This is an old revision of the document!
Location: Room P2-A-49 ( 47224)
The BD FACSAria III cell sorter is a high speed benchtop digital flow cytometer equipped with a sensitive, fixed-alignment cuvette flow cell. It has three spatially separated lasers - 488 nm, 561 nm and 633 nm. Its primary function is to analyze complex populations of cells and yield pure populations of cells meeting defined criteria. The sorter can purify samples that are simply positive and negative for a single fluorophore or as complex as multi-color samples with complex gating strategies. Sorting can be performed into two or four tubes with speeds up to 25,000 events/second. The system sorts by incorporating cells from the sample tube into a stream of sterile PBS. The stream is interrogated by the lasers at the flow cell and the system electronics keeps track of each cell as they pass through the laser and determines specific cells that meet the sort criteria. A transducer vibrates the stream and induces droplet formation, with cells in the stream being incorporated into the droplets. If a cell meets the sorting criteria and is in the last drop before the break off, the instrument will charge that drop. The charged droplet is then deflected into the proper collection tube by the charge plates. Different cell types can be sorted with the use of 70 μm, 85 μm and 100 μm nozzles. The instrument is housed in a Baker BioProtect-IV bio-safety cabinet and is able therefore to sort human samples. If you need a violet (405 nm) laser, check the BD FACSAria IIu instead.
The system is available for booking from 10am to 10pm from Monday to Friday.
The cell sorting service is provided by a dedicated technician upon reservation on the online booking system. The system can be booked up to 2 months in advance.
Please check the User Guidelines for detailed information.