User Tools

Site Tools


bd_facsaria_iii

Differences

This shows you the differences between two versions of the page.

Link to this comparison view

Both sides previous revision Previous revision
Next revision
Previous revision
bd_facsaria_iii [2021/11/23 12:17]
flowcytometry
bd_facsaria_iii [2024/03/23 21:56] (current)
flowcytometry
Line 4: Line 4:
 <tr style="​border:​0px solid white;">​ <tr style="​border:​0px solid white;">​
 <td style="​border:​0px solid white;">​ <td style="​border:​0px solid white;">​
-<a href="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=aria_iii_wiki.png"><​img src="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=aria_iii_wiki.png" width=300></​a>​+<a href="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=aria_iii_wiki.png"><​img src="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=aria_iii_wiki_new.png" width=300></​a>​
 <td style="​border:​0px solid white;">​ <p style="​line-height:​1.8">​ <td style="​border:​0px solid white;">​ <p style="​line-height:​1.8">​
 <​b>​Location</​b>:​ <a href="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=flow_cytometry_map.jpg">​Room P2-A-49</​a>​ (<img src="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=telephone_icon.gif"​ width=15>​ 47224) <​br> ​ <​b>​Location</​b>:​ <a href="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=flow_cytometry_map.jpg">​Room P2-A-49</​a>​ (<img src="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=telephone_icon.gif"​ width=15>​ 47224) <​br> ​
Line 16: Line 16:
 &nbsp <br> &nbsp <br>
 &nbsp <br> &nbsp <br>
-&nbsp <​br>​ +&rarr; <a href="​https://​my.agendoscience.com/​calendar/?​id=NDVjYWxlbmRhcnM=" target="​_blank"><​img src="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=booking.png"> ​ BD FACSAria III Booking</​a>​
-&rarr; <a href="​https://​my.agendo.science/​calendar/?​id=NDVjYWxlbmRhcnM="><​img src="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=date.png"> ​ BD FACSAria III Booking</​a>​+
  <​br> ​  <​br> ​
-&rarr; <a href="/​facility/​flowcytometry/​doku.php?​id=bd_facsaria_iii_usage"><​img src="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=chart_line.png"> ​ BD FACSAria III Usage Statistics</​a>​+&rarr; <a href="/​facility/​flowcytometry/​doku.php?​id=bd_facsaria_iii_usage"><​img src="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=statistics.png"> ​ BD FACSAria III Usage Statistics</​a>​ 
 +<br>  
 +&rarr; <a href="​https://​imm.medicina.ulisboa.pt/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=bd-facsaria-iii-cell-sorter-brochure.pdf"​ target="​_blank"><​img src="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=brochure.png"> ​ BD FACSAria III brochure</a>
 &nbsp </p> &nbsp </p>
 </td> </td>
Line 25: Line 26:
 </​table>​ </​table>​
 </​html>​ </​html>​
-{{:​info.gif|}} **Next Scheduled Maintenances**: ​26 April and 18 October, 2021+{{:​info.gif|}} **Next Scheduled Maintenances**: ​April and 23 September
  
 {{:​info.gif|}} [[aria_iii_contamination|Pseudomonas contamination timeline]] {{:​info.gif|}} [[aria_iii_contamination|Pseudomonas contamination timeline]]
Line 34: Line 35:
  
 Sorting can be performed into two or four tubes with speeds up to 25,000 events/​second. The system sorts by incorporating cells from the sample tube into a stream of sterile PBS. The stream is interrogated by the lasers at the flow cell and the system electronics keeps track of each cell as they pass through the laser and determines specific cells that meet the sort criteria. A transducer vibrates the stream and induces droplet formation, with cells in the stream being incorporated into the droplets. If a cell meets the sorting criteria and is in the last drop before the break off, the instrument will charge that drop. The charged droplet is then deflected into the proper collection tube by the charge plates. Different cell types can be sorted with the use of 70 μm, 85 μm and 100 μm nozzles. Sorting can be performed into two or four tubes with speeds up to 25,000 events/​second. The system sorts by incorporating cells from the sample tube into a stream of sterile PBS. The stream is interrogated by the lasers at the flow cell and the system electronics keeps track of each cell as they pass through the laser and determines specific cells that meet the sort criteria. A transducer vibrates the stream and induces droplet formation, with cells in the stream being incorporated into the droplets. If a cell meets the sorting criteria and is in the last drop before the break off, the instrument will charge that drop. The charged droplet is then deflected into the proper collection tube by the charge plates. Different cell types can be sorted with the use of 70 μm, 85 μm and 100 μm nozzles.
- 
-**If you need to sort potentially hazardous samples (e.g human samples and other BSL-2 level samples) you need to sort in the [[bd_facsaria_fusion|BD FACSAria Fusion]], equiped with a Class II Type A2 biosafety cabinet to reduce the risk of operator exposure.**  ​ 
  
   * Four lasers - violet (405 nm), blue (488 nm), yellow-green (561 nm) and red (633 nm)   * Four lasers - violet (405 nm), blue (488 nm), yellow-green (561 nm) and red (633 nm)
   * Multicolor analysis of up to 16 parameters   * Multicolor analysis of up to 16 parameters
-  * 70 μm, 85 μm and 100 μm nozzles available+  * 70 μm, 85 μm100 μm and 130 μm nozzles available
   * Up to 4-way sorting into 1.5 ml or 4 ml tubes and 2-way sorting into 15 ml tubes   * Up to 4-way sorting into 1.5 ml or 4 ml tubes and 2-way sorting into 15 ml tubes
   * Sorting for 6-, 12-, 24-, 96-, 384-well and PCR plates   * Sorting for 6-, 12-, 24-, 96-, 384-well and PCR plates
 +
 +**If you need to sort potentially hazardous samples (e.g human samples and other BSL-2 level samples) you need to sort in the [[bd_facsaria_fusion|BD FACSAria Fusion]] or [[bd_facsymphony_s6_se|BD FACSymphony S6 SE]] equiped with a Class II Type A2 biosafety cabinet to reduce the risk of operator exposure.**  ​
 +
 +\\
 +**If you need high parameter cell sorting, check the [[bd_facsymphony_s6_se|BD FACSymphony S6 SE]].**
  
 ===== Configuration ===== ===== Configuration =====
  
-{{:​bd_facsaria_iii_configuration_new_.png?​1000|}}+{{:​bd_facsaria_iii_configuration_new_.png?​900|}}
  
  
Line 51: Line 55:
  
 {{:​facsaria_iii_fluorochrome_compatibility.png?​900|}} {{:​facsaria_iii_fluorochrome_compatibility.png?​900|}}
 +\\
 +{{:​aria_additional_filters.png?​300|}}
 \\ \\
 \\ \\
Line 61: Line 67:
 {{::​bd_facsaria_iii_633.png?​700|}} {{::​bd_facsaria_iii_633.png?​700|}}
  
-===== Additional Information ​=====+===== Useful Links when Preparing your Experiment ​=====
  
-  ​* **[[prepare_samples|How to prepare samples for Flow Cytometry]]**+  * [[prepare_samples|{{how_to_prepare_samples_for_flow_cytometry.png}}]] 
 +  ​<​html><​a href="​http://​www.bdbiosciences.com/​en-us/​applications/​research-applications/​multicolor-flow-cytometry/​product-selection-tools/​spectrum-viewer"​ target="​_blank"><​img src="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=bd_spectrum_viewer.png"​ WIDTH=300></​a></​html>​ 
 +  ​<​html><​a href="​http://​www.bdbiosciences.com/​en-us/​applications/​research-applications/​multicolor-flow-cytometry/​product-selection-tools/​spectrum-viewer"​ target="​_blank"><​img src="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=bd_research_cloud.png"​ WIDTH=300></​a></​html>​
  
 ===== Booking Rules ===== ===== Booking Rules =====
Line 76: Line 84:
 ---- ----
  
-[[resources|Back to the Resources ​page]]+[[resources|Back to the Equipment ​page]]
bd_facsaria_iii.1637666258.txt.gz · Last modified: 2021/11/23 12:17 by flowcytometry