Flow Cytometry Wiki

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<b>Location</b>: <a href="/facility/flowcytometry/lib/exe/fetch.php?media=flow_cytometry_map.jpg">Room P2-A-49</a> (<img src="/facility/flowcytometry/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47224) <br> 
<b>Manufacturer</b>: <a href="http://www.bdbiosciences.com/eu/home" target="_blank">BD Biosciences</a><br>
<b>Model</b>: <a href="http://www.bdbiosciences.com/us/instruments/research/cell-sorters/bd-facsaria-iii/m/744763/overview" target="_blank">FACSAria III</a><br>
<b>Nickname</b>: "Aria 3"<br>
<b>Software</b>: FACSDiva 6.1.3<br>
<b>Year</b>: 2011 <br>
<b>SN</b>: P28200134 <br>
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&rarr; <a href="https://imm.medicina.ulisboa.pt/intranet/booking/8"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=date.png">  BD FACSAria III Booking</a>
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&rarr; <a href="/facility/flowcytometry/doku.php?id=bd_facsaria_iii_usage"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=chart_line.png">  BD FACSAria III Usage Statistics</a>
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{{:info.gif|}} **Next Scheduled Maintenances**: 14th April and 13th October, 2020

{{:info.gif|}} [[aria_iii_contamination|Pseudomonas contamination timeline]]

===== System overview =====

{{  :cell_sorting.png?0x300|}} The BD FACSAria III //cell sorter// is a high speed benchtop digital flow cytometer equipped with a sensitive, fixed-alignment cuvette flow cell. It has three spatially separated lasers - 488 nm, 561 nm and 633 nm. Its primary function is to analyze complex populations of cells and yield pure populations of cells meeting defined criteria. The sorter can purify samples that are simply positive and negative for a single fluorophore or as complex as multi-color samples with complex gating strategies. Sorting can be performed into two or four tubes with speeds up to 25,000 events/second. The system sorts by incorporating cells from the sample tube into a stream of sterile PBS. The stream is interrogated by the lasers at the flow cell and the system electronics keeps track of each cell as they pass through the laser and determines specific cells that meet the sort criteria. A transducer vibrates the stream and induces droplet formation, with cells in the stream being incorporated into the droplets. If a cell meets the sorting criteria and is in the last drop before the break off, the instrument will charge that drop. The charged droplet is then deflected into the proper collection tube by the charge plates. Different cell types can be sorted with the use of 70 μm, 85 μm and 100 μm nozzles. **The instrument is housed in a Baker BioProtect-IV bio-safety cabinet** and is able therefore to sort human samples. If you need a violet (405 nm) laser, check the [[bd_facsaria_iiu|BD FACSAria IIu]] instead.

  * Three lasers with blue (488 nm), yellow-green (561 nm) and red (633 nm)
  * Multicolor analysis of up to 10 parameters
  * 70 μm, 85 μm and 100 μm nozzles available
  * Up to 4-way sorting into 1.5 ml or 4 ml tubes and 2-way sorting into 15 ml tubes
  * Sorting for 6-, 12-, 24-, 36-, 96-, 384-well and PCR plates

===== Configuration =====

{{:bd_facsaria_iii_configuration_wiki.png?800|}}

===== Fluorophores Compatibility =====

{{:bd_facsaria_iii_fluorophores_wiki.png?800|}}
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===== Additional Information =====

  * **[[prepare_samples|How to prepare samples for Flow Cytometry]]**
  * **[[sorting_faq|What should I expect when sorting?]]**

===== Booking Rules =====

The system is available for booking from 10am to 10pm from Monday to Friday. 

The cell sorting service is provided by a dedicated technician upon reservation on the online booking system. 
The system can be booked up to 2 months in advance. 

Please check the {{ ::sop.ucf.004_-_ucf_imm_user_guidelines.pdf |User Guidelines}} for detailed information.

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