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about [2019/12/08 20:38] flowcytometry [Contacts] |
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| - | ====== About the Flow Cytometry Unit ====== | + | ====== About the Flow Cytometry Platform! ====== |
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| + | The Flow Cytometry Platform provides a state of the art flow cytometry service and expertise to GIMM researchers as well as external groups from other research institutes and companies.\\ | ||
| + | Laboratory staff is well trained in instrument operation, providing support to cell sorting, sample acquisition and data analysis. Training is available for all analytical instruments and SOP are implemented to comply with the highest quality standards required to ensure reproducibility in science.\\ | ||
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| + | If you want to purify a cell population in your sample, we can help you by providing a cell sorting service using 5 high speed cell sorters (BD FACSAria Fusion, BD FACSAria III,BD FACSAria IIu, BD FACSDiscover S8 and Cytek Aurora CS).\\ | ||
| - | The Flow Cytometry Unit provides a state of the art flow cytometry service to iMM researchers as well as external groups from other research institutes. We aim at training researchers in the adequate use of the instruments and also on flow cytometry principles, experiment planning, as well as data analysis and interpretation. The Flow Cytometry Facility also provides a cell sorting service using two High Speed Cell Sorters (BD FACSAria III and BD FACSAria IIu) operated by dedicated staff. | + | We are happy to help you with your flow cytometry experiments. Talk to us! We will give you a guided tour through our facility, so that you can have the best experience with us! |
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| - | ===== Team ===== | + | <html><td style="border:0px solid white;"> |
| - | + | <p style="text-align:left">                                                                      <img src="/facility/flowcytometry/lib/exe/fetch.php?media=services_new.png"> </p></td> | |
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| - | <div align="center"><b>Head of Facility</b></div> | + | |
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| - | <div style="width: 150px; height: 150px; overflow: hidden; position: relative;"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=jose_rino.png" style="width:150px; height: 150px;" /></div><br><br> | + | |
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| - | <b>José Rino</b> has a <i>Licenciatura</i> in Technological Physics Engineering (Instituto Superior Técnico) and a PhD in Biophysics (Faculdade de Ciências). José was appointed Head of the Flow Cytometry Unit in October 2016. He is also Head of the Bioimaging Unit at iMM. <a href="https://imm.medicina.ulisboa.pt/facility/bioimaging/doku.php?id=facility_bioimaging_doku.php&id=rino_cv">More information</a>.<br> | + | |
| - | <img src="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=mail_icon.png" width=20> | + | |
| - | <i>joserino@medicina.ulisboa.pt</i> | + | |
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| + | ===== Equipment ===== | ||
| - | <html> | + | Check our [[resources|Cell Analysers and Cell Sorters]]. |
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| - | <div align="center"><b>Technical Support</b></div> | + | |
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| - | <div style="width: 150px; height: 150px; overflow: hidden; position: relative;"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=andreia_santos.png" style="width:150px; height: 150px;" /></div><br><br> | + | |
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| - | <b>Andreia Santos</b> has a BSc in Biology and a MSc in Genetic and Molecular Biology from Faculdade de Ciências, Universidade de Lisboa. Andreia joined the Flow Cytometry Unit in September 2017 after working at Ana Espada de Sousa’s lab on human T-cell development, namely the role of IL-4 in the lineage plasticity of human CD4 single positive thymocytes.<br> | + | |
| - | <img src="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=mail_icon.png" width=20> | + | |
| - | <i>assantos@medicina.ulisboa.pt</i> | + | |
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| - | <div style="width: 150px; height: 150px; overflow: hidden; position: relative;"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=rute_goncalves_oficial.png" style="width:150px; height: 150px;" /></div><br><br> | + | |
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| - | <b>Rute Gonçalves</b> has a BSc in Biochemistry and a MSc in Molecular Genetics and Biomedicine from Universidade Nova de Lisboa. Rute joined the Flow Cytometry Unit in December 2018, after working at the Immunobiology and Pathogenesis Lab at CEDOC, under the supervision of Dra. Helena Soares, studying the main TLR4 signaling pathways in human T cells. She was also responsible for CEDOC’s Flow Cytometry Facility from April 2018 to October 2018, providing cell sorting services, supporting researchers in cell analyzers as well as software and data analysis and of course performing all the daily equipment maintenance and quality control. <br> | + | |
| - | <img src="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=mail_icon.png" width=20> | + | |
| - | <i>rute.goncalves@medicina.ulisboa.pt</i> | + | |
| - | </td> | + | |
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| - | </html> | + | |
| - | ===== Services ===== | + | ===== How to use the service ===== |
| - | Our flow cytometers are grouped into two categories: cell analyzers and cell sorters. Cell Analyzers are instruments for acquisition only, cells cannot be recovered once they are analyzed in the instrument. Cell sorters are capable of physically “sorting” out a population(s) of interest. | + | The Flow Cytometry Platform systems are available to GIMM researchers and external users. |
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| - | {{red-arrow-pointing-right.jpg|}} **More info on our** [[resources|{{flow_resources.jpg?0x30|}}]] | + | Follow the [[newuser|New User guidelines]] to start using our systems. |
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| - | {{::ucf_process.jpg|}} | + | Please read our [[usage_policy|Policies and Guidelines]] here. |
| ===== Prices ===== | ===== Prices ===== | ||
| + | Users are billed according to booked time and the actual time of usage. | ||
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| + | <html><a href="https://gimm.agendoscience.com/" target="_blank">Online booking</a></html> is mandatory for each usage session. | ||
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| + | **Cancellations and no shows will be charged extra**, please check the [[usage_policy|usage rules]] for more details.\\ | ||
| + | **Check our** [[billing|charge rates]]. | ||
| - | Users are billed for the actual time of usage. [[https://imm.medicina.ulisboa.pt/intranet/booking/select_resource.php?v=1|Online booking]] is mandatory for each usage session. **Cancellations and no shows will be charged extra**, please check the [[usage_policy|usage rules]] for more details. | + | ===== Service Hours ===== |
| - | {{red-arrow-pointing-right.jpg|}} **Check our** [[billing|{{charge_rates.jpg?0x30|}}]] | + | * **10 a.m. - 12 a.m.** and **2 p.m. - 5 p.m.** for **Training, Assistance and Consultations** |
| + | * **10 a.m. - 5pm (Oeiras Site) or 6pm (Lisbon Site)** for **Cell Sorting** | ||
| + | * **24 hours, 7 days a week** for **Equipment Usage** by authorized, trained users | ||
| - | ===== Hours ===== | ||
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| - | **9 a.m. - 6 p.m.** for **Training, Assistance and Consultations** | ||
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| - | 24 hours, 7 days a week for Equipment Usage by **authorized, trained users** (see [[usage_policy|Usage Rules]]) | ||
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| - | ===== How to use the service ===== | ||
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| - | All new users must contact the Flow Cytometry Unit to get proper training in using the systems and know the usage rules. Only after training will a user gain access to the Flow Cytometry room and to the online booking system. To get access to the cell sorters online booking, users must contact the Flow Cytometry staff. | ||
| - | Please read our policies and guidelines [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.004_-_ucf_imm_user_guidelines.pdf|here]]. | ||
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| - | {{:UCF_users_over_years.png?400|}} {{:UCF_usage_hours.png?400|}} \\ | ||
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| - | {{red-arrow-pointing-right.jpg|}} **Are you a** [[newuser|{{new_user.jpg?0x30|}}]] | ||
| ===== Contacts ===== | ===== Contacts ===== | ||
| - | Flow Cytometry Unit\\ | + | Flow Cytometry Platform\\ |
| - | **imm-flowcytometry@medicina.ulisboa.pt** | + | **flowcytometry@gimm.pt** |
| - | Instituto de Medicina Molecular, room P2-A-49\\ | + | <html><span style="color:Blueviolet;font-size:120%;">Lisbon site</span></html>\\ |
| - | Faculdade de Medicina de Lisboa \\ | + | Faculdade de Medicina de Lisboa\\ |
| + | Room P2-A-49 & P2-A-32 \\ | ||
| Av. Prof. Egas Moniz \\ | Av. Prof. Egas Moniz \\ | ||
| 1649-028 Lisboa \\ | 1649-028 Lisboa \\ | ||
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| - | {{flow_cytometry_map.jpg?700|}} | ||
| - | {{telephone_icon.gif?20|}}Internal phone extension: 47222 / 47224 | + | {{telephone_icon.gif?20|}}Internal phone extension: 47222 / 47224 External Tel: +351 217999530 |
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| + | <html><span style="color:Yellowgreen;font-size:120%;">Oeiras site</span></html>\\ | ||
| + | R. Q.ta Grande 6 \\ | ||
| + | Room 0B01 & 0B14, Bartolomeu Dias Wing\\ | ||
| + | 2780-156 Oeiras | ||
| + | {{telephone_icon.gif?20|}}Internal phone extension: 4625 External Tel: +351 214464625 | ||
| ---- | ---- | ||
| [[start|Back to the Flow Cytometry Wiki]] | [[start|Back to the Flow Cytometry Wiki]] | ||
about.1575833894.txt.gz · Last modified: 2019/12/08 20:38 by flowcytometry
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