with spatial and morphological insights.
  • Combines 15 image features with flow parameters for subset classification, statistical analysis and sorting
  • Resolves and provides image of 0.2-µm polystyrene particles from noise using at least one SSC parameter.
  • Sorting can be performed with speeds up to 12,000 events/second
  • 85 μm, 100 μm and 130 μm nozzles available
  • Up to 6-way sorting for 85 μm nozzle
  • Up to 4-way sorting for 100 μm nozzle
  • Up to 2-way sorting for 130 μm nozzle
  • Tube sorting for 1.5 mL eppendorfs and 5 mL tubes (FACS tubes)
  • Plate sorting for 6-, 24-, 96-, 384-well, microscope slides and other custom plates


Configuration








Booking Rules

The system is available for booking from 10am to 6pm from Monday to Friday.

The cell sorting service is provided by a dedicated technician upon reservation on the online booking system. The system can be booked up to 1 months in advance.

Please check the User Guidelines for detailed information.


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bd_facsdiscover_s8.1736176333.txt.gz · Last modified: 2025/01/06 16:12 by flowcytometry