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=====Lisbon Workstation=====
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{{workstations_lisbon_site.png?500}}
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Flow cytometry data analysis can be performed either __on-site at the **Foxtrot workstation**__ or __remotely (restricted to Lisbon GIMM users)__. 
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In all cases, **analysis is conducted within a Mambo Area**, where the required software is available (see table below). 
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Remote users must reserve a Mambo Area via the Agendo Calendar, while on-site users are required to book both the Foxtrot workstation and a Mambo Area. Agendo Calendar links are provided in the table below. 
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For detailed instructions on remote access to the Mambo Workstations, please refer to the following document <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.flow.996_-_mambo_remote_workstation_.pdf" target="_blank"> SOP.FLOW.996 - Mambo Remote Workstation</a></html>.
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^ Site      ^ Physical Workstation                  ^ Working Area          ^ Software          ^
| Lisbon    | [[https://europe.agendoscience.com/calendar/?id=NDUwY2FsZW5kYXJz|Foxtrot]] | [[https://europe.agendoscience.com/calendar/?id=NDQ1Y2FsZW5kYXJz|MamboUser1]]        | SpectroFlo        |
| :::    | :::                        | [[https://europe.agendoscience.com/calendar/?id=NDQ2Y2FsZW5kYXJz&date=07042026|MamboUser2]]        | SpectroFlo        |
| :::    | :::                        | [[https://europe.agendoscience.com/calendar/?id=NDQ3Y2FsZW5kYXJz|MamboUser3]]        | SpectroFlo and FlowJo        |
| :::    | ::: | [[https://europe.agendoscience.com/calendar/?id=NDQ4Y2FsZW5kYXJz|VMMamboUser1]]        | FlowJo        |
| :::    | :::                        | [[https://europe.agendoscience.com/calendar/?id=NDQ5Y2FsZW5kYXJz&date=07042026|VMMamboUser2]]        | FlowJo and CellView        |

<html>
<img src="/facility/flowcytometry/lib/exe/fetch.php?media=moonwalk_name_wiki.png">
<table style="border:0px solid white;">
<tr style="border:0px solid white;">
<td style="border:0px solid white;">
<img src="/facility/flowcytometry/lib/exe/fetch.php?media=moonwalk_wiki.png"WIDTH=300>
<td style="border:0px solid white;"> <p style="line-height:1.8">
<b>Location</b>: Oeiras Site, Room 0B01, Bartolomeu Dias Wing,<br>(<img src="/facility/flowcytometry/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 4625)<br> 
<b>Software</b>: SpectroFlo, FlowJo, IDEAS<br>
</td>
</tr>
</table>
</html>

===== Workstation overview =====

The Moonwalk is a high-performance data analysis workstation dedicated to analysis of spectral flow cytometry data with **SpectroFlo**. 
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The workstation is also equipped with **FlowJo Software** with a shared license from the Flow Platform, and the **IDEAS software** to analyse your data from the [[imagestreamx|Amnis Image Stream]]. 
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It is available free of charge, but please remember to book your session in advance through the Agendo system.
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<html><a href="https://europe.agendoscience.com/calendar/?id=MTljYWxlbmRhcnM=" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=booking.png">  Moonwalk Booking</a></html>
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If you need to analyse your data in FlowJo in <html><span style="color:blueviolet;font-size:100%;">Lisbon site</span></html>, check the [[macflow|MacFlow workstation]].
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If you need to analyse your data in FlowJo and SpectroFlo **remotely**, check also the [[tango|Tango workstation]].

===== Useful Links =====  
  * <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.049_spetroflo_cytek_aurora_tutorial_users.pdf" target="_blank">SpectroFlo Tutorial</a></html>
  * <html><a href="https://www.flowjo.com/solutions/flowjo/downloads/tutorials" target="_blank"> FlowJo Tutorials</a></html>
  * <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=flowjo_basic_tutorial_2018.pdf" target="_blank"> FlowJo Basic Tutorial </a></html>
  * <html><a href="https://www.medizin.uni-tuebingen.de/files/download/8YWbqX1VelmV0EKRLxkr9apM/IDEAS-User-Manual_6.2.pdf" target="_blank">IDEAS User Manual</a></html>
  * <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.027_-_ideas_software_tutorial.pdf" target="_blank"> IDEAS Tutorial</a></html>

<html>
<img src="/facility/flowcytometry/lib/exe/fetch.php?media=tango_name_wiki.png">
</html>

===== Workstation overview =====

Tango is a **remote** high-performance data analysis workstation. 

If you have IGC credentials, you have access to **FlowJo Software** and also to **IDEAS software** and **Machine Learning** to analyse data from the [[imagestreamx|Amnis ImageStream]] 
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Please book on the Agendo calendar <html>
<a href="https://europe.agendoscience.com/calendar/?id=MThjYWxlbmRhcnM=" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=booking.png">   Tango Booking - Oeiras site Users</a>
</html>
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For users at the Lisbon site, access is limited to **IDEAS software** and **Machine Learning** to analyse data from the [[imagestreamx|Amnis Image Stream]] 
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Please book on the Agendo calendar <html>
<a href="https://europe.agendoscience.com/calendar/?id=NDIxY2FsZW5kYXJz" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=booking.png">   Tango Booking - Lisbon site Users</a>
</html>

The workstation is available for use free of charge, but please remember to book your session in advance in the Agendo system. 

===== How to access remotely? =====

Please follow the steps: <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.flow.999_-_tango_remote_workstation.pdf" target="_blank"> Tango Remote connection </a></html>.

===== Useful Links =====
  * <html><a href="https://www.flowjo.com/solutions/flowjo/downloads/tutorials" target="_blank"> FlowJo Tutorials</a></html>
  * <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=flowjo_basic_tutorial_2018.pdf" target="_blank"> FlowJo Basic Tutorial </a></html>
  * <html><a href="https://www.medizin.uni-tuebingen.de/files/download/8YWbqX1VelmV0EKRLxkr9apM/IDEAS-User-Manual_6.2.pdf" target="_blank">IDEAS Software User Manual</a></html>


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