Location: Room P2-A-49 ( 47224)
Manufacturer: Cytek
Model: Cytek Aurora
Nickname: "Aurora"
Software: SpectroFlo
Year: 2023
SN:U1378 + FG148 (Loader ASL)
 
 
 
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{{:info.gif|}} **Next Scheduled Maintenances**: ===== System overview ===== {{ :optical_filters_aurora.png?0x250|}} The Cytek Aurora leverages full spectrum technology to provide flexibility to scientists, enabling the use of a wide array of new fluorochrome combinations without reconfiguring the system for each application. The Aurora system delivers high-resolution data at the single-cell level to resolve the most challenging cell populations, such as cells with high autofluorescence or low levels of expression of key biomarkers, regardless of assay complexity. \\ \\ * **5 spatially separated lasers**: Ultra-violet (355nm - 20mW), Violet (405nm - 100mW), Blue (488nm - 50mW), Yellow Green (561nm - 50mW) and Red (640nm - 80mW) * **3 scatter channels**: 488nm FSC, 405nm SSC, 488nm SSC * **64 fluorescent detectors**: * UV detector: 16 channels with optimized bandwidth from 365-829nm * V detector: 16 channels with optimized bandwidth from 420-829nm * B detector: 14 channels with optimized bandwidth from 498-829nm * YG detector: 10 channels with optimized bandwidth from 567-829nm * R detector: 8 channels with optimized bandwidth from 652-829nm * **Fluidics** * Vacuum-based fluidics system: manual acquisition from multiple tube formats * In-line flow meter: bead-less absolute cell count, bubble and clog detection * ** Automated Sample Loader (ASL)** * with an orbital shaker * compatible with __96-well plates__ , __96-deep well plates__ and __40-tube racks__ \\ **If you need high parameter cell analysis, check the [[bd_facs_symphony_a5_se|BD FACSymphony A5 SE]].** **If you need a multicolor analysis of less then 16, 13 or 4 parameters, check also the [[bd_lsr_fortessa_X20|BD LSRFortessa X20]], the [[bd_lsr_fortessa_2|BD LSRFortessa]] or [[bd_accuri_c6|BD Accuri C6 Plus]].** ===== Configuration ===== {{:aurora_optical_configuration.png?900|}} \\ \\ ===== Suggested Fluorochrome Peak Channels ===== {{{{:aurora_fluorophores.png?900|}} {{{{:aurora_fluorophores_2.png?900|}} ===== Useful Links when Preparing your Experiment ===== * [[prepare_samples|{{how_to_prepare_samples_for_flow_cytometry.png}}]] * * ===== Additional Information ===== * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.050-_cytek_aurora_operation.pdf| Cytek Aurora Operation]] * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.020_-_bd_lsrfortessa_x-20_operation.pdf| SpectroFlo Tutorial]] * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.020_-_bd_lsrfortessa_facsymphony_a5_se_troubleshooting.pdf| Cytek Aurora Troubleshooting]] * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.042_-_cytek_aurora_cleaning.pdf| Cytek Aurora Cleaning]] * [[https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.041_-cytek_aurora_starting_up_cleaning.pdf| Cytek Aurora Starting up Cleaning]] for the first user of the day, on weekends and out of staff working hours ===== Booking Rules ===== Available 7 days a week, 24 hours a day for trained researchers. Please check the {{ ::sop.ucf.004_-_ucf_imm_user_guidelines.pdf |User Guidelines}} for detailed information. ---- [[resources|Back to the Equipment page]]