✎ trainingselfsorting []

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To operate our equipments you must attend the 2,5 hour session on the Principles of Flow Cytometry. If you haven’t already, please [[TrainingPrinciplesFlow|book your Session]]. 

Training to self-operate the sorters requires attending a __second theoretical session on the Principles of Cell Sorting.__ 

This 2 hour session is open to anyone who wishes to understand how to sort, optimize their sorting experiments, or simply to improve their cell sorting skills. It covers the fundamental principles of FACS (Fluorescence Activated Cell Sorting), and all the concepts for better planning, executing, and analyzing the sorting experiments. 
Also, operating shared instruments requires discipline and rigorous training to assure the maintenance and well-functioning of our equipments. 


This theoretical session __is followed by a 2 hour session oh hands-on training__ with one of our staff member. You will learn how to setup the instrument for your own experiments, as well as troubleshooting problems when and if they occur.

After the training, you will be kept as an “in training” user on the Agendo booking system for the next 5 sessions, where UCF staff will supervise you during the sort.
Once you become an independent user on the Cell Sorting, you are free to book. Either way the core facility is ready to assist you in any setting up issue or troubleshooting while running the sorter.

====Be sure to read the following documents prior to your practical training:====

  * <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=SOP.UCF.003_-_BD_FACSDiva_Tutorial.pdf" target="_blank">DIVA Tutorial</a></html>
  * <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.035_-_sorting_procedures_bd_facsaria_iii_fusion_adapted_to_power_users.pdf" target="_blank">Sorting Procedures</a></html>

  * <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=sop.ucf.004_-_ucf_imm_user_guidelines.pdf" target="_blank">User Guidelines</a></html>
  * <html><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=prepare_samples" target="_blank">How to prepare samples for Flow Cytometry</a></html>

====Check our Cell Sorters for Power Users====

<html><td  style="border:0px solid white;">
<p style="text-align:left"><a href="https://imm.medicina.ulisboa.pt/facility/flowcytometry/doku.php?id=bd_facsaria_fusion" target="_blank"><img src="/facility/flowcytometry/lib/exe/fetch.php?media=fusion_wiki_name_2.png" width=150></a></p></td>
</html>

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