Flow Cytometry Wiki

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<b>Location</b>: <a href="/facility/flowcytometry/lib/exe/fetch.php?media=flow_cytometry_map.jpg">Room P2-A-49</a> (<img src="/facility/flowcytometry/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47224) <br> 
<b>Manufacturer</b>: <a href="http://www.bdbiosciences.com/eu/home" target="_blank">BD Biosciences</a><br>
<b>Model</b>: <a href="https://www.bdbiosciences.com/en-us/products/instruments/flow-cytometers/research-cell-analyzers/bd-facsymphony-a5-se" target="_blank">FACSymphony A5 SE</a><br>
<b>Nickname</b>: "A5 SE"<br>
<b>Software</b>: FACSDiva 9.6<br>
<b>Year</b>: 2023 <br>
<b>SN</b>:  <br>
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&rarr; <a href="https://my.agendoscience.com/calendar/?id=MTk2Y2FsZW5kYXJz" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=booking.png">  BD FACSymphony A5 SE Booking</a>
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&rarr; <a href="/facility/flowcytometry/doku.php?id=bd_facsymphony_usage"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=statistics.png"> BD FACSymphony Usage Statistics</a> 
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&rarr; <a href="https://www.bdbiosciences.com/content/dam/bdb/marketing-documents/BD-FACSymphony-A5-SE-Brochure1.pdf" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=brochure.png">  BD FACSymphony A5 SE Brochure</a>
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{{:info.gif|}} **Next Scheduled Maintenances**:
===== System overview =====

{{  :flow_cell_sit_sample.png?0x300|}}
{{  :facsymphony_detectors.png?0x200|}} The BD FACSymphony™ A5 SE allows for high-parameter spectral flow cytometry. It enables you to gain full visible spectrum coverage to collect all light emitted without the need for filter changes, increasing dye flexibility and number of simultaneous colors. It also allows for autofluorescence unmixing, that may enable the resolution of difficult cells and populations.
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  * 48 fluorescent detectors
  * Five lasers with ultra-violet (349nm), violet (405 nm), blue (488 nm), yellow-green (561 nm) and red (640 nm) 
  * Enables both spectral unmixing and compensation workflows
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**If you need multicolor analysis of more than 48 parameters, check the [[cytek_aurora|CYTEK Aurora]].**

**If you need a high-throughput sampler, check the [[bd_lsr_fortessa_2|BD LSRFortessa 2]] or the [[cytek_aurora|CYTEK Aurora]].**

**If you need a multicolor analysis of less then 48 parameters, check also the [[bd_lsr_fortessa_X20|BD LSRFortessa X20]], the [[bd_lsr_fortessa_2|BD LSRFortessa 2]] or [[bd_accuri_c6|BD Accuri C6 Plus]].**
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===== Configuration =====
{{:facsymphony_optical_configuration.png?900|}}
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===== Suggested Fluorochrome Peak Channels =====

{{{{:facsymphony_fluorophores.png?900|}}
{{{{:facsymphony_fluorophores_2.png?900|}}

===== Useful Links when Preparing your Experiment =====

  * [[prepare_samples|{{how_to_prepare_samples_for_flow_cytometry.png}}]]
  * <html><a href="http://www.bdbiosciences.com/en-us/applications/research-applications/multicolor-flow-cytometry/product-selection-tools/spectrum-viewer" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_spectrum_viewer.png" WIDTH=300></a></html>
  * <html><a href="http://www.bdbiosciences.com/en-us/applications/research-applications/multicolor-flow-cytometry/product-selection-tools/spectrum-viewer" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_research_cloud.png" WIDTH=300></a></html>

===== Additional Information =====

  * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.038_-_bd_facsymphony_a5_se_operation.pdf| BD FACSymphony A5 SE Operation]]
  * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.037_-_bd_facsdiva_bd_facsymphony_a5_tutorial.pdf| BD FACSDiva Tutorial (for BD FACSymphony A5 SE)]]	 
  * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.024_-_bd_lsrfortessa_facsymphony_a5_se_troubleshooting.pdf| BD LSRFortessa & FACSymphony A5 SE Troubleshooting]]
  * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.040_-_bd_facsymphony_cleaning.pdf| BD FACSymphony A5 SE Cleaning]]
  * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.036_-_bd_facsymphony_a5_se_starting_up_cleaning.pdf| BD FACSymphony A5 SE Starting up Cleaning]] for the first user of the day, on weekends and out of staff working hours 

===== Booking Rules =====

Available 7 days a week, 24 hours a day for trained researchers. 

Please check the {{ ::sop.ucf.004_-_ucf_imm_user_guidelines.pdf |User Guidelines}} for detailed information.

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