bd_accuri_c6
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bd_accuri_c6 [2021/08/11 18:38] flowcytometry [Additional Information] |
bd_accuri_c6 [2021/11/23 13:01] flowcytometry |
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| ===== System overview ===== | ===== System overview ===== | ||
| - | The BD Accuri C6 Plus //cell analyzer// is an easy-to-use benchtop flow cytometer that allows for absolute cell counting and has a flexible optical layout. It has two lasers - 488 nm and 640 nm and four fluorescence detectors. The BD Accuri C6 Plus can acquire up to 10,000 events/second, with the data being digitally collected over a 7-decade dynamic range. Its low pressure pumping system allows for acquisition with any type of device (e.g. FACS, Eppendorf or Falcon tubes). Since the sample tube remains open (is not pressurized), the Accuri C6 Plus allows for addition of compounds without interruption in sampling. **The fluidics system derives sample volume and can determine absolute cell counts** or sample concentration per microliter. If you need a cell analyzer with more colors, check the [[bd_lsr_fortessa_1|BD LSRFortessa 1]] instead. | + | The BD Accuri C6 Plus //cell analyzer// is an easy-to-use benchtop flow cytometer that allows for absolute cell counting and has a flexible optical layout. It has two lasers - 488 nm and 640 nm and four fluorescence detectors. The BD Accuri C6 Plus can acquire up to 10,000 events/second, with the data being digitally collected over a 7-decade dynamic range. Its low pressure pumping system allows for acquisition with any type of device (e.g. FACS, Eppendorf or Falcon tubes). Since the sample tube remains open (is not pressurized), the Accuri C6 Plus allows for addition of compounds without interruption in sampling. **The fluidics system derives sample volume and can determine absolute cell counts** or sample concentration per microliter. |
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| + | **If you need a cell analyzer with more colors, check [[bd_lsr_fortessa_1|BD LSRFortessa 1]], [[bd_lsr_fortessa_2|BD LSRFortessa 2]] and [[bd_lsr_fortessa_x20|BD LSRFortessa X20]] instead.** | ||
| * Two lasers: blue (488 nm) and red (640 nm) | * Two lasers: blue (488 nm) and red (640 nm) | ||
| * Multicolor analysis of up to 4 parameters | * Multicolor analysis of up to 4 parameters | ||
| + | \\ | ||
| ===== Configuration ===== | ===== Configuration ===== | ||
| - | {{::accuri_default.png?620|}} | + | {{::accuri_default.png?800|}} |
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| + | {{::accuri_c6_plus_additional_filters.png?200}} | ||
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| {{::bd_accuri_c6_640.png?700|}} | {{::bd_accuri_c6_640.png?700|}} | ||
| - | Check [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=form.ucf.048_-_bd_accuri_c6_configurations.pdf|the list of possible configurations]]. | + | **Check [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=form.ucf.084_-_bd_accuri_c6_plus_laser_and_filters_configuration.pdf|the list of possible configurations]]** |
| ===== Additional Information ===== | ===== Additional Information ===== | ||
| * **[[prepare_samples|How to prepare samples for Flow Cytometry]]** | * **[[prepare_samples|How to prepare samples for Flow Cytometry]]** | ||
| - | * [[form.ucf.049_-_bd_accuri_c6_plus_operation.pdf| BD Accuri C6 Operation]] | + | * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=form.ucf.049_-_bd_accuri_c6_plus_operation.pdf|BD Accuri C6 Plus Operation]] |
| - | * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=form.ucf.071_-_bd_accuri_c6_cleaning.pdf| BD Accuri C6 Cleaning]] | + | * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=form.ucf.071_-_bd_accuri_c6_plus_cleaning.pdf| BD Accuri C6 Plus Cleaning]] |
| - | * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=form.ucf.082_-_accuri_starting_up_cleaning.pdf| BD Accuri C6 Starting Up Cleaning]] for the first user of the day, on weekends and out of staff working hours | + | * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=form.ucf.082_-_accuri_c6_plus_starting_up_cleaning.pdf| BD Accuri C6 Plus Starting Up Cleaning]] for the first user of the day, on weekends and out of staff working hours |
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| - | {{::form.ucf.049_-_bd_accuri_c6_plus_operation.pdf |User Guidelines}} | + | |
| ===== Booking Rules ===== | ===== Booking Rules ===== | ||
| Available 7 days a week, 24 hours a day for trained researchers. | Available 7 days a week, 24 hours a day for trained researchers. | ||
| - | Please check the {{::sop.ucf.004_-_ucf_imm_user_guidelines.pdf |User Guidelines}} for detailed information. | + | Please check the [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.004_-_ucf_imm_user_guidelines.pdf |User Guidelines]] for detailed information. |
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| [[resources|Back to the Resources page]] | [[resources|Back to the Resources page]] | ||
bd_accuri_c6.txt · Last modified: 2024/02/21 12:45 by flowcytometry
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