Flow Cytometry Wiki

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<b>Location</b>: <a href="/facility/flowcytometry/lib/exe/fetch.php?media=flow_cytometry_map.jpg">Room P2-A-49</a> (<img src="/facility/flowcytometry/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47224) <br> 
<b>Manufacturer</b>: <a href="http://www.bdbiosciences.com/eu/home" target="_blank">BD Biosciences</a><br>
<b>Model</b>: <a href="https://www.bdbiosciences.com/en-us/products/instruments/flow-cytometers/research-cell-analyzers/bd-accuri-c6-plus" target="_blank">Accuri C6 Plus</a><br>
<b>Nickname</b>: "Accuri"<br>
<b>Software</b>: Accuri C6 Plus software 1.0.23.1<br>
<b>Year</b>: 2021 <br>
<b>SN</b>: AC66051710059 <br>
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&rarr; <a href="https://my.agendoscience.com/calendar/?id=NDNjYWxlbmRhcnM=" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=booking.png">  BD Accuri C6 Plus Booking</a>
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&rarr; <a href="/facility/flowcytometry/doku.php?id=bd_accuri_c6_usage"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=statistics.png">  BD Accuri C6 Plus Usage Statistics</a>
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===== System overview =====

{{  :accuri_optical_bench.png?0x300|}}The BD Accuri C6 Plus //cell analyzer// is an easy-to-use benchtop flow cytometer that allows for absolute cell counting and has a flexible optical layout. It has two lasers - 488 nm and 640 nm and four fluorescence detectors. The BD Accuri C6 Plus can acquire up to 10,000 events/second, with the data being digitally collected over a 7-decade dynamic range. Its low pressure pumping system allows for acquisition with any type of device (e.g. FACS, Eppendorf or Falcon tubes). Since the sample tube remains open (is not pressurized), the Accuri C6 Plus allows for addition of compounds without interruption in sampling.
**The fluidics system derives sample volume and can determine absolute cell counts** or sample concentration per microliter. 

  * Two lasers: blue (488 nm) and red (640 nm)
  * Multicolor analysis of up to 4 parameters

**If you need multicolor analysis of more than 4 parameters, check the [[bd_lsr_fortessa_2|BD LSRFortessa]], the [[bd_lsr_fortessa_x20|BD LSRFortessa X-20]], the [[bd_facs_symphony_a5_se|BD FACSymphony A5 SE]] or the [[cytek_aurora|CYTEK Aurora]].**

**If you need a high-throughput sampler, check the [[bd_lsr_fortessa_2|BD LSRFortessa]] or the [[cytek_aurora|CYTEK Aurora]]**.

===== Configuration =====

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===== Useful Links when Preparing your Experiment =====

  * [[prepare_samples|{{how_to_prepare_samples_for_flow_cytometry.png}}]]
  * <html><a href="http://www.bdbiosciences.com/en-us/applications/research-applications/multicolor-flow-cytometry/product-selection-tools/spectrum-viewer" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_spectrum_viewer.png" WIDTH=300></a></html>
  * <html><a href="http://www.bdbiosciences.com/en-us/applications/research-applications/multicolor-flow-cytometry/product-selection-tools/spectrum-viewer" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=bd_research_cloud.png" WIDTH=300></a></html>

===== Additional Information =====

  * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.019_-_bd_accuri_c6_plus_operation.pdf|BD Accuri C6 Plus Operation]]
  * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.011_-_bd_accuric6_plus_tutorial.pdf|BD Accuri C6 Plus Software Tutorial]]
  * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.021_-_bd_accuri_c6_plus_cleaning.pdf| BD Accuri C6 Plus Cleaning]]
  * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.031_-_accuri_c6_plus_starting_up_cleaning.pdf| BD Accuri C6 Plus Starting Up Cleaning]] for the first user of the day, on weekends and out of staff working hours 
===== Booking Rules =====

Available 7 days a week, 24 hours a day for trained researchers. 

Please check the [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.004_-_ucf_imm_user_guidelines.pdf |User Guidelines]] for detailed information.

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