GIMM Flow Wiki

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<b>Location</b>: Lisbon Site, P2-A-32 <br>(<img src="/facility/flowcytometry/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47222)<br> 
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Flow cytometry data analysis can be performed either __on-site at the **Foxtrot workstation** (available to all users)__ or __**remotely** (restricted to Lisbon GIMM users)__. 
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In all cases, **analysis is conducted within a Mambo Area**, where the required software is available (see table below). 
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Remote users must reserve a Mambo Area via the Agendo Calendar, while on-site users are required to book both the Foxtrot workstation and a Mambo Area. Agendo Calendar links are provided in the table below. 
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For detailed instructions on remote access to the Mambo Workstation, please refer to the following document <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.flow.996_-_mambo_remote_workstation_.pdf" target="_blank"> SOP.FLOW.996 - Mambo Remote Workstation</a></html>.
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^ Site      ^ Physical Workstation                  ^ Working Area          ^ Software          ^
| Lisbon    | <html><a href="https://europe.agendoscience.com/calendar/?id=NDUwY2FsZW5kYXJz" target="_blank">Foxtrot</a></html> | <html><a href="https://europe.agendoscience.com/calendar/?id=NDQ1Y2FsZW5kYXJz" target="_blank">MamboUser1</a></html>        | SpectroFlo        |
| :::    | :::                        | <html><a href="https://europe.agendoscience.com/calendar/?id=NDQ2Y2FsZW5kYXJz&date=07042026" target="_blank">MamboUser2</a></html>        | SpectroFlo        |
| :::    | :::                        | <html><a href="https://europe.agendoscience.com/calendar/?id=NDQ3Y2FsZW5kYXJz" target="_blank">MamboUser3</a></html>        | SpectroFlo and FlowJo        |
| :::    | ::: | <html><a href="https://europe.agendoscience.com/calendar/?id=NDQ4Y2FsZW5kYXJz" target="_blank">VMMamboUser1</a></html>        | FlowJo        |
| :::    | :::                        | <html><a href="https://europe.agendoscience.com/calendar/?id=NDQ5Y2FsZW5kYXJz&date=07042026" target="_blank">VMMamboUser2</a></html>        | FlowJo, Cell Image Extractor and CellView Lens plugin      |
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<b>Location</b>: Oeiras Site, Bartolomeu Dias Wing,<br>(<img src="/facility/flowcytometry/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 4625)<br> 
<b>Software</b>: SpectroFlo, FlowJo, IDEAS<br>
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The Moonwalk is a high-performance data analysis workstation dedicated to analysis of spectral flow cytometry data with **SpectroFlo**. 
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The workstation is also equipped with **FlowJo Software** with a shared license from the Flow Platform, and the **IDEAS software 6.2** to analyse your data from the [[imagestreamx|Amnis Image Stream]]. 
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It is available free of charge, but please remember to book your session in advance through the <html><a href="https://europe.agendoscience.com/calendar/?id=MTljYWxlbmRhcnM=" target="_blank">Moonwalk Agendo system</a></html>.
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If you need to analyse your data in FlowJo, IDEAS software 6.4 and Machine Learning **remotely**, use the **Tango Workstation**. 
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Tango is a **remote** high-performance data analysis workstation and it is available free of charge.

If you have IGC credentials, you have access to **FlowJo Software** and also to **IDEAS software** and **Machine Learning** to analyse data from the Amnis ImageStream. Please remember to book your session in advance through the <html><a href="https://europe.agendoscience.com/calendar/?id=MThjYWxlbmRhcnM=" target="_blank">Tango Agendo system - Oeiras Users</a></html>

For users at the Lisbon site, access is limited to **IDEAS software 6.4** and **Machine Learning** to analyse data from the Amnis Image Stream. Please remember to book your session in advance through the <html><a href="https://europe.agendoscience.com/calendar/?id=NDIxY2FsZW5kYXJz" target="_blank">Tango Agendo system - Lisbon Site and External Users</a></html>

For external users, access to Tango workstation is only possible using the **Moonwalk workstation**. 

Remote users must reserve a Tango via the Agendo Calendar, while on-site users are required to book both the Moonwalk workstation and a Tango.

For detailed instructions on remote access to the Tango Workstation, please refer to the following document <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.flow.999_-_tango_remote_workstation_v2.pdf" target="_blank"> SOP.FLOW.999 - Tango Remote Workstation</a></html>.
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<b>Location</b>: Oeiras Site, Bartolomeu Dias Wing, <br>(<img src="/facility/flowcytometry/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 4625)<br> 
<b>Software</b>: FACSChorus, FlowJo
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The Samba is a high-performance data analysis workstation dedicated to the analysis of **BD FACSDiscover S8** flow cytometry data generated in BD **FACSChorus**. It provides the FACSChorus, enabling data analysis and experimental troubleshooting off-instrument, as well as the **Cell View Extractor Software**, **FlowJo** and the **Cell View Lens Plugin** for advanced analysis of images generated by the instrument.

It is available free of charge, but please remember to book your session in advance through the <html><a href="https://europe.agendoscience.com/calendar/?id=NDc4Y2FsZW5kYXJz" target="_blank">Samba Agendo system</a></html>.

===== Useful Links =====  
  * <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.049_spetroflo_cytek_aurora_tutorial_users.pdf" target="_blank">SpectroFlo Tutorial</a></html>
  * <html><a href="https://www.flowjo.com/solutions/flowjo/downloads/tutorials" target="_blank"> FlowJo Tutorials</a></html>
  * <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=flowjo_basic_tutorial_2018.pdf" target="_blank"> FlowJo Basic Tutorial </a></html>
  * <html><a href="https://www.medizin.uni-tuebingen.de/files/download/8YWbqX1VelmV0EKRLxkr9apM/IDEAS-User-Manual_6.2.pdf" target="_blank">IDEAS Software User Manual</a></html>


===== Former Workstations =====

[[pc|PC Workstation]]

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