User Tools

Site Tools


about

Differences

This shows you the differences between two versions of the page.

Link to this comparison view

Both sides previous revision Previous revision
Next revision
Previous revision
about [2019/04/13 18:39]
flowcytometry
about [2022/04/20 16:00] (current)
flowcytometry
Line 1: Line 1:
-====== About the Flow Cytometry ​Facility ​======+====== About the Flow Cytometry ​Unit! ====== 
 +\\  
 +{{gif_wiki_new_2021.gif }}  
 +\\ 
 +\\ 
 +\\ 
 +\\ 
 +The Flow Cytometry Unit provides a state of the art flow cytometry service to iMM researchers and visitors from other institutes. \\ 
 +We aim at training and supporting researchers in the adequate use of the instruments,​ on flow cytometry principles, experiment planning and sample preparation,​ as well as data analysis and interpretation. \\ \\ 
 +If you want to purify a cell population in your sample, we can help you by providing a cell sorting service using two high speed cell sorters (BD FACSAria Fusion and BD FACSAria III).\\ \\ 
 +We are happy to help you with your flow cytometry experiments. Talk to us! We will give you a guided tour through our facility, so that you can have the best experience with us!  
 +\\ 
 +\\ 
 +\\ 
 +\\ 
 +\\ 
 +\\
  
-The Flow Cytometry Facility provides a state of the art flow cytometry service to iMM Lisboa researchers as well as external groups from other research institutesWe aim at training researchers in the adequate use of the instruments ​and also on flow cytometry principles, experiment planning, as well as data analysis and interpretation. The Flow Cytometry Facility also provides a cell sorting service using two High Speed Cell Sorters ​(BD FACSAria III and BD FACSAria IIu) operated by dedicated staff.+===== Our Services =====   
 +{{  services_wiki_2.jpg?​900 ​ }} 
 +\\ 
 +Check our Analysers ​and Sorters ​ 
 +[[resources|{{equipment_v2.png?​120|}}]]
  
-===== Team =====+===== How to use the service ​=====
  
-<​html>​ +All new users must contact ​the Flow Cytometry ​Unit to get proper training ​in using the systems ​and know the usage rulesOnly after training will user gain access to the Flow Cytometry ​room and to the online booking systemTo get access to the cell sorters online bookingusers must contact ​the Flow Cytometry ​staff
-<table style="​border:​0px solid white;">​ +Please read our policies and guidelines [[https://​imm.medicina.ulisboa.pt/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=sop.ucf.004_-_ucf_imm_user_guidelines.pdf|here]].\\ 
-<td style="​border:​0px solid white;">​ +**Are you a ** [[newuser|{{new_user_v2.png?120}}]]
-<div align="​center"><​b>​Head of Facility</​b></​div>​ +
-<​br>​ +
-<div style="​width:​ 150px; height: 150px; overflow: hidden; position: relative;"><​img src="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=jose_rino.png"​ style="​width:​150px;​ height: 150px;"​ /></​div><​br><​br>​ +
-</​td>​ +
-<td style="​border:​0px solid white;">​  +
-<span style="​line-height:​1.8">​ +
-<​br>​ +
-<​br>​ +
-<​b>​José Rino</​b>​ has a <​i>​Licenciatura</​i>​ in Technological Physics Engineering (Instituto Superior Técnico) and a PhD in Biophysics (Faculdade de Ciências). José was appointed Head of the Flow Cytometry ​Facility ​in October 2016. He is also Head of the Bioimaging Facility at iMM. <a href="​https://​imm.medicina.ulisboa.pt/​facility/​bioimaging/​doku.php?​id=facility_bioimaging_doku.php&​id=rino_cv">​More information</​a>​.<​br>​ +
-<img src="​https://​imm.medicina.ulisboa.pt/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=mail_icon.png"​ width=20>​  +
- <​i>​joserino@medicina.ulisboa.pt</​i>​ +
-</​td>​ +
-</​table>​ +
-</​html>​ +
- +
-<​html>​ +
-<table style="​border:​0px solid white;">​ +
-<tr style="​border:​0px solid white;">​ +
-<td style="​border:​0px solid white;">​ +
-<div align="​center"><​b>​Technical Support</​b></​div>​ +
-<​br>​ +
-<div style="​width:​ 150px; height: 150px; overflow: hidden; position: relative;"><​img src="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=andreia_santos.png"​ style="​width:​150px;​ height: 150px;"​ /></​div><​br><​br>​ +
-</​td>​ +
-<td style="​border:​0px solid white;">​  +
-<span style="​line-height:​1.8">​ +
-<​br>​ +
-<​br>​ +
-<​b>​Andreia Santos</​b>​ has a BSc in Biology ​and a MSc in Genetic and Molecular Biology from Faculdade de CiênciasAndreia joined the Flow Cytometry Facility in September 2017 after working at Ana Espada de Sousa’s lab on human T-cell development,​ namely the role of IL-4 in the lineage plasticity of human CD4 single positive thymocytes.<​br>​ +
-<img src="​https://​imm.medicina.ulisboa.pt/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=mail_icon.png"​ width=20>​  +
- <​i>​assantos@medicina.ulisboa.pt</​i>​ +
-</​td>​ +
-</​tr>​ +
-<tr style="​border:​0px solid white;">​ +
-<td style="​border:​0px solid white;">​ +
-<div style="​width:​ 150px; height: 150px; overflow: hidden; position: relative;"><​img src="/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=rute_goncalves.png"​ style="​width:​150px;​ height: 150px;"​ /></​div><​br><​br>​ +
-</​td>​ +
-<td style="​border:​0px solid white;">​  +
-<span style="​line-height:​1.8">​ +
-<​b>​Rute Gonçalves</​b>​ has BSc in Biochemistry and a MSc in Molecular Genetics and Biomedicine from Universidade Nova de Lisboa. Rute joined ​the Flow Cytometry ​Facility in December 2018, after working at the Immunobiology ​and Pathogenesis Lab at CEDOC, under the supervision of DraHelena Soaresstudying ​the main TLR4 signaling pathways in human T cells. She was also responsible for CEDOC’s ​Flow Cytometry ​Facility from April 2018 to October 2018, providing cell sorting services, supporting researchers in cell analyzers as well as software and data analysis and of course performing all the daily equipment maintenance and quality control<br> +
-<img src="https://​imm.medicina.ulisboa.pt/​facility/​flowcytometry/​lib/​exe/​fetch.php?​media=mail_icon.png" width=20>​  +
- <​i>​rute.goncalves@medicina.ulisboa.pt</​i>​ +
-</​td>​ +
-</​tr>​ +
-</​table>​ +
-</​html>​ +
- +
-===== Services =====   +
- +
-Our flow cytometers are grouped into two categories: cell analyzers and cell sorters. Cell Analyzers are instruments for acquisition only, cells cannot be recovered once they are analyzed in the instrument. Cell sorters are capable of physically “sorting” out a population(s) of interest. +
- +
-{{red-arrow-pointing-right.jpg|}} ​**More info on our** [[resources|{{flow_resources.jpg?0x30|}}]] +
- +
-{{::​ucf_process.jpg|}}+
  
 ===== Prices ===== ===== Prices =====
 +Users are billed for the actual time of usage. [[https://​imm.agendo.science|Online booking]] is mandatory for each usage session. **Cancellations and no shows will be charged extra**, please check the [[usage_policy|usage rules]] for more details.\\
 +**Check our** [[billing|{{charges_v2.png?​120}}]]
  
-Users are billed for the actual time of usage. [[https://​imm.medicina.ulisboa.pt/​intranet/​booking/​select_resource.php?​v=1|Online booking]] is mandatory for each usage session. **Cancellations and no shows will be charged extra**, please check the [[usage_policy|usage rules]] for more details. +===== Service ​Hours =====
- +
-{{red-arrow-pointing-right.jpg|}} **Check our** [[billing|{{charge_rates.jpg?​0x30|}}]] +
- +
-===== Hours =====+
  
 **9 a.m. - 6 p.m.** for **Training, Assistance and Consultations** **9 a.m. - 6 p.m.** for **Training, Assistance and Consultations**
- 
 24 hours, 7 days a week for Equipment Usage by **authorized,​ trained users** (see [[usage_policy|Usage Rules]]) 24 hours, 7 days a week for Equipment Usage by **authorized,​ trained users** (see [[usage_policy|Usage Rules]])
- 
-===== How to use the service ===== 
- 
-All new users must contact the Flow Cytometry Facility to get proper training in using the systems and know the usage rules. Only after training will a user gain access to the Flow Cytometry room and to the online booking system. To get access to the cell sorters online booking, users must contact the Flow Cytometry staff. 
-Please read our policies and guidelines [[https://​imm.medicina.ulisboa.pt/​files/​6714/​6348/​2676/​SOP.UCF.004_-_UCF_IMM_USER_GUIDELINES.pdf|here]]. 
- 
-{{:​UCF_users_over_years.png?​400|}} {{:​UCF_usage_hours.png?​400|}} \\ 
- 
-{{red-arrow-pointing-right.jpg|}} **Are you a** [[newuser|{{new_user.jpg?​0x30|}}]] 
  
 ===== Contacts ===== ===== Contacts =====
  
-Flow Cytometry ​Facility\\+Flow Cytometry ​Unit\\
 **imm-flowcytometry@medicina.ulisboa.pt** **imm-flowcytometry@medicina.ulisboa.pt**
  
about.1555173585.txt.gz · Last modified: 2019/04/13 18:39 by flowcytometry